Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 327, P. 125325 - 125325
Published: Oct. 23, 2024
Language: Английский
Deleted Journal, Journal Year: 2024, Volume and Issue: 2(1), P. 10001 - 10001
Published: Jan. 1, 2024
Fibrosis is defined by scarring and tissue hardening caused excess deposition of extracellular matrix components, mainly collagens. A fibrotic response can occur in any the body final outcome an unbalanced reaction to inflammation wound healing induced a variety insults, including persistent infections, autoimmune reactions, allergic responses, chemical exposure, radiation, injury. The accumulation proteins replaces living disrupts architecture leading organ malfunction. remains major clinical therapeutic challenge has been estimated account for 45% deaths developed world. While advances regarding mechanistic knowledge on underlying cell biology alterations fibrosis have helped characterize main phases mediators involved, this not yielded significant progress treatment. Only recently, metabolic features associated begun emerge. This information, likely representing only tip iceberg, suggests that derangement key culprit pathophysiology fibrogenesis. Workshop Cellular Metabolic Bases Organ Fibrosis, International University Andalusia, Baeza, Spain, October 8–11, 2023 aimed discuss current novel perspectives mechanisms contributing development different organs tissues, with particular focus new methodological developments metabolomics strategies.
Language: Английский
Citations
0
AJP Gastrointestinal and Liver Physiology, Journal Year: 2024, Volume and Issue: 327(4), P. G608 - G622
Published: Aug. 13, 2024
Chemical inhibition of EEF1A1 decreases hepatic lipid droplet size and gene expression signatures associated with liver cell types that contribute to MASLD progression. Furthermore, changes in are primarily attributable hepatocytes cholangiocytes. This work highlights the therapeutic potential targeting setting MASLD, utility RNA-Seq deconvolution reveal valuable information about tissue type composition type-associated from bulk data.
Language: Английский
Citations
0
PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(9), P. e1012509 - e1012509
Published: Sept. 6, 2024
The replication organelle of hepatitis C virus (HCV), called membranous web, is derived from the endoplasmic reticulum (ER) and mainly comprises double membrane vesicles (DMVs) that concentrate viral complexes. It also tightly associates with lipid droplets (LDs), which are essential for virion morphogenesis. In particular acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), a rate-limiting enzyme in triglyceride synthesis, promotes early steps assembly. close proximity between ER membranes, DMVs LDs therefore permits efficient coordination HCV cycle. Here, we demonstrate exaggerated LD accumulation due to excessive expression DGAT1 isozyme, DGAT2, dramatically impairs formation web. This effect depended on enzymatic activity association whereas mere was not sufficient hamper RNA replication. Our lipidomics data indicate both infection DGAT2 overexpression induced biogenesis markedly increased phospholipids long chain polyunsaturated fatty acids, suggesting dual use these lipids their possible competition DMV biogenesis. On other hand, depleted specific phospholipids, particularly oleyl acyl chain-containing phosphatidylcholines, which, contrast, HCV-infected cells likely infection. conclusion, our results exchanges occurring during regulate composition intracellular membranes thereby affect organelle. potent antiviral observed system unveils flux may be relevant context steatohepatitis, hallmark infection, but physiological conditions, locally subdomains membrane. Thus, mediated by might participate spatial compartmentalization assembly factories within
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 12, 2024
Abstract Chromatin, with its complex spatial and temporal organization, plays a crucial role in regulating gene expression. Recent advancements super-resolution microscopy have revealed that nanoscale domains of heterochromatin (repressed segments) embedded within euchromatin (active background are fundamental units 3D chromatin organization. In tissue-resident cells, the size these varies microenvironment, particularly stiffness, organization is also influenced by pharmacological epigenetic drugs. However, mechanisms governing domain under various conditions their impact on expression remain unclear. To address this knowledge gap, we developed dynamic, next-generation sequencing informed copolymer model. Our model simulates spatiotemporal evolution chromatin, driven passive diffusion active reactions, which interconvert heterochromatin. By integrating chromatin-chromatin interaction energetics diffusion-reaction dynamics, predict formation heterochromatin-rich establish scaling relationship between modulation reaction rates. Additionally, our predicts compaction changes response to global rates occur predominantly at boundaries. We validated predictions via Hi-C contact map analysis imaging hyperacetylated melanoma cells. Subsequent RNA-seq suggested pivotal shifts influencing metastatic potential further mesoscale findings against rearrangement hMSCs, exhibit sensitivity microenvironmental stiffness. Finally, evaluated effects cycling silico, mimicking cellular transition different extracellular conditions, back again. This finding reveals cell-type invariant mechanism boundaries, whereby guides memory formation. show reorganization resulting from alterations drug exposure disease progression impacts both immediate responses long-term memory.
Language: Английский
Citations
0
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 327, P. 125325 - 125325
Published: Oct. 23, 2024
Language: Английский
Citations
0