Serotonin signaling at cilia synapses
Current Opinion in Neurobiology,
Journal Year:
2025,
Volume and Issue:
92, P. 102994 - 102994
Published: March 12, 2025
Language: Английский
Synaptic vesicle-omics in mice captures signatures of aging and synucleinopathy
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: May 1, 2025
Neurotransmitter
release
occurs
through
exocytosis
of
synaptic
vesicles.
α-Synuclein's
function
and
dysfunction
in
Parkinson's
disease
other
synucleinopathies
is
thought
to
be
tightly
linked
vesicle
binding.
Age
the
biggest
risk
factor
for
synucleinopathy,
~15%
proteins
have
been
central
nervous
system
diseases.
Yet,
age-
disease-induced
changes
vesicles
remain
unexplored.
Via
systematic
analysis
at
ultrastructural,
protein,
lipid
levels,
we
reveal
specific
populations,
proteins,
lipids
over
age
wild-type
mice
α-synuclein
knockout
with
without
expression
human
α-synuclein.
Strikingly,
find
several
previously
undescribed
lacking
α-synuclein,
suggesting
that
loss
contributes
dysfunction.
These
findings
not
only
provide
insights
into
biology
mechanisms
but
also
serve
as
a
baseline
further
mechanistic
exploration
disease-related
alterations
Language: Английский
A correlative workflow streamlines synaptic imaging by cryo-electron tomography
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 3, 2025
Abstract
Despite
decades
of
intense
research,
the
molecular
organization
synapse
is
not
well
understood.
To
address
this
issue,
we
sought
to
develop
a
method
for
systematic
imaging
synapses
by
cryo-electron
tomography
(cryo-ET),
technology
capable
mapping
cellular
architecture
at
resolution.
Thinning
samples
cryo-focused
ion
beam
milling
prerequisite
high-quality
cryo-ET
imaging,
but
process
needs
be
guided
structures
interest.
allow
robust
synaptic
targeting,
established
correlative
cryo-light/electron
microscopy
approach
which
are
fluorescently
labeled
in
minimally
invasive
manner,
using
synthetic
binder
postsynaptic
scaffold
PSD-95
and
antibodies
against
presynaptic
protein
Synaptotagmin-1.
Cryo-ET
sites
colocalization
consistently
revealed
excitatory
synapses.
Our
allows
structural
studies
complexes
situ
,
facilitating
investigations
Language: Английский
The big chill: Growth of in situ structural biology with cryo-electron tomography
QRB Discovery,
Journal Year:
2024,
Volume and Issue:
5
Published: Jan. 1, 2024
Abstract
In
situ
structural
biology
with
cryo-electron
tomography
(cryo-ET)
and
subtomogram
averaging
(StA)
is
evolving
as
a
major
method
to
understand
the
structure,
function,
interactions
of
biological
molecules
in
cells
single
experiment.
Since
its
inception,
has
matured
some
stellar
highlights
further
opportunities
broaden
applications.
this
short
review,
I
want
provide
personal
perspective
on
developments
cryo-ET
have
seen
it
for
last
~20
years
outline
steps
that
led
success.
This
my
eyes
junior
researcher
view
present
past
hardware
software
cryo-ET.
Language: Английский