Mechanism of triptolide regulating proliferation and apoptosis of hepatoma cells by inhibiting JAK/STAT pathway

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Abstract This study was to analyze the effect of triptolide (TPL) on proliferation, apoptosis, and relationship between TPL Janus kinase/signal transducer activator transcription signaling pathway in hepatoma cells. HepG2 cell line selected as experimental object divided into control, low-dose TPL, medium-dose high-dose group. The control group did not receive any drug treatment, while low, medium, groups were treated with at concentrations 0.02, 0.05, 0.10 μM, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, flow cytometry, western blot used detach mechanism. proliferation inhibition rate each dose lower than that group, increased increase ( P < 0.05). apoptosis higher expression phosphorylated kinase 1 (p-JAK1) signal 3 (p-STAT3) protein cells p-JAK1 p-STAT3 decreased 10 ng/mL transforming growth factor-beta + reduced activity B-cell lymphoma/leukemia-2-associated X (Bax) cysteine aspartic acid protease (Caspase)-3/9 raised lymphoma/leukemia-2 (Bcl-2) With dose, Bax Caspase-3/9 increased, Bcl-2 As an anti-liver cancer agent, inhibits hepatocellular carcinoma promotes apoptosis. mechanism may involve inhibiting 1/signal activation apoptosis-related pathways.

Language: Английский

Sulforaphane from Brassica Oleracea Induces Apoptosis in Oral Squamous Carcinoma Cells via p53 Activation and Mitochondrial Membrane Potential Dysfunction

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 393 - 393

Published: March 11, 2025

Background/Objectives: Oral squamous cell carcinoma (OSCC) is a significant global health concern, necessitating the development of novel treatment strategies. The present study investigated in vitro anticancer activity sulforaphane (SFN), an isothiocyanate derived from Brassica oleracea, on OECM-1 human oral line. Methods: cells were cultured and exposed to range SFN concentrations. To assess viability determine half maximal inhibitory concentration (IC50) following 24 h treatment, MTT assay was performed. Apoptosis evaluated using AO/PI staining, TUNEL assay, Annexin V-FITC analysis, DNA fragmentation assay. Changes mitochondrial membrane potential analyzed JC-1 staining A Western blot performed expression levels apoptosis-associated proteins (Bax, Bcl2, caspase-3, caspase-9, PARP, Smad-4, p53, cytochrome c, GAPDH). Cell cycle analysis validate apoptotic findings. Results: IC50 5.7 µM. assays demonstrated effective induction apoptosis cells. dose-dependent upregulation Bax downregulation anti-apoptotic Bcl-2 Smad-4 after treatment. Conclusions: data obtained indicate that has induce by disrupting function modulating pathways. outcomes our research SFN’s as viable drug for OSCC.

Language: Английский

Phytomediated Chitosan‐Modified TiO₂ NPs for Enhanced Photocatalytic and Potential Application for Breast Cancer Therapy

Luminescence, Journal Year: 2025, Volume and Issue: 40(4)

Published: April 1, 2025

This work investigates the structural, photocatalytic, and biological properties of Juglans regia leaves extract mediated chitosan-modified TiO2 nanoparticles (NPs) NPs. The structural morphological analysis conducted using X-ray diffraction, scanning electron microscopy, transmission FTIR revealed that synthesizing NPs both chitosan results in lower particle size less agglomeration comparison to synthesized only extract. photocatalytic activity for degradation Rhodamine B phytomediated has a remarkable efficacy 95.07% when subjected UV-visible light. Further, have ability reduce E2 estrogen levels which inhibits growth breast cancer cells (MCF-7) was confirmed by yeast based XenoScreen XL YES assay, [3H] thymidine incorporation, apoptosis gene expression (Casp 3 Casp 9) analysis.

Language: Английский