A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure DOI Creative Commons
Muhammad K. Hakeem, Asma Al Menhali, Satheesh Elangovan

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Numerous physiological systems, such as the functioning of immune system, bone health, and regulation expression genes, depend critically on vitamin D. Considering significance D for it is critical to understand how metabolized factors that affect its levels. The objective this study was develop validate an LC-MS/MS method examine effects light exposure dietary consumption levels metabolites in a mouse model under consistent growth conditions throughout year. Serum hair samples from mice were analyzed various experimental using liquid chromatography-tandem mass spectrometry (LC-MS/MS). included D-deficient diet, D-standard changes ambient ranging complete darkness regular light-dark cycle. Mice fed standard diet exposed cycle exhibited significantly higher 25OHD3 both serum hair, indicating synergistic effect intake exposure. but kept continuous showed moderately elevated levels, demonstrating efficacy maintaining adequate despite absence light. Conversely, housed displayed below limit quantification, highlighting combined detrimental deficiency lack This provides valuable insights into complex interplay between intake, exposure, metabolism mice. Moreover, our results underscore potential implications human suggesting importance sunlight optimal Further research area has unveil additional influencing metabolism, offering strategies optimizing animal models subjects.

Language: Английский

A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure DOI Creative Commons
Muhammad K. Hakeem, Asma Al Menhali, Satheesh Elangovan

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Numerous physiological systems, such as the functioning of immune system, bone health, and regulation expression genes, depend critically on vitamin D. Considering significance D for it is critical to understand how metabolized factors that affect its levels. The objective this study was develop validate an LC-MS/MS method examine effects light exposure dietary consumption levels metabolites in a mouse model under consistent growth conditions throughout year. Serum hair samples from mice were analyzed various experimental using liquid chromatography-tandem mass spectrometry (LC-MS/MS). included D-deficient diet, D-standard changes ambient ranging complete darkness regular light-dark cycle. Mice fed standard diet exposed cycle exhibited significantly higher 25OHD3 both serum hair, indicating synergistic effect intake exposure. but kept continuous showed moderately elevated levels, demonstrating efficacy maintaining adequate despite absence light. Conversely, housed displayed below limit quantification, highlighting combined detrimental deficiency lack This provides valuable insights into complex interplay between intake, exposure, metabolism mice. Moreover, our results underscore potential implications human suggesting importance sunlight optimal Further research area has unveil additional influencing metabolism, offering strategies optimizing animal models subjects.

Language: Английский

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