Whole‐Genome Sequencing and Functional Analysis of Klebsiella Strain mr‐1S Originating From Macrobrachium rosenbergii DOI Creative Commons
Xu Yang, Ying Zhao, Haihua Cheng

et al.

Aquaculture Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Klebsiella mr‐1S, a newly emerging pathogen that poses substantial threat to public health by inducing severe infectious diseases, has been detected in Macrobrachium rosenbergii for the first time our prior investigation. Given its potential environmental adaptation and genomic evolution, this study aims delve into these aspects better understand pathogenicity evolutionary trajectory. Methods: In study, isolates were subjected routine cultivation tryptic soya broth (TSB) medium. Subsequently, DNA of each isolate was individually extracted analyzed. Prior sequencing, integrity concentration samples meticulously evaluated ensure accuracy reliability subsequent sequencing process. Results: The sequence mr‐1S deciphered, revealing length 5,143,806 base pairs with GC content 54.97%. Remarkably, genome encompasses multitude putative mobile genetic elements (MGEs), including 43 islands (GIs) 2 prophages. These confer upon bacterium crucial adaptive attributes such as resistance, virulence, metabolic capabilities. Notably, identification prophage‐associated clusters originating from genus Pseudomonas suggests horizontal gene transfer (HGT) mechanism mediated phages within Pseudomonas, highlighting complex interactions between different bacterial species. Conclusion: addition, presence two genes encoding CRISPR‐Cas proteins indicates existence functional system bacterium. This finding implies during history, may have developed mechanisms evade host immune recognition, thereby facilitating efficient HGT enhancing adaptability survival capabilities diverse environments. Overall, provides novel insights plasticity , underscoring an aquatic ecosystems paving way future research on pathogenic control strategies.

Language: Английский

Whole‐Genome Sequencing and Functional Analysis of Klebsiella Strain mr‐1S Originating From Macrobrachium rosenbergii DOI Creative Commons
Xu Yang, Ying Zhao, Haihua Cheng

et al.

Aquaculture Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Klebsiella mr‐1S, a newly emerging pathogen that poses substantial threat to public health by inducing severe infectious diseases, has been detected in Macrobrachium rosenbergii for the first time our prior investigation. Given its potential environmental adaptation and genomic evolution, this study aims delve into these aspects better understand pathogenicity evolutionary trajectory. Methods: In study, isolates were subjected routine cultivation tryptic soya broth (TSB) medium. Subsequently, DNA of each isolate was individually extracted analyzed. Prior sequencing, integrity concentration samples meticulously evaluated ensure accuracy reliability subsequent sequencing process. Results: The sequence mr‐1S deciphered, revealing length 5,143,806 base pairs with GC content 54.97%. Remarkably, genome encompasses multitude putative mobile genetic elements (MGEs), including 43 islands (GIs) 2 prophages. These confer upon bacterium crucial adaptive attributes such as resistance, virulence, metabolic capabilities. Notably, identification prophage‐associated clusters originating from genus Pseudomonas suggests horizontal gene transfer (HGT) mechanism mediated phages within Pseudomonas, highlighting complex interactions between different bacterial species. Conclusion: addition, presence two genes encoding CRISPR‐Cas proteins indicates existence functional system bacterium. This finding implies during history, may have developed mechanisms evade host immune recognition, thereby facilitating efficient HGT enhancing adaptability survival capabilities diverse environments. Overall, provides novel insights plasticity , underscoring an aquatic ecosystems paving way future research on pathogenic control strategies.

Language: Английский

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