Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

A hormone-to-neuropeptide pathway inhibits sexual receptivity in immature Drosophila females DOI Creative Commons
Jie Chen, Pei‐Wen Zhu,

Sihui Jin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(8)

Published: Feb. 21, 2025

Newborns, typically asexual, undergo a process of sexual transition to reach maturity, but the regulatory mechanism underlying this is not clear. Here, we studied how female behavior modulated during by hormones and neuromodulators in Drosophila . We found that neuropeptide Leucokinin (LK) inhibits receptivity specifically period immature females, younger or mature females. Moreover, steroid hormone ecdysone, which mainly synthesized ovary maturation, acts on LK neurons via ecdysone receptor suppress receptivity. further suppresses through its LKR central pC1 neurons, decision center for behavior. These findings reveal hormone-to-neuropeptide pathway maturation , shedding light coordinate development behaviors.

Language: Английский

Citations

0
Exploiting TRP channel diversity in insects: a pathway to next-generation pest management DOI
Amit Umesh Paschapur,

Marella Sai Manoj,

J. S. Pavan

et al.

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Language: Английский

Citations

0
A brief history of insect neuropeptide and peptide hormone research DOI Creative Commons
Dick R. Nässel

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing proctolin in 1975. The first 25 years, before Drosophila genome, were characterized by efforts to identify novel NPHs biochemical means, mapping their distribution neurons, neurosecretory cells, endocrine cells intestine. Functional studies predominantly dealing hormonal aspects peptides many employed ex vivo assays. With annotation more specifically receptors other insects, a new era followed. started matching NPH ligands orphan receptors, localize improved detection methods. Important advances made introduction rich repertoire innovative molecular genetic approaches interfere expression or function receptors. These methods enabled cell- circuit-specific interference signaling for assays determine roles behavior physiology, imaging neuronal activity, analysis connectivity peptidergic circuits. Recent have seen dramatic increase reports multiple functions development, physiology behavior. Importantly, we can now appreciate pleiotropic NPHs, as well functional “networks” where state dependent ensures behavioral plasticity systemic homeostasis.

Language: Английский

Citations

1
Male cuticular pheromones stimulate removal of the mating plug and promote re-mating through pC1 neurons inDrosophilafemales DOI Creative Commons
Minsik Yun, Do‐Hyoung Kim, Tal Soo Ha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 28, 2023

Abstract In birds and insects, the female uptakes sperm for a specific duration post-copulation known as ejaculate holding period (EHP) before expelling unused mating plug through ejection. this study, we found that Drosophila melanogaster females shortens EHP when incubated with males or mated shortly after first mating. This phenomenon, which termed m ale-induced E HP s hortening (MIES), requires Or47b+ olfactory ppk23+ gustatory neurons, activated by 2-methyltetracosane 7-tricosene, respectively. These odorants raise cAMP levels in pC1 responsible processing male courtship cues regulating receptivity. Elevated neurons reduce reinstate their responsiveness to cues, promoting re-mating faster study established MIES genetically tractable model of sexual plasticity conserved neural mechanism. Significance Statement Sexual plasticity, adaptation reproductive behavior social changes, was explored fruit fly, insect. Our findings revealed inseminated females, encountering another courting post-mating, shorten (EHP). Specific pathways phenomenon were identified, converging on brain-a circuit regulates activity. Odors associated shortening increased second messenger cAMP. The transient elevation heightened excitability facilitating prompt removal subsequent . behavioral provided framework understanding processes involved.

Language: Английский

Citations

2
Male cuticular pheromones stimulate removal of the mating plug and promote re-mating through pC1 neurons in Drosophila females DOI Creative Commons
Minsik Yun, Do‐Hyoung Kim, Tal Soo Ha

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: April 16, 2024

In birds and insects, the female uptakes sperm for a specific duration post-copulation known as ejaculate holding period (EHP) before expelling unused mating plug through ejection. this study, we found that Drosophila melanogaster females shortens EHP when incubated with males or mated shortly after first mating. This phenomenon, which termed m ale- i nduced E HP s hortening (MIES), requires Or47b+ olfactory ppk23+ gustatory neurons, activated by 2-methyltetracosane 7-tricosene, respectively. These odorants raise cAMP levels in pC1 responsible processing male courtship cues regulating receptivity. Elevated neurons reduce reinstate their responsiveness to cues, promoting re-mating faster study established MIES genetically tractable model of sexual plasticity conserved neural mechanism.

Language: Английский

Citations

0
Male cuticular pheromones stimulate removal of the mating plug and promote re-mating through pC1 neurons in Drosophila females DOI Creative Commons
Minsik Yun, Do‐Hyoung Kim, Tal Soo Ha

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Sept. 10, 2024

In birds and insects, the female uptakes sperm for a specific duration post-copulation known as ejaculate holding period (EHP) before expelling unused mating plug through ejection. this study, we found that Drosophila melanogaster females shortens EHP when incubated with males or mated shortly after first mating. This phenomenon, which termed m ale- i nduced E HP s hortening (MIES), requires Or47b+ olfactory ppk23+ gustatory neurons, activated by 2-methyltetracosane 7-tricosene, respectively. These odorants raise cAMP levels in pC1 responsible processing male courtship cues regulating receptivity. Elevated neurons reduce reinstate their responsiveness to cues, promoting re-mating faster study established MIES genetically tractable model of sexual plasticity conserved neural mechanism.

Language: Английский

Citations

0
Male cuticular pheromones stimulate removal of the mating plug and promote re-mating through pC1 neurons in Drosophila females DOI Open Access
Minsik Yun, Do‐Hyoung Kim, Tal Soo Ha

et al.

Published: Aug. 22, 2024

In birds and insects, the female uptakes sperm for a specific duration post-copulation known as ejaculate holding period (EHP) before expelling unused mating plug through ejection. this study, we found that Drosophila melanogaster females shortens EHP when incubated with males or mated shortly after first mating. This phenomenon, which termed m ale-induced E HP s hortening (MIES), requires Or47b+ olfactory ppk23+ gustatory neurons, activated by 2-methyltetracosane 7-tricosene, respectively. These odorants raise cAMP levels in pC1 responsible processing male courtship cues regulating receptivity. Elevated neurons reduce reinstate their responsiveness to cues, promoting re-mating faster study established MIES genetically tractable model of sexual plasticity conserved neural mechanism.

Language: Английский

Citations

0
Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

Citations

0