Exploring the potential of seed inoculation with microbial consortia to mitigate drought stress in maize plants under greenhouse conditions

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Background and aims Plant-beneficial microbes may attenuate climate change-induced stresses on plants such as drought. We investigated the potential of beneficial microbial consortia (BMc) plant growth rhizosphere bacterial/archaeal community under Methods Seeds Zea mays B73 were inoculated with six plant-beneficial bacterial isolates either alone or combined in two three-member (BMc1, BMc2) before sowing loamy sandy substrates greenhouse. A known effective consortium (BMc3) was included positive control. Drought treatment established BMc treatments by omitting watering last five weeks period. The maize single determined. Colony-forming units (CFUs) inoculants evaluated selective plating, effects native assessed using 16S rRNA gene amplicon sequencing basal root tip grown loam. Results In both water conditions, CFUs inoculations higher at roots than tips. Under well-watered seed inoculation a isolate had no effect substrates. resulted shoot (but not root) compared to non-inoculated controls conditions zone most important driver for beta-diversity, followed while showed effect. Conclusion Our study suggests that has drought stress during growth.

Language: Английский

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Interplay of biotic and abiotic factors shapes tree seedling growth and root-associated microbial communities

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: March 22, 2024

Abstract Root-associated microbes can alleviate plant abiotic stresses, thus potentially supporting adaptation to a changing climate or novel environments during range expansion. While change is extending species fundamental niches northward, the distribution and colonization of mutualists (e.g., arbuscular mycorrhizal fungi) pathogens may constrain growth regeneration. Yet, degree which biotic factors impact performance associated microbial communities at edge their remains unclear. Here, we use root microscopy, coupled with amplicon sequencing, study bacterial, fungal, root-associated from sugar maple seedlings distributed across two temperate-to-boreal elevational gradients in southern Québec, Canada. Our findings demonstrate that soil pH, Ca, distance trees are key drivers communities, overshadowing influence elevation. Interestingly, changes fungal community composition mediate an indirect effect pH on seedling growth, pattern consistent both sites. Overall, our highlight complex role shaping tree-microbe interactions, turn correlated growth. These have important ramifications for tree expansion response shifting climatic niches.

Language: Английский

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Rhizosphere metabolomics reveals benzoxazinoid-mediated interspecific root interactions that promote N and Fe uptake in intercropped plants

Soil Biology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109792 - 109792

Published: March 1, 2025

Language: Английский

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Microbiome-mediated plant disease resistance: recent advances and future directions

Journal of General Plant Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Plant pathogens cause plant diseases that significantly decrease crop yields, posing a serious threat to global food security. While disease resistance has traditionally been understood as the trait determined by innate immune system and pathogen virulence system, recent research underscores pivotal role of microbiome in resistance. Plant-associated microbiomes confer protection against through direct inhibition, resource competition, activation responses. Agricultural practices such rotation, intercropping, disease-resistant breeding, biocontrol, organic farming modulate microbiomes, thereby influencing This review synthesizes latest advancements understanding intricate interactions among plants, pathogens, microbiomes. We emphasize need for in-depth mechanistic studies linking agricultural dynamics propose future directions leverage sustainable agriculture.

Language: Английский

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Nontarget impacts of neonicotinoids on nectar‐inhabiting microbes

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(3)

Published: March 1, 2024

Abstract Plant‐systemic neonicotinoid (NN) insecticides can exert non‐target impacts on organisms like beneficial insects and soil microbes. NNs affect plant microbiomes, but we know little about their effects microbial communities that mediate plant‐insect interactions, including nectar‐inhabiting microbes (NIMs). Here employed two approaches to assess the of NN exposure several NIM taxa. First, assayed in vitro six compounds growth using plate assays. Second, inoculated a standardised community into nectar NN‐treated canola ( Brassica napus ) assessed survival after 24 h. With few exceptions, tended decrease bacterial metrics. However, magnitude concentrations at which were observed varied substantially across bacteria. Yeasts showed no consistent response NNs. In nectar, saw treatment Rather, abundance diversity responded inherent qualities volume. conclusion, found evidence NIMs respond field‐relevant levels within h, our study suggests context, specifically assay methods, time traits, is important assaying communities.

Language: Английский

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Host genetic variation and specialized metabolites from wheat leaves enriches for phyllosphere Pseudomonas spp. with enriched antibiotic resistomes

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Antibiotic resistance in plant-associated microbiomes poses significant risks for agricultural ecosystems and human health. Although accumulating evidence suggests a role plant genotypes shaping their microbiome, almost nothing is known about how the changes of genetic information affect co-evolved microbiome carrying antibiotic genes (ARGs). Here, we selected 16 wheat cultivars experimentally explored impact host variation on phyllosphere ARGs, metabolites. Our results demonstrated that significantly influenced resistomes. Wheat exhibiting high ARGs were linked to elevated Pseudomonas populations, along with increased abundances aeruginosa biofilm formation genes. Further analysis 350 spp. genomes from diverse habitats at global scale revealed nearly all strains possess multiple virulence factor (VFGs), mobile elements (MGEs) genomes, albeit lower nucleotide diversity compared other species. These findings suggested proliferation contributed resistance. We further observed direct links between upregulated leaf metabolite DIMBOA-Glc, spp., enrichment which corroborated by microcosm experiments demonstrating DIMBOA-Glc enhanced relative abundance Overall, alterations metabolites resulting throughout evolution may drive development highly specialized microbial communities capable enriching ARGs. This study enhances our understanding plants actively shape clarifies

Language: Английский

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The lactonase BxdA mediates metabolic specialisation of maize root bacteria to benzoxazinoids

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 2, 2024

Abstract Root exudates contain specialised metabolites that shape the plant’s root microbiome. How host-specific microbes cope with these bioactive compounds, and how this ability affects microbiomes, remains largely unknown. We investigated maize bacteria metabolise benzoxazinoids, main of maize. Diverse abundant metabolised major compound in rhizosphere MBOA (6-methoxybenzoxazolin-2(3H)-one) formed AMPO (2-amino-7-methoxy-phenoxazin-3-one). forming were enriched benzoxazinoid-producing could use as carbon source. identified a gene cluster associated formation microbacteria. The first cluster, bxdA encodes lactonase converts to vitro. A deletion mutant homologous genes genus Sphingobium , did not form nor was it able BxdA different genera bacteria. Here we show plant-specialised select for metabolisation-competent represents benzoxazinoid metabolisation whose carriers successfully colonize thereby chemical environmental footprint.

Language: Английский

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Re-Envisioning the Plant Disease Triangle by Integration of Host Microbiota and a Pivot in Focus to Health Outcomes

Annual Review of Phytopathology, Journal Year: 2024, Volume and Issue: 62(1), P. 31 - 47

Published: April 29, 2024

The disease triangle is a structurally simple but conceptually rich model that used in plant pathology and other fields of study to explain infectious as an outcome the three-way relationship between host, pathogen, their environment. It also serves guide for finding solutions treat, predict, prevent such diseases. With omics-driven, evidence-based realization abundance activity pathogen are impacted by proximity interaction with diverse multitude microorganisms colonizing same evolved into tetrahedron shape, which features added fourth dimension representing host-associated microbiota. Another variant emerged from recently formulated pathobiome paradigm, deviates classical "one pathogen" etiology favor scenario represents conditional complex interactions among its microbiota (including microbes pathogenic potential), result version original where "pathogen" substituted "microbiota." Here, part careful concise review origin, history, usage triangle, I propose next step evolution, replace word "disease" center host-microbiota-environment triad "health." This highlights health desirable (rather than unwanted state) emergent property interactions. Applied discipline pathology, offers expanded range targets approaches diagnosis, prediction, restoration, maintenance outcomes. Its applications not restricted diseases only, underlying framework more inclusive all microbial contributions well-being, including those mycorrhizal fungi nitrogen-fixing bacteria, there never was proper place triangle. may have edge education communication tool convey stress importance healthy plants associated broader public stakeholdership.

Language: Английский

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An Isoflavone catabolism gene cluster underlying Interkingdom interactions in the soybean rhizosphere

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Plant roots secrete various metabolites, including plant specialized into the rhizosphere, and shape rhizosphere microbiome, which is crucial for health growth. Isoflavones are major metabolites found in legume plants, involved interactions with soil microorganisms as initiation signals rhizobial symbiosis modulators of root microbiota. However, it remains largely unknown molecular basis underlying isoflavone-mediated interkingdom rhizosphere. Here, we isolated Variovorax sp. strain V35, a member Comamonadaceae that harbors isoflavone-degrading activity, from soybean discovered gene cluster responsible isoflavone degradation named ifc. The characterization ifc mutants heterologously expressed Ifc enzymes revealed isoflavones undergo oxidative catabolism, different reductive metabolic pathways observed gut We further demonstrated genes frequently bacterial strains mutualistic rhizobia, contribute to detoxification antibacterial activity isoflavones. Taken together, our findings reveal an catabolism microbiota, providing insights legume–microbiota interactions.

Language: Английский

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Auxin-mediated regulation of susceptibility to toxic metabolites, c-di-GMP levels, and phage infection in the rhizobacterium Serratia plymuthica

mSystems, Journal Year: 2024, Volume and Issue: 9(7)

Published: June 5, 2024

ABSTRACT The communication between plants and their microbiota is highly dynamic involves a complex network of signal molecules. Among them, the auxin indole-3-acetic acid (IAA) critical phytohormone that not only regulates plant growth development, but emerging as an important inter- intra-kingdom modulates many bacterial processes are during interaction with hosts. However, corresponding signaling cascades remain largely unknown. Here, we advance our understanding unknown mechanisms by which IAA carries out its regulatory functions in plant-associated bacteria. We showed caused changes global transcriptome rhizobacterium Serratia plymuthica multidisciplinary approaches revealed sensing interferes mediated other pivotal plant-derived signals such amino acids 4-hydroxybenzoic acid. Exposure to large alterations transcript levels genes involved metabolism, resulting significant metabolic alterations. treatment also increased resistance toxic aromatic compounds through induction AaeXAB pump, confers IAA. Furthermore, promoted motility severely inhibited biofilm formation; phenotypes were associated decreased c-di-GMP capsule production. gene expression enhanced sensitivity capsule-dependent phage. Additionally, induced several antibiotic led susceptibility responses antibiotics different action. Collectively, study illustrates complexity IAA-mediated IMPORTANCE Signal plays role adaptation ecological niches This appears be particularly bacteria since they possess number transduction systems respond wide diversity chemical, physical, biological stimuli. key molecule variety processes. despite extensive knowledge plants, remains provide insight into primary secondary formation, motility, susceptibility, phage biocontrol rhizobacterium. work has implications for ecology environments biotechnological clinical applications IAA, well related

Language: Английский

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