Bioengineering, Journal Year: 2024, Volume and Issue: 11(10), P. 1041 - 1041
Published: Oct. 18, 2024
This study aimed to evaluate the osteogenic potential of mesenchymal stromal cell (MSC) spheroids combined with basic fibroblast growth factor (bFGF) in a mouse femur fracture model. To begin, MSC were generated, and expression key trophic factors (
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(28)
Published: July 2, 2024
Mesenchymal stem cells (MSCs) are essential in regenerative medicine. However, conventional expansion and harvesting methods often fail to maintain the extracellular matrix (ECM) components, which crucial for their functionality efficacy therapeutic applications. Here, we introduce a bone marrow-inspired macroporous hydrogel designed large-scale production of MSC–ECM spheroids. Through soft-templating approach leveraging liquid–liquid phase separation, engineer hydrogels with customizable features, including pore size, stiffness, bioactive ligand distribution, enzyme-responsive degradability. These tailored environments conducive optimal MSC proliferation ease harvesting. We find that soft enhance mechanotransduction MSCs, establishing standard hydrogel-based 3D cell culture. Within these hydrogels, MSCs exist as both cohesive spheroids, preserving innate vitality, migrating entities actively secrete functional ECM proteins. Additionally, also gentle, enzymatic method breaks down allowing secreted naturally form spheroids display heightened stemness differentiation capacity, mirroring benefits native milieu. Our research underscores significance sophisticated materials design nurturing distinct subpopulations, facilitating generation enhanced potential.
Language: Английский
Citations
12
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101531 - 101531
Published: Feb. 5, 2025
Three-dimensional (3D) printing technology has shown significant promise in the medical field, particularly orthopedics, prosthetics, tissue engineering, and pharmaceutical preparations. This review focuses on innovative application of 3D addressing challenges osteonecrosis femoral head (ONFH). Unlike traditional hip replacement surgery, which is often suboptimal for younger patients, offers precise localization necrotic areas ability to create personalized implants. By integrating advanced biomaterials, this a promising strategy approach early hip-preserving treatments. Additionally, 3D-printed bone engineering scaffolds can mimic natural environment, promoting regeneration vascularization. In future, potential extends combining with artificial intelligence optimizing treatment plans, developing materials enhanced bioactivity compatibility, translating these innovations from laboratory clinical practice. demonstrates how uniquely addresses critical ONFH treatment, including insufficient vascularization, poor mechanical stability, limited long-term success conventional therapies. introducing gradient porous scaffolds, bioactive material coatings, AI-assisted design, work outlines novel strategies improve interventions. These advancements not only enhance efficacy but also pave way findings into applications.
Language: Английский
Citations
2
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 16, 2025
Hydrogels are extensively utilized in stem cell-based tissue regeneration, providing a supportive environment that facilitates cell survival, differentiation, and integration with surrounding tissues. However, designing hydrogels for regenerating hard tissues like bone presents significant challenges. Here, we introduce macroporous spatiotemporally programmed mechanical properties cell-driven regeneration. Using liquid-liquid phase separation interfacial supramolecular self-assembly of protein fibres, the structure provide ample space to prevent contact inhibition during proliferation. The rigid fibre-coated pore shell provides sustained cues guiding osteodifferentiation protecting against loads. Temporally, hydrogel exhibits tunable degradation rates can synchronize new deposition some extent. By integrating localized heterogeneity, structures, surface chemistry, regenerative degradability, demonstrate efficacy these cell-encapsulated rabbit porcine models. This marks substantial advancement tailoring cell-assisted
Language: Английский
Citations
1
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(45)
Published: Sept. 10, 2024
Abstract Constructing hydrogels with spatially heterogeneous structures are crucial for unlocking novel applications. To this end, selectively removing a specific portion of by facile and intricate destructive strategies is worth exploring. Herein, “contact‐destructive” hydrogel actuator presented, composed dynamic network doped hydrophilic polyethylene glycol (PEG). The behavior the attributed to surface tension‐induced spreading effect enhanced water absorption due additive PEG. Parameters that act on these mechanisms used control destruction hydrogel. During process, exhibits locomotion routes predetermined graphic pattern aid 3D printing. Additionally, such self‐destructive can be terminated UV light irradiation when PEG replaced poly(ethylene glycol) diacrylate (PEGDA). Significantly, diverse applications including controllable collapse, self‐erasing, on‐demand cell release, realized These results demonstrate present has great values in soft robotics, anti‐counterfeiting, controlled drug delivery, other related fields.
Language: Английский
Citations
4
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 10, 2025
Bacterial infection, a complex wound microenvironment, and persistent inflammatory response in acute wounds can result delayed healing abnormal scar formation, thereby compromising the normal function aesthetic appearance of skin tissue. This issue represents one most challenging problems clinical practice. study aims to develop hydrogel dressing specifically designed for treatment wounds, providing immediate effective protection affected areas. innovation seeks offer novel advanced solution management wounds. In this study, composite scaffold was synthesized through reaction between oxidized glycyrrhizic acid carboxymethyl chitosan Schiff base. The material properties were systematically characterized, its biocompatibility antibacterial efficacy rigorously evaluated. A rat model established compare multiple groups, assessing impact on microenvironment repair. results demonstrated that OGA-CMCS exhibited excellent injectability, biocompatibility, properties. It capable enhancing which turn influenced polarization macrophages from M1 M2 phenotype, mitigating response, promoting angiogenesis granulation tissue regeneration, accelerating healing. successfully developed glycyrrhizin-based dressing, not only introduces innovative approaches emergency surface defects but also provides an experimental foundation. is anticipated contribute significantly addressing relevant challenges.
Language: Английский
Citations
0
Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107618 - 107618
Published: Jan. 1, 2025
Language: Английский
Citations
0
Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 630 - 646
Published: Feb. 16, 2025
Language: Английский
Citations
0
Nano Letters, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
In stem cell therapies, small extracellular vesicles (sEVs) are extremely limited in application due to their production. Here, we propose a new concept of "cellular self-stimulation" and develop cost-effective method for the preparation sEVs, which enables conversion cellular traction self-generated stimulation through piezoionic hydrogels enhances ability cells secrete sEVs by more than an order magnitude. The adherent leads deformation substrate, turn translates into millivolt-level electrical signal acting on itself, stimulating produce sEVs. These remain biologically intact have shown excellent efficacy vitro vivo assays, confirming superior therapeutic potential high concentrations This provides strong impetus development dissemination therapies.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: April 23, 2025
Abstract Basement membrane, the specialized extracellular matrix (ECM) that compartmentalizes endothelial and epithelial cells, is final frontier in bioengineering. The fibrillar collagens (Col I Col III) of interstitial are commonly used for tissue modeling, but networking scaffold basement membrane (human IV VI) remain elusive. Commercial hydrogels like Matrigel simulate ill-defined, dilute, variable contain murine proteins. Here, we investigated whether human mesenchymal stromal cells (MSCs) could be to produce 3D scaffolds amenable decellularization downstream applications Using a xeno-free, process, MSCs from placenta, umbilical cord, bone marrow adipose tissues were cultivated as adherent multilayers or free-floating spheroids. Matrix assembly was assessed daily by fluorescence imaging western blot, revealing de novo, systematic, development. Notably, these rich membrane-specific components VI. Interestingly, observed distinct combinations alpha chains other depending on their origin. Ongoing work aims combine this intrinsic patterning with strategic process parameters refine composition resemble target tissues, develop assays processes angiogenesis incorporate relevant biology readout.
Language: Английский
Citations
0
Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0