Osteogenesis and Embryogenesis in Zebrafish Embryo Is Differentially Modulated by Solvents and Prednisolone DOI Creative Commons
Marta Carnovali, Giuseppe Banfi, Massimo Mariotti

et al.

Fishes, Journal Year: 2024, Volume and Issue: 9(12), P. 519 - 519

Published: Dec. 18, 2024

Several molecules and extracts are known to have bone-specific effects. For example, the long-term use of glucocorticoids like prednisolone causes several negative effects including a loss bone mass. Molecules usually dissolved in organic solvent which be toxic for zebrafish embryo certain concentrations. Nevertheless, solvents dimethyl sulfoxide (DMSO), ethanol methanol never been tested specific skeletal during development dose-dependency. Vitality assay, live fluorescence staining were used evaluate compared prednisolone. DMSO, perturb osteogenesis starting from 1%, 1.5% 3% respectively, concentrations vasculature, length survival rate appear unaffected. This effect may due high sensitivity process external chemical stimuli, especially trunk. On contrary, on appears more since it is found at very low concentrations, far any other developmental defects. The recommended concentration embryos assay was established 0.5% 2% ethanol. We recommend analyzing both head trunk mineralization assay.

Language: Английский

Letrozole induced a polycystic ovary syndrome model in zebrafish by interfering with the hypothalamic-pituitary-gonadal axis DOI

F. Zhang,

Chen Tang, Jingyi Wang

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 347, P. 123723 - 123723

Published: March 5, 2024

Language: Английский

Citations

3
Floral Fusion: Unravelling the Potent Blend of Ixora coccinea and Rhododendron arboreum for Health and Safety Benefits DOI Open Access

Pavithra Bharathy,

Punniyakoti T. Veeraveedu,

Arundhamizh N Shoban

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

and

Language: Английский

Citations

1
Reversal of neurodevelopmental impairment and cognitive enhancement by pharmacological intervention with the polyphenol polydatin in a Down syndrome model DOI
Marco Emili, Fiorenza Stagni, Carla Russo

et al.

Neuropharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 110170 - 110170

Published: Sept. 1, 2024

Language: Английский

Citations

0
Osteogenesis and Embryogenesis in Zebrafish Embryo Is Differentially Modulated by Solvents and Prednisolone DOI Creative Commons
Marta Carnovali, Giuseppe Banfi, Massimo Mariotti

et al.

Fishes, Journal Year: 2024, Volume and Issue: 9(12), P. 519 - 519

Published: Dec. 18, 2024

Several molecules and extracts are known to have bone-specific effects. For example, the long-term use of glucocorticoids like prednisolone causes several negative effects including a loss bone mass. Molecules usually dissolved in organic solvent which be toxic for zebrafish embryo certain concentrations. Nevertheless, solvents dimethyl sulfoxide (DMSO), ethanol methanol never been tested specific skeletal during development dose-dependency. Vitality assay, live fluorescence staining were used evaluate compared prednisolone. DMSO, perturb osteogenesis starting from 1%, 1.5% 3% respectively, concentrations vasculature, length survival rate appear unaffected. This effect may due high sensitivity process external chemical stimuli, especially trunk. On contrary, on appears more since it is found at very low concentrations, far any other developmental defects. The recommended concentration embryos assay was established 0.5% 2% ethanol. We recommend analyzing both head trunk mineralization assay.

Language: Английский

Citations

0