Metamorphosis of a unicellular green alga in the presence of acetate and a spatially structured three‐dimensional environment DOI Creative Commons
Trang Vuong, Prateek Shetty, Ece Kurtoglu

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Summary Photosynthetic protists, named microalgae, are key players in global primary production. The green microalga Chlamydomonas reinhardtii is a well‐studied model organism. In nature, it dwells acetate‐rich paddy rice soil, which not mimicked by standard liquid laboratory conditions. Here, we maintained the algae environment with spatially structured 3‐D components (S3‐D) and acetate recreating natural We perform transcriptome sequencing, immunoblotting, fluorescence electron microscopy, Raman microspectroscopy to characterize S3‐D vs homogeneous undergo metamorphosis‐like process when transitioned from aquatic lifestyle simulating soil. These conditions result reduced cell size cilia length, an enlarged eyespot many cells double‐layered walls. RNA‐Seq reveals alterations c . 2400 transcripts. Four photoreceptors including CRY‐DASH1 phototropin governing plastid metabolism along its altered their protein expression. Consequently, photosynthetic pigments, lipids starch levels vary as do distribution patterns. Fitness against antagonistic bacteria enhanced concurrently downregulation of involved Ca 2+ channel transcript. This study highlights profound impact initiating processes inaccessible under Thus, overexpression lines for certain naturally created.

Language: Английский

Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells DOI
Rodrigo E. Catalán, Alexandros A. Fragkopoulos, Antoine Girot

et al.

Nature Protocols, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Language: Английский

Citations

1
Nutrient stress triggers sugar-mediated carotenoid production in algal-bacterial interactions DOI
Abdalah Makaranga, Pannaga Pavan Jutur

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: March 1, 2025

Language: Английский

Citations

0
Mycetocola to the rescue DOI

Agustina Taglialegna

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(6), P. 324 - 324

Published: April 24, 2024

Language: Английский

Citations

0
A Necrotizing Toxin Promotes Pseudomonas syringae Infection Across Evolutionarily Divergent Plant Lineages DOI

Kristina Grenz,

Khong‐Sam Chia, Emma K Turley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

ABSTRACT The Pseudomonas syringae species complex harbors a diverse range of plant pathogenic bacteria. While much the current understanding P. is centered on interactions with flowering plants, less known about infection in evolutionarily divergent non-flowering lineages. Here, we took comparative evolutionary approach to understand how infects distantly related plants. We identify broad host isolates causing significant disease liverwort Marchantia polymorpha , fern Ceratopteris richardii and Nicotiana benthamiana which last shared common ancestor over 500 million years ago. demonstrate that phytotoxin enriched belonging phylogroup 2 clade are particularly virulent relying combination type-3 secreted effector proteins lipopeptide syringomycin. application purified syringomycin promotes necrosis tissues activates conserved genes associated redox regulation cell death. Toxin-deficient phylogroups normally unable infect thalli exhibit enhanced bacterial growth when supplemented exogenous syringomycin, further highlighting its role as host-range defining factor . Collectively our research reveals key for promoting colonization, works concert antagonize an exceptionally wide spectrum land

Language: Английский

Citations

0
Endophytic Microbes, Evolution and Diversification Of DOI
James F. White,

Mariusz Tadych,

Mónica S. Torres

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
Metamorphosis of a unicellular green alga in the presence of acetate and a spatially structured three‐dimensional environment DOI Creative Commons
Trang Vuong, Prateek Shetty, Ece Kurtoglu

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Summary Photosynthetic protists, named microalgae, are key players in global primary production. The green microalga Chlamydomonas reinhardtii is a well‐studied model organism. In nature, it dwells acetate‐rich paddy rice soil, which not mimicked by standard liquid laboratory conditions. Here, we maintained the algae environment with spatially structured 3‐D components (S3‐D) and acetate recreating natural We perform transcriptome sequencing, immunoblotting, fluorescence electron microscopy, Raman microspectroscopy to characterize S3‐D vs homogeneous undergo metamorphosis‐like process when transitioned from aquatic lifestyle simulating soil. These conditions result reduced cell size cilia length, an enlarged eyespot many cells double‐layered walls. RNA‐Seq reveals alterations c . 2400 transcripts. Four photoreceptors including CRY‐DASH1 phototropin governing plastid metabolism along its altered their protein expression. Consequently, photosynthetic pigments, lipids starch levels vary as do distribution patterns. Fitness against antagonistic bacteria enhanced concurrently downregulation of involved Ca 2+ channel transcript. This study highlights profound impact initiating processes inaccessible under Thus, overexpression lines for certain naturally created.

Language: Английский

Citations

0