Biomedicines,
Journal Year:
2024,
Volume and Issue:
13(1), P. 47 - 47
Published: Dec. 28, 2024
Gastrointestinal
tumors
present
a
significant
challenge
for
precision
medicine
due
to
their
complexity,
necessitating
the
development
of
more
specific
diagnostic
tools
and
therapeutic
agents.
Recent
advances
have
positioned
coding
non-coding
RNAs
as
emerging
biomarkers
these
malignancies,
detectable
by
liquid
biopsies,
innovative
Many
RNA-based
therapeutics,
such
small
interfering
RNA
(siRNA)
antisense
oligonucleotides
(ASO),
entered
clinical
trials
or
are
available
on
market.
This
review
provides
narrative
examination
potential
in
gastrointestinal
cancers,
with
an
emphasis
its
application
medicine.
discusses
current
challenges,
drug
resistance
tumor
metastasis,
highlights
how
molecules
can
be
leveraged
targeted
detection
treatment.
Additionally,
this
categorizes
targets
based
tissue
type,
offering
comprehensive
analysis
role
advancing
tumors.
Current Opinion in Chemical Biology,
Journal Year:
2024,
Volume and Issue:
81, P. 102506 - 102506
Published: Aug. 1, 2024
Despite
impressive
recent
establishment
of
therapeutic
nucleic
acids
as
drugs
and
vaccines,
their
broader
medical
use
is
impaired
by
modest
performance
in
intracellular
delivery.
Inefficient
endosomal
escape
presents
a
major
limitation
responsible
for
inadequate
cytosolic
cargo
release.
Depending
on
the
carrier,
this
barrier
can
strongly
limit
or
even
abolish
acid
Different
strategies
hypothesized
mechanisms
are
reviewed.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(11)
Published: March 4, 2024
Due
to
its
small
size
and
lifelong
optical
transparency,
the
fish
Danionella
cerebrum
is
an
emerging
model
organism
in
biomedical
research.
How
can
this
vertebrate
under
12
mm
length
produce
sounds
over
140
dB?
We
found
that
it
possesses
...Motion
basis
of
nearly
all
animal
behavior.
Evolution
has
led
some
extraordinary
specializations
propulsion
mechanisms
among
invertebrates,
including
mandibles
dracula
ant
claw
pistol
shrimp.
In
contrast,
...
Small,
Journal Year:
2024,
Volume and Issue:
20(42)
Published: June 25, 2024
Although
small-interfering
RNAs
(siRNAs)
are
specific
silencers
for
numerous
disease-related
genes,
their
clinical
applications
still
require
safe
and
effective
means
of
delivery
into
target
cells.
Highly
efficient
lipid
nanoparticles
(LNPs)
developed
siRNA
delivery,
showcasing
the
advantages
novel
pH-responsive
lipoamino
xenopeptide
(XP)
carriers.
These
sequence-defined
XPs
assembled
by
branched
lysine
linkages
between
cationizable
polar
succinoyl
tetraethylene
pentamine
(Stp)
units
apolar
fatty
acids
(LAFs)
at
various
ratios
bundle
or
U-shape
topologies.
Formulation
siRNA-LNPs
using
LAF
Biochemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 18, 2025
Efficient
delivery
of
siRNA-based
polyplexes
to
tumors
remains
a
major
challenge.
Nonspecific
interactions
in
the
bloodstream,
limited
circulation
time,
and
nontargeted
biodistribution
hamper
sufficient
tumor
accumulation.
To
address
these
challenges,
we
developed
an
ionic
hyaluronic
acid
(HA)
coating
shield
sequence-defined
oligoaminoamide-based
polyplexes.
This
should
positive
polyplex
surface
charge,
thus
reducing
nonspecific
enhancing
serum
stability.
Additionally,
modified
HA
with
cyclic
RGDfK
(cRGD)
peptide
specifically
target
endothelial
cells
(TECs).
Optionally,
polyethylene
glycol
(PEG)
spacer
was
also
introduced
improve
ligand
presentation
on
surface.
The
HA-coated
exhibited
favorable
physicochemical
properties,
including
negative
zeta
potential
effective
siRNA
retention
within
polyplex,
which
not
adversely
affected
by
PEG
or
cRGD
modification.
In
vitro
analyses
revealed
that
only
enhanced
cell
association
preserved
high
transfection
efficiency
plain
cationic
but
coating-dependent
cellular
internalization,
as
evidenced
competitive
inhibition
experiment.
Even
presence
serum,
encapsulated
effectively,
suitable
particle
sizes,
maintained
gene
silencing
efficiency.
vivo
studies
involving
intravenous
administration
into
Neuro2a
tumor-bearing
mice
showed
coating,
particularly
when
cRGD,
significantly
increased
accumulation
HA–PEG–cRGD-shielded
compared
Collectively,
our
results
indicate
superior
performance
terms
stability
uptake,
both
vivo.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 19, 2025
Abstract
Endothelial
cells
(EC)
comprise
the
pulmonary
vascular
bed
and
play
a
significant
role
in
health
disease.
Consequently,
EC
niche
represents
an
attractive
therapeutic
target
for
treating
wide
range
of
diseases.
We
have
identified
new
class
dicationic
Charge-Altering
Releasable
Transporters.
These
single-component
transporters
selectively
deliver
mRNA
to
lung
upon
intravenous
administration
without
use
targeting
ligand.
Significantly,
number
spatial
array
cationic
charges
within
repeating
units
CART
polymer
are
found
control
both
delivery
efficacy
tissue
tropism.
High-resolution
imaging
revealed
efficient
endothelial
arteries,
veins
capillaries.
The
selective
tropism
these
CARTs,
coupled
with
tunable
synthesis
this
family
amphiphiles,
enabling
platform
research
clinical
applications.
