Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Mechanisms underlying functional axonal rewiring after adult mammalian central nervous system (CNS) injuries remain unclear partially due to limited models. Here we develop a mouse intracranial pre-olivary pretectal nucleus (OPN) optic tract injury model and demonstrate that Pten/Socs3 knockout CNTF expression in retinal ganglion cells (RGCs) promotes regeneration OPN reinnervation. Revealed by transmission electron microscopy, trans-synaptic labeling, electrophysiology, synapses are formed mainly intrinsically photosensitive RGCs, thereby restoring the pupillary light reflex (PLR). Moreover, combining with Lipin1 knockdown accelerates recovery achieves reconnection chronic injury. PLR can be further boosted increasing RGC photosensitivity melanopsin overexpression, it also enhanced treatment of voltage-gated calcium channel modulator augment presynaptic release. These findings highlight importance neuronal types activity for CNS injuries.

Language: Английский

Biological engineering approaches for modulating the pathological microenvironment and promoting axonal regeneration after spinal cord injury

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: May 12, 2025

Functional recovery following spinal cord injury (SCI) presents significant challenges and imposes a substantial burden on society. Current research primarily focuses minimizing damage promoting regeneration to enhance functional after SCI. Following SCI, secondary injuries such as mitochondrial dysfunction, vascular rupture, inflammatory responses, glial scarring occur in the lesion area, forming pathological microenvironment. These factors expand extent of damage, exacerbate severity, severely impede axonal Modulating microenvironment through various interventions may facilitate promote This article reviews influence advancements axon concerning response, scar formation Additionally, it integrates insights from bioengineering improve microenvironment, summarizing progress research. The review concludes with novel strategies for enhancing regeneration, offering fresh perspectives future investigations.

Language: Английский

Emerging therapeutic strategies for optic nerve regeneration

Trends in Pharmacological Sciences, Journal Year: 2024, Volume and Issue: 46(1), P. 45 - 61

Published: Dec. 17, 2024

Language: Английский