Identification and exploration of key genes associated with radioresistance in lung adenocarcinoma DOI Creative Commons
Ying Wang, Yangyang Shang,

Mingyu Hua

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 19, 2025

Radiation resistance in lung adenocarcinoma (LUAD) remains a primary obstacle limiting radiotherapy efficacy. However, the detailed factors and molecular mechanisms influencing LUAD radiosensitivity are not fully understood. Radioresistance-related genes (RRRGs) were screened by RNA sequencing bioinformatics analysis, prediction model for efficacy was developed via LASSO-Cox regression analysis. We specifically focused on Stanniocalcin 2 (STC2) due to its prognostic significance validated expression through immunohistochemical staining (IHC) pathological samples from patients. A STC2 knockdown (siSTC2) A549 cell line created, Western blotting, CCK8, colony formation assays performed investigate STC2's involvement radioresistance. An constructed using 6 RRRGs (FCGBP, SLCO4A1, ALDH3A1, STC2, TERT, CYP24A1). IHC analysis of 74 patients showed significantly higher non-responders (N-Res) versus responders (Res) (p < 0.05). Patients with elevated levels experienced shorter overall survival (OS). blotting revealed irradiated (IR) cells compared non-irradiated (N-IR) CCK8 results suggested that resulted significant reduction proliferation ability 0.05), confirmed decrease clonogenic siSTC2 controls plays role mediating radioresistance, high correlating poor prognosis. Therefore, represents promising therapeutic target overcoming Not applicable.

Language: Английский

Identification and exploration of key genes associated with radioresistance in lung adenocarcinoma DOI Creative Commons
Ying Wang, Yangyang Shang,

Mingyu Hua

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 19, 2025

Radiation resistance in lung adenocarcinoma (LUAD) remains a primary obstacle limiting radiotherapy efficacy. However, the detailed factors and molecular mechanisms influencing LUAD radiosensitivity are not fully understood. Radioresistance-related genes (RRRGs) were screened by RNA sequencing bioinformatics analysis, prediction model for efficacy was developed via LASSO-Cox regression analysis. We specifically focused on Stanniocalcin 2 (STC2) due to its prognostic significance validated expression through immunohistochemical staining (IHC) pathological samples from patients. A STC2 knockdown (siSTC2) A549 cell line created, Western blotting, CCK8, colony formation assays performed investigate STC2's involvement radioresistance. An constructed using 6 RRRGs (FCGBP, SLCO4A1, ALDH3A1, STC2, TERT, CYP24A1). IHC analysis of 74 patients showed significantly higher non-responders (N-Res) versus responders (Res) (p < 0.05). Patients with elevated levels experienced shorter overall survival (OS). blotting revealed irradiated (IR) cells compared non-irradiated (N-IR) CCK8 results suggested that resulted significant reduction proliferation ability 0.05), confirmed decrease clonogenic siSTC2 controls plays role mediating radioresistance, high correlating poor prognosis. Therefore, represents promising therapeutic target overcoming Not applicable.

Language: Английский

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