Application of UHPLC-ESI-MS/MS-Based Metabonomic Techniques to Analyze the Cordyceps cicadae Metabolic Profile Changes to the CO(NH2)2 Response Mechanism in the Process of Ergosterol Synthesis DOI Creative Commons
Miaomiao Wang, Zhicai Zhang,

Fenghui Shi

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 90 - 90

Published: Feb. 11, 2025

Nitrogen source is a necessary nutrient factor in the process of mycelial growth and metabolite synthesis. To improve yield ergosterol, previous study, we used transcriptome technology to explore difference gene expression Cordyceps cicadae by CO(NH2)2 (urea) synthesizing ergosterol. In present was examine its effects on cell membrane permeability metabolic flow C. fermentation ergosterol Metabonomic results showed that supplementation caused significant changes five aspects: (1) notably increased biomass extracellular decreased intracellular concentration; (2) boosted level inositol 1,4-bisphosphate, which implied acceleration decomposition, weakening integrity, increase change regionalization; (3) changed fluxes speed, including increasing levels amino acids, vitamins, hormones, nucleotides, exhibited an elevated growth, promoting synthesis flavonoids, alkaloids, terpenes, facilitating decreasing degradation ergosterol; (4) anaphase, inhibition 6-phosphogluconate dehydrogenase α-ketoglutarate activities, NADPH, NADH, ATP synthesis, finally inhibited Collectively, metabonomics valuable strategy study regulatory medium composition incubation conditions cicadae.

Language: Английский

Application of UHPLC-ESI-MS/MS-Based Metabonomic Techniques to Analyze the Cordyceps cicadae Metabolic Profile Changes to the CO(NH2)2 Response Mechanism in the Process of Ergosterol Synthesis DOI Creative Commons
Miaomiao Wang, Zhicai Zhang,

Fenghui Shi

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 90 - 90

Published: Feb. 11, 2025

Nitrogen source is a necessary nutrient factor in the process of mycelial growth and metabolite synthesis. To improve yield ergosterol, previous study, we used transcriptome technology to explore difference gene expression Cordyceps cicadae by CO(NH2)2 (urea) synthesizing ergosterol. In present was examine its effects on cell membrane permeability metabolic flow C. fermentation ergosterol Metabonomic results showed that supplementation caused significant changes five aspects: (1) notably increased biomass extracellular decreased intracellular concentration; (2) boosted level inositol 1,4-bisphosphate, which implied acceleration decomposition, weakening integrity, increase change regionalization; (3) changed fluxes speed, including increasing levels amino acids, vitamins, hormones, nucleotides, exhibited an elevated growth, promoting synthesis flavonoids, alkaloids, terpenes, facilitating decreasing degradation ergosterol; (4) anaphase, inhibition 6-phosphogluconate dehydrogenase α-ketoglutarate activities, NADPH, NADH, ATP synthesis, finally inhibited Collectively, metabonomics valuable strategy study regulatory medium composition incubation conditions cicadae.

Language: Английский

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