Probiotics alleviate paraneoplastic thrombocythemia of ovarian cancer: A randomized placebo-controlled trial

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 119, P. 106316 - 106316

Published: June 25, 2024

Paraneoplastic thrombocytosis is common in ovarian cancer and often correlates with disease recurrence. This study aimed to assess the impact of probiotics on platelet counts patients. Enrolled patients paraneoplastic received oral Bifidobacterium longum JBLC-141 for level modulation. Over a 12-month period, were closely monitored recurrence rates. The revealed significant differences between probiotic placebo groups various parameters, including counts, coagulation functions, inflammatory factors, tumor markers, gut microbiota composition. However, no distinctions observed during postoperative stabilization. Oral effectively mitigated count surges improved functions thrombocytosis. Positive effects inflammation, marker levels, composition also noted. Further clinical validation needed confirm probiotics' potential extending stabilization period.

Language: Английский

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Trogocytosis in CAR immune cell therapy: a key mechanism of tumor immune escape

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 28, 2024

Immune cell therapy based on chimeric antigen receptor (CAR) technology platform has been greatly developed. The types of CAR immune have expanded from T cells to innate such as NK and macrophages, the diseases treated hematological malignancies non-tumor fields infectious autoimmune diseases. Among them, CAR-T CAR-NK observed examples rapid remission in approved clinical trials, but efficacy is unstable plagued by tumor resistance. Trogocytosis a special phenomenon intercellular molecular transfer that common system achieved recipient through acquisition internalization donor cell-derived molecules mediates effects. Recently, novel short-term drug resistance mechanism trogocytosis proposed, bidirectional exchange between triggered partially explains long-term relapse after treatment with cells. In this review, we summarize research progress immunotherapy, discuss influencing factors its direct indirect interference emphasize can further release potential therapy.

Language: Английский

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Real-time imaging reveals a role for macrophage protrusive motility in melanoma invasion

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 224(2)

Published: Nov. 21, 2024

Macrophages are primary cells of the innate immune system that mediate tumor progression. However, motile behavior macrophages and interactions with not well understood. Here, we exploit optical transparency larval zebrafish perform real-time imaging macrophage–melanoma interactions. We found highly in microenvironment. extend dynamic projections between precede invasive melanoma migration. Modulating macrophage motility a dominant inhibitory mutation Rac2 inhibits recruitment to impairs invasion. hyperactivating does affect but limits into mass reduces cell Taken together, these findings reveal role for Rac2-mediated protrusive

Language: Английский

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CSPG4-targeting CAR-macrophages inhibit melanoma growth

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

ABSTRACT Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of hematological malignancies but been clinically less effective in solid tumors. Engineering macrophages with CARs emerged as a promising approach to overcome some challenges faced by CAR-T cells due macrophage’s ability easily infiltrate tumors, phagocytose their targets, and reprogram immune response. We engineered CAR-macrophages (CAR-Ms) target chondroitin sulfate proteoglycan 4 (CSPG4), an expressed melanoma, several other CSPG4-targeting CAR-Ms exhibited specific phagocytosis CSPG4-expressing melanoma cells. Combining CD47 blocking antibodies synergistically enhanced CAR-M-mediated effectively inhibited spheroid growth 3D. Furthermore, tumor mouse models. These results suggest that CAR-M immunotherapy is for treating melanoma. STATEMENT OF SIGNIFICANCE alternative treatment. targeting CSPG4, phagocytosed vivo . Thus, may be strategy treat patients

Language: Английский

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Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

Abstract Macrophages often pervade solid tumors, but their nearest neighbor organization is understudied and potentially enables key functions such as phagocytosis. Here, we observe dynamic macrophage clusters in tumors under conditions that maximize cancer cell phagocytosis use reductionist approaches to uncover pathways cluster formation roles for tumor-intrusive pseudopodia, which term ‘intrudopodia’. Macrophage form over hours on low- adhesion substrates after M1 polarization with interferons, including T cell-derived cytokines, yet prove fluid timescales of minutes. Clusters also sort from M2 macrophages disperse the same substrates. upregulate specific cell-cell receptors suppress actomyosin contractility, while both contribute formation, decreased cortical tension was predicted unleash pseudopodia. neighbors tumor spheroids indeed extend intrudopodia between adjacent junctions – at least when are maximized, coordinated help detach individualize cells rapid engulfment. thereby provide a cooperative advantage overcome cohesion.

Language: Английский

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RAC1 serves as a prognostic factor and correlated with immune infiltration in liver hepatocellular carcinoma

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(9)

Published: Sept. 12, 2024

Language: Английский

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