Venom component allergen IgE measurement in the diagnosis and management of insect sting allergy DOI Creative Commons
Simon Blank, Peter Korošec, Benjamin O. Slusarenko

et al.

The Journal of Allergy and Clinical Immunology In Practice, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Accurate identification of allergy-eliciting stinging insect(s) is essential to ensuring effective management Hymenoptera venom-allergic individuals with venom-specific immunotherapy. Diagnostic testing using whole-venom extracts skin tests and serologic-based analyses remains the first level discrimination for honeybee versus vespid venom sensitization in patients a positive clinical history. As second-level evaluation, serologic molecular allergens can further discriminate genuine (honeybee venom: Api m 1, 3, 4, 10 vs yellow jacket venom/Polistes dominula Ves v 1/Pol d 1 5/Pol 5) from interspecies cross-reactivity (hyaluronidases [Api 2, Pol 2] dipeptidyl peptidases IV 5, 3]). Clinical laboratories use number singleplex, oligoplex, multiplex immunoassays that employ both extracted (highlighted earlier) confirmation allergic sensitization. Established quantitative singleplex autoanalyzers have general governmental regulatory clearance worldwide patient maximally achievable analytical sensitivity (0.1 kUA/L) confirmed reproducibility (interassay coefficient variation <10%). Emerging oligoplex (fixed-panel) assays conserve on serum are more cost-effective, but they need some countries prone higher rates detecting asymptomatic Ultimately, patient's history, combined proof sensitization, final arbiter diagnosis allergy.

Language: Английский

Venom component allergen IgE measurement in the diagnosis and management of insect sting allergy DOI Creative Commons
Simon Blank, Peter Korošec, Benjamin O. Slusarenko

et al.

The Journal of Allergy and Clinical Immunology In Practice, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Accurate identification of allergy-eliciting stinging insect(s) is essential to ensuring effective management Hymenoptera venom-allergic individuals with venom-specific immunotherapy. Diagnostic testing using whole-venom extracts skin tests and serologic-based analyses remains the first level discrimination for honeybee versus vespid venom sensitization in patients a positive clinical history. As second-level evaluation, serologic molecular allergens can further discriminate genuine (honeybee venom: Api m 1, 3, 4, 10 vs yellow jacket venom/Polistes dominula Ves v 1/Pol d 1 5/Pol 5) from interspecies cross-reactivity (hyaluronidases [Api 2, Pol 2] dipeptidyl peptidases IV 5, 3]). Clinical laboratories use number singleplex, oligoplex, multiplex immunoassays that employ both extracted (highlighted earlier) confirmation allergic sensitization. Established quantitative singleplex autoanalyzers have general governmental regulatory clearance worldwide patient maximally achievable analytical sensitivity (0.1 kUA/L) confirmed reproducibility (interassay coefficient variation <10%). Emerging oligoplex (fixed-panel) assays conserve on serum are more cost-effective, but they need some countries prone higher rates detecting asymptomatic Ultimately, patient's history, combined proof sensitization, final arbiter diagnosis allergy.

Language: Английский

Citations

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