Rapid Construction of Liquid-like Surfaces via Single-Cycle Polymer Brush Grafting for Enhanced Antifouling in Microfluidic Systems DOI Creative Commons
Feng Wu, Jing Xu, Yuanyuan Liu

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(10), P. 1241 - 1241

Published: Oct. 9, 2024

Liquid-like surfaces have demonstrated immense potential in their ability to resist cell adhesion, a critical requirement for numerous applications across various domains. However, the conventional methodologies preparing liquid-like often entail complex multi-step polymer brush modification process, which is not only time-consuming but also presents significant challenges. In this work, we developed single-cycle strategy build by leveraging high-molecular-weight bis(3-aminopropyl)-terminated polydimethylsiloxane, significantly simplifies preparation process. The resultant surface endowed with exceptional slipperiness, effectively inhibiting bacterial colonization and diminishing adherence of platelets. Moreover, it offers promising implications reducing dependency on anticoagulants microfluidic systems constructed from PDMS, all while sustaining its antithrombotic attributes.

Language: Английский

Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions DOI Creative Commons

D. M. Li,

Kunjie Li, Jianquan Li

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(10)

Published: March 5, 2025

Enzymatic nucleic acid reaction is a fundamental tool in molecular biology. However, high-complexity enzymatic DNA reactions and assays are still challenging due to the difficulties integrating scaling up microscale units mixing tools. Here, we present scalable acoustofluidic platform featuring acoustic virtual stirrer (AVS) arrays, serving as stirrers increase efficiency of interfacial reactions. Analogous magnetic stirrers, AVS arrays perturb fluid through oscillating pressure nodes, controllable terms speeds amplitudes via modulation. By optimizing kinetics surface-tethered enzymes AVS, achieve 7.74% improvement stepwise yield synthesis. In addition, enhanced logic gate architecture can complete responses within 2 minutes, achieving average speed enhancement 8.58 times compared non-AVS configuration. With its tunability, ease integration, efficiency, this technology holds promises for applications biology chemistry.

Language: Английский

Citations

1
DNA-DISK: Automated end-to-end data storage via enzymatic single-nucleotide DNA synthesis and sequencing on digital microfluidics DOI Creative Commons
Kunjie Li, Xiaoyun Lu,

Jiaqi Liao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(34)

Published: Aug. 15, 2024

In the age of information explosion, exponential growth digital data far exceeds capacity current mainstream storage media. DNA is emerging as a promising alternative due to its higher density, longer retention time, and lower power consumption. To date, commercially mature synthesis sequencing technologies allow for writing reading on with customization convenience at research level. However, under disconnected nonspecialized mode, encounters practical challenges, including susceptibility errors, long latency, resource-intensive requirements, elevated security risks. Herein, we introduce platform named DNA-DISK that seamlessly streamlined synthesis, storage, microfluidics coupled tabletop device automated end-to-end storage. The single-nucleotide enzymatic biocapping strategy utilized, offering an ecofriendly cost-effective approach writing. A encapsulation using thermo-responsive agarose developed on-chip solidification, not only eliminating clutter but also preventing degradation. Pyrosequencing employed in situ accurate reading. As proof concept, successfully stored retrieved musical sheet file (228 bits) write-to-read latency (4.4 min per bit) well superior automation compared other platforms, demonstrating potential evolve into Hard Disk Drive future.

Language: Английский

Citations

5
Data Readout Techniques for DNA‐Based Information Storage DOI Open Access

B. Liu,

Fei Wang, Chunhai Fan

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

DNA is a natural chemical substrate that carries genetic information, which also serves as powerful toolkit for storing digital data. Compared to traditional storage media, molecules offer higher density, longer lifespan, and lower maintenance energy consumption. In process, data readout critical step bridges the gap between molecular/structures with stored information. With continued development of strategies in technology, techniques have evolved. However, there lack systematic introduction discussion on reported systems, especially correlation design system corresponding selection techniques. This review first introduces two main categories units (i.e., sequence structure) their sequencing nonsequencing methods), then reviewed representative examples notable advancements focusing unit design, technique selection. It emerging approaches assist techniques, such implementation microfluidic fluorescent probes. Finally, paper discusses limitations, challenges, potential approaches.

Language: Английский

Citations

0
Empowering DNA-Based Information Processing: Computation and Data Storage DOI
Kunjie Li, Heng Chen, Dayang Li

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Information processing is a critical topic in the digital age, as silicon-based circuits face unprecedented challenges such data explosion, immense energy consumption, and approaching physical limits. Deoxyribonucleic acid (DNA), naturally selected carrier for storing using genetic information, possesses unique advantages information processing, which has given rise to emerging fields of DNA computing storage. To meet growing practical demands, wide variety materials interfaces have been introduced into technologies, leading significant advancements. This review summarizes advances that facilitate computation We begin with brief overview fundamental functions principles Subsequently, we delve systems based on various interfaces, including microbeads, nanomaterials, nanostructures, hydrophilic–hydrophobic compartmentalization, hydrogels, metal–organic frameworks, microfluidics. also explore storage systems, encompassing encapsulation materials, microfluidics techniques, living cells. Finally, discuss current bottlenecks obstacles provide insights potential future developments.

Language: Английский

Citations

1
Rapid Construction of Liquid-like Surfaces via Single-Cycle Polymer Brush Grafting for Enhanced Antifouling in Microfluidic Systems DOI Creative Commons
Feng Wu, Jing Xu, Yuanyuan Liu

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(10), P. 1241 - 1241

Published: Oct. 9, 2024

Liquid-like surfaces have demonstrated immense potential in their ability to resist cell adhesion, a critical requirement for numerous applications across various domains. However, the conventional methodologies preparing liquid-like often entail complex multi-step polymer brush modification process, which is not only time-consuming but also presents significant challenges. In this work, we developed single-cycle strategy build by leveraging high-molecular-weight bis(3-aminopropyl)-terminated polydimethylsiloxane, significantly simplifies preparation process. The resultant surface endowed with exceptional slipperiness, effectively inhibiting bacterial colonization and diminishing adherence of platelets. Moreover, it offers promising implications reducing dependency on anticoagulants microfluidic systems constructed from PDMS, all while sustaining its antithrombotic attributes.

Language: Английский

Citations

0