Structure,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Highlights•A
software
suite
for
automated
analysis
of
lipid
membranes
in
electron
micrographs•Includes
segmentation,
shape
identification,
and
membrane
properties•Applied
to
datasets
with
different
lipids
protein-induced
changes•Features
an
intuitive
GUI
batch
micrograph
analysisSummaryImaging
structures
associated
protein
complexes
using
cryoelectron
microscopy
(cryo-EM)
is
a
common
visualization
structure
determination
technique.
The
quantitative
the
structures,
however,
not
routine
time
consuming
particular
when
large
amounts
data
are
involved.
Here,
we
introduce
image-processing
cryo-vesicle
image
analyzer
(CryoVIA)
that
parametrizes
from
cryo-EM
images.
This
toolkit
combines
identification
methods
automatically
perform
large-scale
local
global
properties
such
as
bilayer
thickness,
size,
curvature
including
classifications.
We
included
analyses
exemplary
compositions
changes
through
endosomal
sorting
required
transport
III
(ESCRT-III)
remodeling
protein.
opens
new
possibilities
systematically
study
structural
their
modifications
images.Graphical
abstract
Science,
Journal Year:
2024,
Volume and Issue:
385(6705), P. 168 - 174
Published: June 20, 2024
Intercellular
communication
in
the
nervous
system
occurs
through
release
of
neurotransmitters
into
synaptic
cleft
between
neurons.
In
presynaptic
neuron,
proton
pumping
vesicular-
or
vacuolar-type
ATPase
(V-ATPase)
powers
neurotransmitter
loading
vesicles
(SVs),
with
V
1
complex
dissociating
from
membrane
region
enzyme
before
exocytosis.
We
isolated
SVs
rat
brain
using
SidK,
a
V-ATPase–binding
bacterial
effector
protein.
Single-particle
electron
cryomicroscopy
allowed
high-resolution
structure
determination
V-ATPase
within
native
SV
membrane.
structure,
regularly
spaced
cholesterol
molecules
decorate
enzyme’s
rotor
and
abundant
protein
synaptophysin
binds
stoichiometrically.
ATP
hydrolysis
during
vesicle
results
loss
membrane,
suggesting
that
is
sufficient
to
induce
dissociation
enzyme.
Heliyon,
Journal Year:
2025,
Volume and Issue:
11(2), P. e41730 - e41730
Published: Jan. 1, 2025
Transient
Receptor
Potential
(TRP)
channels
are
a
family
of
ion
that
play
pivotal
roles
in
various
physiological
processes,
including
sensory
transduction,
temperature
regulation,
and
inflammation.
In
the
context
dentistry,
recent
research
has
highlighted
involvement
TRP
mediating
responses
inflammation
dental
tissues
temporo-mandibular
joint
(TMJ)
structure.
have
emerged
as
major
contributors
development
inflammatory
conditions
pain
affecting
oral
cavity
related
structures,
such
periodontitis,
erosion
cause
hypersensitivity,
pulpitis,
TMJ
disorders.
These
notably
contribute
to
health
challenges,
often
leading
sharp
pain,
dull
aches,
compromised
functionality.
Pharmacological
interventions
emerging
strategies
aimed
at
modulating
channel
activity
critically
evaluated.
The
therapeutic
potential
targeting
management
within
practice
is
focal
point
view
alleviate
conclusion,
this
comprehensive
review
provides
valuable
synthesis
current
knowledge
regarding
dentistry
underscoring
promising
targets
for
intervention,
then
paving
way
innovative
address
complexities
conditions.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 31, 2023
Membranes
are
molecular
interfaces
that
compartmentalize
cells
to
control
the
flow
of
nutrients
and
information.
These
functions
facilitated
by
diverse
collections
lipids,
nearly
all
which
distributed
asymmetrically
between
two
bilayer
leaflets.
Most
models
biomembrane
structure
function
often
include
implicit
assumption
these
leaflets
have
similar
abundances
phospholipids.
Here,
we
show
this
is
generally
invalid
investigate
consequences
lipid
abundance
imbalances
in
mammalian
plasma
membranes
(PM).
Using
quantitative
lipidomics,
discovered
cytoplasmic
human
erythrocyte
>50%
overabundance
phospholipids
compared
exoplasmic
This
imbalance
enabled
an
asymmetric
interleaflet
distribution
cholesterol,
regulates
cellular
cholesterol
homeostasis.
features
produce
unique
functional
characteristics,
including
low
PM
permeability
resting
tension
leaflet
protein
localization.
largely
overlooked
aspects
membrane
asymmetry
represent
evolution
classic
paradigms
physiology.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(29)
Published: July 10, 2024
In
this
study,
we
used
cryoelectron
microscopy
to
determine
the
structures
of
Flotillin
protein
complex,
part
Stomatin,
Prohibitin,
Flotillin,
and
HflK/C
(SPFH)
superfamily,
from
cell-derived
vesicles
without
detergents.
It
forms
a
right-handed
helical
barrel
consisting
22
pairs
Flotillin1
Flotillin2
subunits,
with
diameter
32
nm
at
its
wider
end
19
narrower
end.
Oligomerization
is
stabilized
by
C
terminus,
which
two
layers
linked
β-strand,
coiled-coil
domains
that
enable
strong
charge–charge
intersubunit
interactions.
interacts
membranes
both
ends;
through
SPFH1
wide
terminus
narrow
end,
facilitated
hydrophobic
interactions
lipidation.
The
inward
tilting
SPFH
domain,
likely
triggered
phosphorylation,
suggests
role
in
membrane
curvature
induction,
could
be
connected
proposed
clathrin-independent
endocytosis.
structure
shared
architecture
across
family
proteins
will
promote
further
research
into
Flotillin’s
roles
cell
biology.
Cancer Discovery,
Journal Year:
2024,
Volume and Issue:
14(4), P. 639 - 642
Published: April 4, 2024
Summary:
Cell
surface
proteins
represent
ideal
therapeutic
targets
because
of
their
accessibility
to
antibodies,
T
cell–directed
therapies,
and
radiotherapies,
but
there
are
only
25
therapeutically
relevant
cell
for
which
cancer
therapies
approved
in
the
United
States
or
European
Union.
This
commentary
calls
intensified
research
into
mapping
universe
–
surfaceome
order
accelerate
drug
development.
