Recent Progress in Physiological Significance and Biosynthesis of Lacto-N-triose II: Insights into a Crucial Biomolecule DOI

Cuie Guang,

Zhihui Du, Jiawei Meng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(36), P. 19539 - 19548

Published: Aug. 27, 2024

Lacto-

Language: Английский

High-Yield Synthesis of Lacto-N-Neotetraose from Glycerol and Glucose in Engineered Escherichia coli DOI
Yingxue Liao,

Caiwen Lao,

Jinyong Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(10), P. 5325 - 5338

Published: Jan. 26, 2024

Lacto-

Language: Английский

Citations

17
Engineering Escherichia coli for Highly Efficient Biosynthesis of Lacto-N-difucohexaose II through De Novo GDP-l-fucose Pathway DOI
Liang Wang, Yingying Zhu, Chunhua Zhao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(18), P. 10469 - 10476

Published: April 25, 2024

Lacto-N-difucohexaose II (LNDFH II) is a typical fucosylated human milk oligosaccharide and can be enzymatically produced from lacto-N-tetraose (LNT) by specific α1,3/4-fucosyltransferase Helicobacter pylori DMS 6709, referred to as FucT14. Previously, we constructed an engineered Escherichia coli BL21(DE3) with single plasmid for highly efficient biosynthesis of LNT. In this study, two additional plasmids harboring the de novo GDP-L-fucose pathway module FucT14, respectively, were further introduced construct strain successful LNDFH II. FucT14 was actively expressed, major product, lacto-N-fucopentaose (LNFP) V minor trace amount LNFP 3-fucosyllactose very products. Additional expression α1,3-fucosyltransferase FutM1 Bacteroidaceae bacterium gut metagenome could obviously enhance biosynthesis. After optimization induction conditions, maximum titer reached 3.011 g/L shake-flask cultivation. During fed-batch cultivation, efficiently highest 18.062 productivity yield 0.301 g/L·h.

Language: Английский

Citations

4
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design DOI
Yanfeng Liu, Guocheng Du, Jian Chen

et al.

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 93, P. 103315 - 103315

Published: May 9, 2025

Language: Английский

Citations

0
Structure, Function, Synthesis and Improved Strategies of Fucosylated Human Milk Oligosaccharides and Their Future Perspectives: A Review DOI

Ya-Ya Yang,

Shunli Jing,

Le Zhang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106584 - 106584

Published: April 1, 2025

Language: Английский

Citations

0
Iterative multimodule reprogramming and protein engineering for efficient lactodifucotetraose bioproduction DOI
Mengli Li, Tao Zhang, Chenchen Li

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105364 - 105364

Published: Oct. 1, 2024

Language: Английский

Citations

1
Recent Progress in Physiological Significance and Biosynthesis of Lacto-N-triose II: Insights into a Crucial Biomolecule DOI

Cuie Guang,

Zhihui Du, Jiawei Meng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(36), P. 19539 - 19548

Published: Aug. 27, 2024

Lacto-

Language: Английский

Citations

0