An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish DOI Creative Commons
Leanne E. Iannucci, Varghese Thomas,

Micaela R. Murphy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

ABSTRACT Cell signaling regulates a wide range of biological processes including development, homeostasis, and disease. Accessible technologies to precisely manipulate have important applications in basic translational research. Here, we introduce an optogenetic toolkit comprised 1) zebrafish-optimized FGF activator, 2) single-transcript Nodal 3) previously established BMP activator. We thoroughly characterize this suite tools zebrafish embryos show that they provide tunable, light-dependent spatiotemporal control vivo . In response blue light (∼455 nm), receptor kinase domains fused light-dimerizing LOV enable robust activation with minimal ectopic activity the dark or at wavelengths over 495 nm. Optogenetic by each tool is pathway-specific results increased expression known target genes. Signaling activated rapid on/off kinetics, strength depends on irradiance. Finally, demonstrate spatially localized our optimized Together, establish as potent experimental platform rapidly, directly, adjustably activate FGF, BMP, embryos.

Language: Английский

In toto live imaging of Erk signaling dynamics in developing zebrafish hepatocytes DOI
Faraz Ahmed Butt, Alessandro De Simone, Stefano Di Talia

et al.

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish DOI Creative Commons
Leanne E. Iannucci, Varghese Thomas,

Micaela R. Murphy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

ABSTRACT Cell signaling regulates a wide range of biological processes including development, homeostasis, and disease. Accessible technologies to precisely manipulate have important applications in basic translational research. Here, we introduce an optogenetic toolkit comprised 1) zebrafish-optimized FGF activator, 2) single-transcript Nodal 3) previously established BMP activator. We thoroughly characterize this suite tools zebrafish embryos show that they provide tunable, light-dependent spatiotemporal control vivo . In response blue light (∼455 nm), receptor kinase domains fused light-dimerizing LOV enable robust activation with minimal ectopic activity the dark or at wavelengths over 495 nm. Optogenetic by each tool is pathway-specific results increased expression known target genes. Signaling activated rapid on/off kinetics, strength depends on irradiance. Finally, demonstrate spatially localized our optimized Together, establish as potent experimental platform rapidly, directly, adjustably activate FGF, BMP, embryos.

Language: Английский

Citations

0