Enhanced Eicosapentaenoic Acid Production via Synthetic Biological Strategy in Nannochloropsis oceanica DOI Creative Commons

Congcong Miao,

Mingting Du, Hui Du

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(12), P. 570 - 570

Published: Dec. 19, 2024

The rational dietary ratio of docosahexaenoic acid (DHA) to eicosapentaenoic (EPA) can exert neurotrophic and cardiotrophic effects on the human body. marine microalga Nannochloropsis oceanica produces EPA yet no DHA, thus, it is considered an ideal EPA-only model pursue a DHA/EPA ratio. In this study, synthetic biological strategy was applied improve production in N. oceanica. Firstly, identify promoters terminators, fifteen genes from were isolated using transcriptomic approach. Compared α-tubulin, NO08G03500, NO03G03480 NO22G01450 exhibited 1.2~1.3-fold increases transcription levels. Secondly, EPA-synthesizing modules, putative desaturases (NoFADs) elongases (NoFAEs) overexpressed by NO08G03500 promoters/terminators wild type (WT), NoFAD1770 NoFAE0510 overexpression resulted 47.7% 40.6% yields, respectively. Thirdly, store triacylglycerol (TAG), NoDGAT2K promoter/terminator, along with NoFAD1770–NoFAE0510 stacking, forming transgenic line XS521. WT, TAG-EPA content increased 154.8% Finally, inhibit degradation, TAG lipase-encoding gene NoTGL1990 knocked out XS521, leading 49.2–65.3% increase content. Our work expands upon EPA-enhancing approaches through biology microalgae potentially crops.

Language: Английский

Machine learning-assisted synthetic biology of cyanobacteria and microalgae DOI

Weijia Jin,

Fangzhong Wang,

Lei Chen

et al.

Algal Research, Journal Year: 2025, Volume and Issue: unknown, P. 103911 - 103911

Published: Jan. 1, 2025

Language: Английский

Citations

0
Diurnal and Thermal Variations in an Oxidative Stress Biomarker of Pseudanabaena foetida: A Model for Environmental Adaptation in Aquatic Ecosystems DOI Creative Commons
Mizanur Rahman, Takashi Asaeda,

Kiyotaka Fukahori

et al.

Stresses, Journal Year: 2025, Volume and Issue: 5(1), P. 20 - 20

Published: March 3, 2025

Pseudanabaena foetida, a filamentous cyanobacterium, is highly sensitive to temperature and light intensity. This study explores its oxidative stress responses under diurnal intensities in variations, utilizing hydrogen peroxide (H2O2) normalized optical density (OD730) levels (H2O2/OD730) as biomarker. The adequate P. foetida cells were distributed three incubators for 22 days at 30 °C, 20 10 °C. Light intensity varied over the course of day, increasing from morning maximum noon, followed by gradual decrease until evening. Measurements H2O2, protein, chlorophyll (Chl a), catalase (CAT) activity carried out every hours, starting 6:00 ending 21:00. Protein concentration Chl increased between 12:00 15:00 temperature. CAT, H2O2/OD730 decreased afterwards each CAT was directly proportional foetida’s H2O2 or cell biomass (H2O2/OD730), which indicates defense mechanism. reduced (30 °C °C) significantly impactful on concentration, protein content. model, based biomass, provides valuable insights into different temperatures, with implications understanding climate change.

Language: Английский

Citations

0
Enhanced Eicosapentaenoic Acid Production via Synthetic Biological Strategy in Nannochloropsis oceanica DOI Creative Commons

Congcong Miao,

Mingting Du, Hui Du

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(12), P. 570 - 570

Published: Dec. 19, 2024

The rational dietary ratio of docosahexaenoic acid (DHA) to eicosapentaenoic (EPA) can exert neurotrophic and cardiotrophic effects on the human body. marine microalga Nannochloropsis oceanica produces EPA yet no DHA, thus, it is considered an ideal EPA-only model pursue a DHA/EPA ratio. In this study, synthetic biological strategy was applied improve production in N. oceanica. Firstly, identify promoters terminators, fifteen genes from were isolated using transcriptomic approach. Compared α-tubulin, NO08G03500, NO03G03480 NO22G01450 exhibited 1.2~1.3-fold increases transcription levels. Secondly, EPA-synthesizing modules, putative desaturases (NoFADs) elongases (NoFAEs) overexpressed by NO08G03500 promoters/terminators wild type (WT), NoFAD1770 NoFAE0510 overexpression resulted 47.7% 40.6% yields, respectively. Thirdly, store triacylglycerol (TAG), NoDGAT2K promoter/terminator, along with NoFAD1770–NoFAE0510 stacking, forming transgenic line XS521. WT, TAG-EPA content increased 154.8% Finally, inhibit degradation, TAG lipase-encoding gene NoTGL1990 knocked out XS521, leading 49.2–65.3% increase content. Our work expands upon EPA-enhancing approaches through biology microalgae potentially crops.

Language: Английский

Citations

0