Natural resources, quantification, microbial bioconversion, and bioactivities of vitamin B12 for vegetarian diet

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 140849 - 140849

Published: Aug. 12, 2024

Language: Английский

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Unlocking the biotechnological potential of Decalepis arayalpathra: exploring synthetic seed production, metabolic profiling, genetic stability, and the impact of photosynthetic photon flux density on acclimatization

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 13, 2025

Decalepis arayapathra is an important medicinal plant known for several values, however, due to overharvesting, habitat destruction, and its limited geographical distribution, D. faces severe threats of extinction. A synthetic seed protocol was developed this plant, representing a novel approach in propagation conservation. Nodal segments (NS) were encapsulated sodium alginate (SA) matrix. 3% SA with 100 mM CaCl2 solutions best obtain ideal beads fine texture. Murashige Skoog (MS) medium consisting BA 5.0 µM + NAA 0.5 ADS 20.0 resulted maximum regrowth frequency 71.26% 3.13 shoots per bead shoot length 4.10 cm after six weeks culture. Rooting the microshoots better observed half- strength MS 2.5 NAA, resulting 3.1 roots microshoot root 3.0 four culture, followed by successful acclimatization. The study investigated effect photosynthetic photon flux density (PPFD) levels 50 300 PPFD on various physiological biochemical parameters during acclimatization vitro-derived plants. Results showed increase pigments, including chlorophyll carotenoids, as well enhanced net rate (PN) stomatal conductance (gs) prolonged acclimatization, higher being more effective. Antioxidant enzyme activities, SOD, CAT, APX, GR, increased over time, except which began decline 21 days under both light conditions. Stress markers such malondialdehyde (MDA) electrolyte leakage decreased indicating Genetic fidelity confirmed through clear monomorphic banding patterns obtained using RAPD ISSR markers. Quantification 2H4MB (2-hydroxy-4-methoxy benzaldehyde) synseed-derived HPLC revealed concentration 16.27 µg/ml. Metabolic profiling tuber GC-MS identified major minor metabolites. This offers breakthrough conservation technology, enabling sustainable while preserving genetic stability. It ensures consistent supply bioactive compound 2H4MB, promoting research commercial applications.

Language: Английский

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Function of Anthocyanin and Chlorophyll Metabolic Pathways in the Floral Sepals Color Formation in Different Hydrangea Cultivars

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 742 - 742

Published: Feb. 28, 2025

Hydrangea (Hydrangea macrophylla) is distinguished by having sepals instead of real petals, a trait that facilitates color diversity. Floral largely predetermined structural genes linked to anthocyanin production, but the genetic factors determining floral hue in this non-model plant remain unclear. Anthocyanin metabolites, transcriptome, and CIEL*a*b* system were employed elucidate biochemical molecular mechanisms formation three hydrangea cultivars: ‘DB’ (deep blue), ‘LB’ (light ‘GB’ (green blue). UPLC-MS/MS identified 47 with delphinidin, cyanidin, malvidin, petunidin, pelargonidin, peonidin being prominent. Delphinidins 90% primary component ‘DB’. The dataset identifies 51 31 DEGs associated anthocyanin, flavonoid, chlorophyll biosynthesis, CHS, CHI, F3H, F3′5′H, DFR, ANS, BZ1, 3AT displaying highest expression Notably, DFR (cluster-46471.3) exhibits high while down-regulated ‘GB’, correlating higher levels pigmentation. Comparative analyses vs. ‘DB’, revealed 460, 490, 444 differentially expressed TFs, respectively. WRKY, ERF, bHLH, NAC, AP2/ERF showed aligning key biosynthesis-related gene expression. findings reveal behind pigmentation variations lay groundwork for future breeding programs.

Language: Английский

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Light/dark cycles and iron supplementation to enhance the simultaneous production of polyhydroxyalkanoates, 5-aminolevulinic acid, coenzyme Q10, and pigments through photofermentation

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132513 - 132513

Published: April 1, 2025

This study aimed to obtain 5-aminolevulinic acid (5-ALA), Coenzyme Q10 (CoQ10), polyhydroxyalkanoates (PHA), carotenoids, and bacteriochlorophylls (Bchl) through the photofermentation of residual wine lees. Light/dark cycles iron supplementation were evaluated. Under 12-hour light/dark cycles, production 5-ALA, CoQ10, Bchl increased by 1.7, 2.8, 2.4 times, respectively, compared continuous illumination control, while PHA decreased from 511 445 mg/L. Combining with enhanced biomass rate. The CoQ10 content 4.9 times (reaching 8.8 mg/g-dw), carotenoids 3.7 (6.4 6.4 (17.9 mg/g-dw) control treatment, maintaining 5-ALA at 5.3 µmol/L 377 combination provides a triple benefit: value-added substances, rate, improved organic matter removal, making it an attractive option for winery effluent treatment valorization.

Language: Английский

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A new combination approach to extracellular production of 5-aminolevulinic acid for purification and application in alleviating cadmium-induced oxidative stress in maize

Protein Expression and Purification, Journal Year: 2025, Volume and Issue: unknown, P. 106736 - 106736

Published: May 1, 2025

Language: Английский

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Engineering Corynebacterium glutamicum for the Production of 5-Aminolevulinic Acid under Microaerobic Conditions Guided by a Genome-Scale Metabolic Network

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

5-Aminolevulinic acid (5-ALA) has been widely used in modern agriculture and therapy as a biostimulant, feed nutrient, photodynamic drug. Although metabolic engineering strategies have employed to increase the yield of 5-ALA Corynebacterium glutamicum, production under microaerobic conditions not studied. In this paper, we developed, for first time, overproducing-5-ALA glutamicum strains conditions, guided by genome-scale network model. The engineered strain C4 pathway synthesis was constructed based on model iCW773 different oxygen environmental conditions. fusion key enzymes SucCD HemA effectively opened substrate channel improved biosynthesis 5-ALA. Further selection synthetases alleviated inhibitory effect heme, which further titer Combinatorial optimization lpd, coaA, ppc genes enhance supply precursor succinyl-CoA. Finally, 3.8 g/L achieved 5-L bioreactor at 8% dissolved oxygen. This study provides reference or other high value-added chemicals with succinyl-CoA

Language: Английский

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Construction of Bacillus velezensis engineering strains for producing 5‐aminolevulinic acid to elicit plant resistance against abiotic stresses and tobacco bacterial wilt disease

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Abstract BACKGROUND Crops suffer from numerous biotic and abiotic stresses, leading to substantial economic losses globally. 5‐Aminolevulinic acid (ALA) has been shown stimulate plant resistance, thereby alleviating stress effects. RESULTS This study aimed develop stable multifaceted Bacillus velezensis strains with enhanced ALA production capabilities as potential biocontrol agents. To achieve this, the hemA gene sourced Bradyrhizobium japonicum was integrated into genomic DNA of by sequentially replacing existing genes bdh , pgsB sinI resulting in a recombinant strain, designated R9‐3, harboring three copies gene. R9‐3 achieved an titer approximately 22 mg/L. In effort further enhance accumulation, we disrupted downstream metabolic pathways deleting hemB, which is responsible for converting heme, sucCD participates diverting succinyl‐coenzyme A, key intermediate biosynthesis, away succinic production. Unexpectedly, these genetic modifications did not result additional increases proved efficacious mitigating detrimental effects salinity drought on tobacco plants. By stimulating activity vital antioxidative enzymes such catalase, peroxidase, superoxide dismutase, maintaining chlorophyll integrity, this strain reinforced plants' defense against oxidative under harsh environmental conditions. Moreover, resistance elicited effectively protected bacterial wilt disease. CONCLUSIONS These findings suggest that engineered significant be developed multi‐functional agent future agricultural applications, offering both protection. © 2025 Society Chemical Industry.

Language: Английский

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A novel frameshift mutation in ADCK1 identified in a case of chronic fatigue syndrome successfully treated with oral 5-ALA/SFC

Immunological Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 5

Published: Dec. 26, 2024

Chronic Fatigue Syndrome (CFS) is a complex disorder characterized by prolonged, unexplained fatigue and challenging diagnosis. We report the case of 35-year-old Japanese woman with CFS who had experienced chronic since age 11 years. Despite treatment modafinil, methylphenidate, levocarnitine, ubiquinone, symptoms persisted. Introduction oral 5-aminolevulinic acid sodium ferrous citrate (5-ALA/SFC) led to significant improvements in daily activities, mobility, psychosocial functioning. Genetic analysis revealed novel heterozygous frameshift deletion ADCK1 (p.Asn280fs), gene related mitochondrial function, which was confirmed using cDNA sequencing. deficiency has been associated developmental disabilities, dysfunction, increased reactive oxygen species levels, apoptosis Drosophila muscle cells. This supports hypothesis that mutations contribute dysfunction pathogenesis. The patient's clinical improvement 5-ALA/SFC ubiquinone suggests their potential for addressing dysfunction. Further functional familial analyses are required confirm role this mutation CFS. highlights importance considering CFS, therapeutic benefits ubiquinone.

Language: Английский

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