The alleviating effects and mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F combination on canine inflammatory bowel disease DOI Creative Commons
Rui Zhang, Wanjin Hu,

Saiwei Zhong

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: May 6, 2025

Canine inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation and dysbiosis. Conventional treatments often result in adverse effects contribute to antibiotic resistance, highlighting the need for safe, effective alternatives. Probiotics have gained attention their potential modulating gut microbiota immune responses. This study investigates therapeutic mechanisms of Enterococcus faecium Kimate-X Lactobacillus plantarum Kimate-F, individually combination, alleviating canine IBD. In vitro antibacterial anti-inflammatory activities were assessed using agar well diffusion assays LPS-induced RAW 264.7 macrophages, respectively. vivo efficacy was evaluated dextran sulfate sodium (DSS)-induced colitis models mice dogs. Metagenomic sequencing performed on fecal samples analyze composition functional pathways. Short-chain fatty acid (SCFA) levels quantified, key host signaling pathways examined. Kimate-F showed strong against Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica. significantly suppressed nitric oxide (NO) TNF-α production model. both mouse DSS-induced models, probiotic combination reduced weight loss, colonic damage, serum cytokines, while increasing IL-10 levels. analysis revealed enhanced microbial diversity, with enrichment Bifidobacterium species upregulation metabolic involved nutrient absorption regulation. The also modulated PPAR AMPK promoted SCFA feces. These findings suggest that E. L. act synergistically restore homeostasis, reduce inflammation, enhance immunity. Their ability modulate composition, signaling, output underscores as safe candidates managing

Language: Английский

The alleviating effects and mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F combination on canine inflammatory bowel disease DOI Creative Commons
Rui Zhang, Wanjin Hu,

Saiwei Zhong

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: May 6, 2025

Canine inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation and dysbiosis. Conventional treatments often result in adverse effects contribute to antibiotic resistance, highlighting the need for safe, effective alternatives. Probiotics have gained attention their potential modulating gut microbiota immune responses. This study investigates therapeutic mechanisms of Enterococcus faecium Kimate-X Lactobacillus plantarum Kimate-F, individually combination, alleviating canine IBD. In vitro antibacterial anti-inflammatory activities were assessed using agar well diffusion assays LPS-induced RAW 264.7 macrophages, respectively. vivo efficacy was evaluated dextran sulfate sodium (DSS)-induced colitis models mice dogs. Metagenomic sequencing performed on fecal samples analyze composition functional pathways. Short-chain fatty acid (SCFA) levels quantified, key host signaling pathways examined. Kimate-F showed strong against Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica. significantly suppressed nitric oxide (NO) TNF-α production model. both mouse DSS-induced models, probiotic combination reduced weight loss, colonic damage, serum cytokines, while increasing IL-10 levels. analysis revealed enhanced microbial diversity, with enrichment Bifidobacterium species upregulation metabolic involved nutrient absorption regulation. The also modulated PPAR AMPK promoted SCFA feces. These findings suggest that E. L. act synergistically restore homeostasis, reduce inflammation, enhance immunity. Their ability modulate composition, signaling, output underscores as safe candidates managing

Language: Английский

Citations

0