Diverse plant RNAs coat Arabidopsis leaves and are distinct from apoplastic RNAs DOI Creative Commons
Lucía Borniego, Meenu Singla-Rastogi, Patricia Baldrich

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(1)

Published: Jan. 3, 2025

Transgenic expression of a double-stranded RNA in plants can induce silencing homologous mRNAs fungal pathogens. Although such host-induced gene is well documented, the molecular mechanisms by which RNAs move from cytoplasm plant cells across plasma membrane both host cell and are poorly understood. Indirect evidence suggests that this transfer may occur at very early stage infection process, prior to breach wall, suggesting might be secreted onto leaf surfaces. To assess whether Arabidopsis possess mechanism for secreting surfaces, we developed protocol isolating surface separately intercellular (apoplastic) RNA. This yielded abundant displayed an banding pattern distinct apoplastic RNA, it directly rather than exuded through stomata or hydathodes. Notably, was not associated with either extracellular vesicles protein complexes; however, species longer 100 nucleotides could pelleted ultracentrifugation. Furthermore, pelleting inhibited divalent cation chelator EGTA, these form condensates on surface. These derived almost exclusively Arabidopsis, but come diverse genomic sources, including rRNA, tRNA, mRNA, intergenic microRNAs, small interfering RNAs, tRNAs especially enriched. We speculate endogenous plays important role assembly microbial communities

Language: Английский

Diverse plant RNAs coat Arabidopsis leaves and are distinct from apoplastic RNAs DOI Creative Commons
Lucía Borniego, Meenu Singla-Rastogi, Patricia Baldrich

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(1)

Published: Jan. 3, 2025

Transgenic expression of a double-stranded RNA in plants can induce silencing homologous mRNAs fungal pathogens. Although such host-induced gene is well documented, the molecular mechanisms by which RNAs move from cytoplasm plant cells across plasma membrane both host cell and are poorly understood. Indirect evidence suggests that this transfer may occur at very early stage infection process, prior to breach wall, suggesting might be secreted onto leaf surfaces. To assess whether Arabidopsis possess mechanism for secreting surfaces, we developed protocol isolating surface separately intercellular (apoplastic) RNA. This yielded abundant displayed an banding pattern distinct apoplastic RNA, it directly rather than exuded through stomata or hydathodes. Notably, was not associated with either extracellular vesicles protein complexes; however, species longer 100 nucleotides could pelleted ultracentrifugation. Furthermore, pelleting inhibited divalent cation chelator EGTA, these form condensates on surface. These derived almost exclusively Arabidopsis, but come diverse genomic sources, including rRNA, tRNA, mRNA, intergenic microRNAs, small interfering RNAs, tRNAs especially enriched. We speculate endogenous plays important role assembly microbial communities

Language: Английский

Citations

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