The first two chromosome-scale genome assemblies of American hazelnut enable comparative genomic analysis of the genusCorylus DOI Creative Commons
Scott Brainard, Dean Sanders, Tomáš Brůna

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 28, 2023

Summary The native, perennial shrub American hazelnut ( Corylus americana ) is cultivated in the Midwestern U.S. for its significant ecological benefits, as well high-value nut crop. Implementation of modern breeding methods and quantitative genetic analyses C. requires high-quality reference genomes, a resource that currently lacking. We therefore developed first chromosome-scale assemblies this species using accessions ‘Rush’ ‘Winkler’. Genomes were assembled HiFi PacBio reads Arima Hi-C data, Oxford Nanopore high-density map used to perform error correction. N50 scores are 31.9 Mb 35.3 Mb, with 90.2% 97.1% total genome into 11 pseudomolecules, ‘Winkler’, respectively. Gene prediction was performed custom RNAseq libraries protein homology data. has BUSCO score 99.0 assembly annotation, while ‘Winkler’ had corresponding 96.9 96.5, indicating assemblies. These two independent enable unbiased assessment structural variation within , patterns syntenic relationships across genus. Furthermore, we identified SNP marker sets from genotyping-by-sequencing data 1,343 americana, avellana x hybrids, order assess population structure natural populations. Finally, transcriptomes these assemblies, several other recently published utilized phylogenetic analysis sporophytic self-incompatibility (SSI) hazelnut, providing evidence unique molecular pathways governing .

Language: Английский

The first two chromosome-scale genome assemblies of American hazelnut enable comparative genomic analysis of the genusCorylus DOI Creative Commons
Scott Brainard, Dean Sanders, Tomáš Brůna

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 28, 2023

Summary The native, perennial shrub American hazelnut ( Corylus americana ) is cultivated in the Midwestern U.S. for its significant ecological benefits, as well high-value nut crop. Implementation of modern breeding methods and quantitative genetic analyses C. requires high-quality reference genomes, a resource that currently lacking. We therefore developed first chromosome-scale assemblies this species using accessions ‘Rush’ ‘Winkler’. Genomes were assembled HiFi PacBio reads Arima Hi-C data, Oxford Nanopore high-density map used to perform error correction. N50 scores are 31.9 Mb 35.3 Mb, with 90.2% 97.1% total genome into 11 pseudomolecules, ‘Winkler’, respectively. Gene prediction was performed custom RNAseq libraries protein homology data. has BUSCO score 99.0 assembly annotation, while ‘Winkler’ had corresponding 96.9 96.5, indicating assemblies. These two independent enable unbiased assessment structural variation within , patterns syntenic relationships across genus. Furthermore, we identified SNP marker sets from genotyping-by-sequencing data 1,343 americana, avellana x hybrids, order assess population structure natural populations. Finally, transcriptomes these assemblies, several other recently published utilized phylogenetic analysis sporophytic self-incompatibility (SSI) hazelnut, providing evidence unique molecular pathways governing .

Language: Английский

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