RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum DOI Creative Commons
Sohaib Shahid, Muhammad Bilal Amir,

Tian‐Bo Ding

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 939 - 939

Published: Nov. 28, 2024

Neuropeptide CCHamide-1 (abbreviated as CCHa1) is a recently discovered peptide that present in many arthropods and the ligand of CCHa1R, member G protein-coupled receptors (GPCRs) superfamily, which plays regulatory role diverse physiological processes such feeding, circadian rhythm, insulin production, lipid metabolism, growth, reproduction. However, function this gene aphids still unknown. Here, we characterized determined potential CCHa1/CCHa1R signaling pea aphid, Acyrthosiphon pisum, notorious pest agriculture. The docking analysis revealed CCHa1 binds to its receptor CCHa1R through specific amino acid residues, are critical for maintaining structural functional integrity peptide–receptor complex. Quantitative real-time reverse transcription-PCR (qRT-PCR) expression levels transcripts different development stages tissues, indicating was high first nymphal instar compared upcoming instars adults, predominantly brain. transcript were significantly upregulated starved fed aphids. Moreover, RNAi knockdown by injection dsRNA-CCHa1 dsRNA-CCHa1R reduced corresponding target their food intake adult aphids, electrical penetration graph results. CCHa1/CCHa1R-silencing also reproduction, but not survival, A. pisum. Our data demonstrated play regulation feeding suggesting pathway relating nutritional status.

Language: Английский

Mutual coupling of neurons in the circadian master clock: what we can learn from fruit flies DOI Creative Commons
Charlotte Helfrich‐Förster, Nils Reinhard

Neurobiology of Sleep and Circadian Rhythms, Journal Year: 2025, Volume and Issue: 18, P. 100112 - 100112

Published: Jan. 18, 2025

Circadian master clocks in the brain consist of multiple neurons that are organized into populations with different morphology, physiology, and neuromessenger content presumably functions. In most animals, these distributed bilaterally, located close proximity to visual system, synchronized by eyes light-dark cycles environment. mammals cockroaches, each two consists a core region receives information from shell which output projections originate, whereas flies several other insects, lateral dorsal regions. all cases, morning evening clock seem exist, communication between them neurons, as well connection across hemispheres, is prerequisite for normal rhythmic function. Phenomena such rhythm splitting, internal desynchronization caused "decoupling" hemispheres or decoupling certain within one hemisphere. Since contain relatively few characterized at individual level, fly particularly suited study neurons. Here, we review organization bilateral brain, focus on synaptic paracrine connections comparison insects mammals.

Language: Английский

Citations

1
The Circadian Neuropeptide PDF has Sexually Dimorphic Effects on Activity Rhythms DOI Open Access
Aishwarya Ramakrishnan, Eva Scholz-Carlson, Evardra Valerie Bell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

The circadian system regulates the timing of multiple molecular, physiological, metabolic, and behavioral phenomena. In Drosophila as in other species, most research on how timekeeping brain controls outputs has been conducted males, or sex was not included a biological variable. main pacemaker neurons release neuropeptide Pigment Dispersing Factor (PDF), which functions key synchronizing factor network with complex effects clock neurons. Lack Pdf its receptor, PdfR, results flies displaying arrhythmicity activity-rest cycles under constant conditions. However, our show that female rhythms are less affected by mutations both PdfR. Crispr-Cas9 mutagenesis specifically ventral lateral (LNvs) also greater effect male rhythms. We tested influence M-cells over speeding up molecular leads to sexually dimorphic phenotypes, more pronounced rhythmic behavior. Our suggest is resilient manipulations PDF pathway distributed across neuron females.

Language: Английский

Citations

4
A Detailed Re-Examination of the Period Gene Rescue Experiments Shows That Four to Six Cryptochrome-Positive Posterior Dorsal Clock Neurons (DN1p) of Drosophila melanogaster Can Control Morning and Evening Activity DOI
Manabu Sekiguchi, Nils Reinhard,

Ayumi Fukuda

et al.

Journal of Biological Rhythms, Journal Year: 2024, Volume and Issue: 39(5), P. 463 - 483

Published: July 31, 2024

Animal circadian clocks play a crucial role in regulating behavioral adaptations to daily environmental changes. The fruit fly

Language: Английский

Citations

4
Adipocyte-Derived CCHamide-1, Eiger, Growth-Blocking Peptide 3, and Unpaired 2 Regulate Drosophila melanogaster Oogenesis DOI Creative Commons
Chad Simmons,

Isaiah Williams,

Tancia W. Bradshaw

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 513 - 513

Published: April 1, 2025

In addition to energy storage, adipose tissue communication other organs plays a key role in regulating organismal physiology. While the link between dysfunction and pathophysiology, including diabetes, chronic inflammation, infertility, is clear, molecular mechanisms that underlie these associations have not been fully described. We use Drosophila melanogaster as model better understand how communicates ovary. this study, we utilized D. melanogaster's robust genetic toolkit examine of five adipokines known control larval growth during development, CCHamide-1, CCHamide-2, eiger, Growth-blocking peptide 3, unpaired 2 oogenesis. show adult fat body expresses "larval" adipokines. Our data indicate ovarian germline stem cell maintenance does require However, adipocyte-derived influence early late survival well ovulation. Thus, work uncovers several mediate fat-to-ovary communication.

Language: Английский

Citations

0
Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations DOI Creative Commons
Sergio Hidalgo, Joanna C. Chiu

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 210(4), P. 585 - 599

Published: Aug. 16, 2023

Abstract Organisms adapt to unfavorable seasonal conditions survive. These adaptations rely on the correct interpretation of environmental cues such as photoperiod, and temperature. Genetic studies in several organisms, including genetic powerhouse Drosophila melanogaster , indicate that circadian clock components, period timeless are involved photoperiodic-dependent adaptations, but our understanding this process is far from complete. In particular, role temperature a key factor complement photoperiodic response not well understood. The development new sequencing technologies has proven extremely useful plastic changes other cellular components undergo different conditions, gene expression alternative splicing. This article discusses integration photoperiod for biology downstream molecular pathways regulation physiological occur with changing seasons. We focus discussion current involvement neuronal circuits these D. .

Language: Английский

Citations

5
Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

Citations

0
RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum DOI Creative Commons
Sohaib Shahid, Muhammad Bilal Amir,

Tian‐Bo Ding

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 939 - 939

Published: Nov. 28, 2024

Neuropeptide CCHamide-1 (abbreviated as CCHa1) is a recently discovered peptide that present in many arthropods and the ligand of CCHa1R, member G protein-coupled receptors (GPCRs) superfamily, which plays regulatory role diverse physiological processes such feeding, circadian rhythm, insulin production, lipid metabolism, growth, reproduction. However, function this gene aphids still unknown. Here, we characterized determined potential CCHa1/CCHa1R signaling pea aphid, Acyrthosiphon pisum, notorious pest agriculture. The docking analysis revealed CCHa1 binds to its receptor CCHa1R through specific amino acid residues, are critical for maintaining structural functional integrity peptide–receptor complex. Quantitative real-time reverse transcription-PCR (qRT-PCR) expression levels transcripts different development stages tissues, indicating was high first nymphal instar compared upcoming instars adults, predominantly brain. transcript were significantly upregulated starved fed aphids. Moreover, RNAi knockdown by injection dsRNA-CCHa1 dsRNA-CCHa1R reduced corresponding target their food intake adult aphids, electrical penetration graph results. CCHa1/CCHa1R-silencing also reproduction, but not survival, A. pisum. Our data demonstrated play regulation feeding suggesting pathway relating nutritional status.

Language: Английский

Citations

0