MutLγ enforces meiotic crossovers in Arabidopsis thaliana DOI Creative Commons
Stéphanie Durand, Qichao Lian, Victor Solier

et al.

Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(5)

Published: Feb. 27, 2025

Abstract During meiosis, each chromosome pair experiences at least one crossover (CO), which directs their balanced segregation in addition to shuffling genetic information. COs tend be away from other, a phenomenon known as CO interference. The main biochemical pathway for formation, is conserved distant eukaryotes, involves the ZMM proteins together with MLH1–MLH3 complex (MutLγ). Here, we aim clarify role of MutLγ formation Arabidopsis thaliana. We show that AtMutLγ partially dispensable ZMM-dependent formation. HEI10 large foci—that mark sites wild-type—form normal level mlh1 and mlh3 mutants, but are inefficiently maturated into COs. Mutating MUS81 nuclease either or leads fragmentation, suppressed by further mutating zmm msh5. This suggests absence MutLγ, recombination intermediates produced ZMMs resolved MUS81, does not ensure Finally, interference marginally affected mlh1, compatible random sub-sampling normally patterned sites. conclude imposes designated exclusively COs, supporting view asymmetrically resolves double-Holliday junctions, yielding

Language: Английский

MutLγ enforces meiotic crossovers in Arabidopsis thaliana DOI Creative Commons
Stéphanie Durand, Qichao Lian, Victor Solier

et al.

Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(5)

Published: Feb. 27, 2025

Abstract During meiosis, each chromosome pair experiences at least one crossover (CO), which directs their balanced segregation in addition to shuffling genetic information. COs tend be away from other, a phenomenon known as CO interference. The main biochemical pathway for formation, is conserved distant eukaryotes, involves the ZMM proteins together with MLH1–MLH3 complex (MutLγ). Here, we aim clarify role of MutLγ formation Arabidopsis thaliana. We show that AtMutLγ partially dispensable ZMM-dependent formation. HEI10 large foci—that mark sites wild-type—form normal level mlh1 and mlh3 mutants, but are inefficiently maturated into COs. Mutating MUS81 nuclease either or leads fragmentation, suppressed by further mutating zmm msh5. This suggests absence MutLγ, recombination intermediates produced ZMMs resolved MUS81, does not ensure Finally, interference marginally affected mlh1, compatible random sub-sampling normally patterned sites. conclude imposes designated exclusively COs, supporting view asymmetrically resolves double-Holliday junctions, yielding

Language: Английский

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