Transcriptomic Analysis across Crayfish (Cherax quadricarinatus) Claw Regeneration Reveals Potential Stem Cell Sources for Cultivated Crustacean Meat

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8623 - 8623

Published: Aug. 7, 2024

In the face of rising global demand and unsustainable production methods, cultivated crustacean meat (CCM) is proposed as an alternative means to produce delicious lobster, shrimp, crab products. Cultivated requires starting stem cells that may vary in terms potency propensity proliferate or differentiate into myogenic (muscle-related) tissues. Recognizing regenerating limbs are a non-lethal source tissue harbor relevant cells, we selected those crayfish Cherax quadricarinatus our model. To investigate cell activity, conducted RNA-Seq analysis across six stages claw regeneration (four pre-molt two post-molt stages), along with histology real-time quantitative PCR (qPCR). Our results showed while genes related energy production, muscle hypertrophy, exoskeletal cuticle synthesis dominated stages, growth factor receptors (FGFR, EGFR, TGFR, BMPR) proliferation (Cyclins, CDKs, Wnts, C-Myc, Klf4, Sox2, PCNA, p53) were upregulated before molt. Pre-molt upregulation several occurred peaks; Stages 2 4. We therefore propose limb tissues, particularly larger Stage 4, present prolific for CCM development.

Language: Английский

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Challenges in cellular agriculture: lessons from Pacific white shrimp, Litopenaeus vannamei

In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Language: Английский

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Effect of blocking transforming growth factor-β/Activin-Myostatin signaling on the expression of ecdysteroid metabolism and responsive genes in the crustacean molting gland (Y-organ)

General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: 362, P. 114675 - 114675

Published: Feb. 1, 2025

Language: Английский

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Advancing marine invertebrate cell line research: four key knowledge gaps

In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

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Artificial intelligence and machine learning applications for cultured meat

Frontiers in Artificial Intelligence, Journal Year: 2024, Volume and Issue: 7

Published: Sept. 24, 2024

Cultured meat has the potential to provide a complementary industry with reduced environmental, ethical, and health impacts. However, major technological challenges remain which require time-and resource-intensive research development efforts. Machine learning accelerate cultured technology by streamlining experiments, predicting optimal results, reducing experimentation time resources. use of machine in is its infancy. This review covers work available date on explores future possibilities. We address four areas development: establishing cell lines, culture media design, microscopy image analysis, bioprocessing food processing optimization. In addition, we have included survey datasets relevant CM research. aims foundation necessary for both scientists identify opportunities at intersection between learning.

Language: Английский

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Transcriptomic Analysis across Crayfish (<i>Cherax quadricarinatus</i>) Claw Regeneration Reveals Potential Stem Cell Sources for Cultivated Crustacean Meat

Published: July 3, 2024

In the face of rising global demand and unsustainable production methods, cultivated crustacean meat (CCM) is proposed as an alternative means to produce delicious lobster, shrimp crab products. Cultivated requires starting stem cells that may vary in terms potency, propensity proliferate or differentiate into myogenic (muscle-related) tissues. We suggest regenerating limbs harbor a range suitable for present humane tissue source because they can be accessed non-lethally. To investigate cell activity this model, we have conducted RNA-Seq analysis across six stages claw regeneration crayfish Cherax quadricarinatus (four pre-molt two post-molt stages), along with histology real time quantitative PCR (qPCR). Differential expression revealed patterns significantly up- down-regulated genes 14 functional categories. Relative select target (including endocrine, myogenic, cycle pluripotency factors) highlighted growth peaks during limited after molt. Histology qPCR results support overall finding limb more activity, likely making them prolific CCM development.

Language: Английский

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An overview of recent progress in the molecular mechanisms and key biological macromolecules involved in limb regeneration of decapods

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 292, P. 139354 - 139354

Published: Dec. 30, 2024

Language: Английский

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Editorial: Novel trends in cultured meat research

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: July 16, 2024

Language: Английский

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