Comparative single-cell analysis of transcriptional bursting reveals the role of genome organization in de novo transcript origination DOI Creative Commons
UnJin Lee, Cong Li, Christopher B. Langer

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(18)

Published: April 30, 2025

Spermatogenesis is a key developmental process underlying the origination of newly evolved genes. However, rapid cell type–specific transcriptomic divergence Drosophila germline has posed significant technical barrier for comparative single-cell RNA-sequencing studies. By quantifying surprisingly strong correlation between species- and in three closely related species, we apply statistical procedure to identify core set 198 genes that are highly predictive type identity while remaining robust species-specific differences span over 25 30 My evolution. We then utilize classifications based on 198-gene show how transcriptional increases throughout spermatogenic time. After validating these cross-species using RNA fluorescence situ hybridization imaging, investigate influence genome organization molecular evolution spermatogenesis vis-a-vis bursting. first altering burst size contributes premeiotic transcription bursting frequency postmeiotic expression. report global autosomal vs. X chromosomal may arise stage preceding full testis organogenesis by showing evolutionarily conserved decreases X-linked kinetics all examined somatic types. Finally, provide evidence supporting cultivator model de novo gene demonstrating appearance testis-specific transcripts potentially provides short-range regulation neighboring genes’ properties during stages spermatogenesis.

Language: Английский

Comparative single-cell analysis of transcriptional bursting reveals the role of genome organization in de novo transcript origination DOI Creative Commons
UnJin Lee, Cong Li, Christopher B. Langer

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(18)

Published: April 30, 2025

Spermatogenesis is a key developmental process underlying the origination of newly evolved genes. However, rapid cell type–specific transcriptomic divergence Drosophila germline has posed significant technical barrier for comparative single-cell RNA-sequencing studies. By quantifying surprisingly strong correlation between species- and in three closely related species, we apply statistical procedure to identify core set 198 genes that are highly predictive type identity while remaining robust species-specific differences span over 25 30 My evolution. We then utilize classifications based on 198-gene show how transcriptional increases throughout spermatogenic time. After validating these cross-species using RNA fluorescence situ hybridization imaging, investigate influence genome organization molecular evolution spermatogenesis vis-a-vis bursting. first altering burst size contributes premeiotic transcription bursting frequency postmeiotic expression. report global autosomal vs. X chromosomal may arise stage preceding full testis organogenesis by showing evolutionarily conserved decreases X-linked kinetics all examined somatic types. Finally, provide evidence supporting cultivator model de novo gene demonstrating appearance testis-specific transcripts potentially provides short-range regulation neighboring genes’ properties during stages spermatogenesis.

Language: Английский

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