Cytokine, Journal Year: 2025, Volume and Issue: 192, P. 156969 - 156969
Published: May 28, 2025
Language: Английский
Molecular and Cellular Probes, Journal Year: 2025, Volume and Issue: 79, P. 102007 - 102007
Published: Jan. 6, 2025
Language: Английский
Citations
2
Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(3)
Published: March 1, 2025
ABSTRACT Rheumatoid arthritis (RA) is a prevalent and debilitating autoimmune disease. Numerous studies have demonstrated promising results regarding the use of probiotics as therapeutic approach to alleviate RA symptoms. This study isolated monocytes from PBMCs patients healthy donors. These were then differentiated into macrophages divided five groups: untreated, LPS‐treated, L. delbrueckii (Del)‐treated, rhamnosus (Ram)‐treated, mixed treatment group. Three macrophage subpopulations—M0, M1, M2—were identified in all groups, with variations observed population percentages each subpopulation expression levels CD14, CD80, HLA‐DR. Flow cytometry indicated that, compared untreated LPS‐treated probiotic bacteria (Del, Ram, Mix) stimulated polarization toward M2 phenotype while suppressing percentage M1 population. Additionally, Pathogen‐Associated Molecular Pattern (PAMP) phagocytosis‐inducing receptor, was significantly reduced probiotic‐treated groups. Probiotic also profoundly influenced antigen presentation by ligand for CD28 co‐stimulatory marker on T cells, HLA‐DR, which presents antigens cell receptors Th4 cells. Interestingly, quantitative real‐time PCR that increased IL‐10 TGF‐β, both anti‐inflammatory cytokines, decreasing inflammatory including IL‐12, IL‐1β, TNF‐α, controls patients. It seems these may regulatory effect macrophages, affecting their polarization, patterns, phagocytosis, cytokine secretion profiles. suggests prophylactic effects RA.
Language: Английский
Citations
1
Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown
Published: April 2, 2025
Spinal cord injury (SCI) is a serious neurological disease with an extremely high disability rate. Most patients show loss of motor and sensory functions below the level injury. Current treatment protocols are based on early surgical decompression pharmacotherapy. However, efficacy these interventions suboptimal. Due to its complex pathophysiological mechanisms difficulty central nervous system (CNS) regeneration, exploring effective therapeutic remains daunting. Flavonoids secondary metabolites unique plants that have attracted attention in recent years for their potential now commonly used inflammation, tumors, other diseases. For SCI, related studies still exploring; some compounds, such as quercetin, fisetin, hesperetin, shown good anti-inflammatory anti-apoptotic properties, which help restore function injured spinal cord. flavonoids exhibit certain disadvantages, including poor solubility, low bioavailability, inability achieve long-term controlled release. Some proposed drug delivery strategies-including nanoparticles, hydrogels, collagen scaffolds-to enhance efficacy. In this paper, we summarize strategies SCI by searching relevant literature propose future research directions provide new ideas multimodal SCI.
Language: Английский
Citations
1
Brain Research Bulletin, Journal Year: 2025, Volume and Issue: unknown, P. 111272 - 111272
Published: March 1, 2025
Ferroptosis and inflammation are central to the pathophysiology of hyperhomocysteinemia (HHcy)-associated neurological disorders. Tectorigenin, a natural flavonoid aglycone extracted from numerous plants, possesses antioxidant, anti-inflammatory, neuroprotective properties. This study aimed investigate whether tectorigenin mitigates elevated homocysteine (Hcy)-induced toxicity in BV-2 microglial cells, focusing on its effects ferroptosis. Cell viability, lactate dehydrogenase (LDH) release, proliferation assays were employed evaluate cell injury. Inflammatory cytokines levels determined by ELISA. markers, including reactive oxygen species (ROS), lipid ROS, malondialdehyde (MDA), 4-hydroxy-nonenal (4-HNE), mitochondrial membrane potential (MMP), ATP, Fe2+ content, antioxidant enzymes (superoxide dismutase [SOD] catalase [CAT]) activities evaluated. mRNA protein expressions analyzed qRT-PCR western blotting, respectively. Our findings revealed that pretreatment significantly alleviated Hcy-induced injury inflammatory response cells. Furthermore, reduced peroxidation, enhanced capacity, ferroptotic death Hcy-treated Importantly, ferroptosis inhibitor Fer-1 also inflammation. Mechanistically, activated SIRT1/SLC7A11 pathway, silencing SIRT1 reversed protective effects. Collectively, these results indicate attenuates inhibiting through activation pathway.
Language: Английский
Citations
0
Toxicology Mechanisms and Methods, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 40
Published: May 5, 2025
Micro- and nanoplastics (MNPs), pervasive environmental pollutants, contaminate water, soil, air, the food chain ultimately accumulate in living organisms. Macrophages are main immune cells that gather around MNPs engulf them through process of phagocytosis. This internalization triggers M1 polarization secretion inflammatory cytokines, including IL-1, IL-18, IL-12, TNF-α, IFN-γ. Furthermore, damage mitochondria lysosomes, causing overactivation iNOS excessive production ROS. results cellular stress induce apoptosis, necroptosis, and, some cases, metosis macrophages. The also increases expression receptors, involving CD36, SR-A, LOX-1, macrophage receptor with a collagenous structure (MARCO) while decreasing ABCA-1 ABCG-1. adipose tissue macrophages trigger proinflammatory cytokine secretion, adipogenesis, lipid accumulation, insulin resistance, cytokines adipocytes. Various factors influence rate MNP by macrophages, size, charge, concentration, which affect passive diffusion. Receptor-mediated phagocytosis occurs directly via receptors like T-cell immunoglobulin mucin domain containing 4 (TIM-4) MARCO. attachment biomolecules, proteins, antibodies, opsonins, or microbes to (forming corona structures) promotes indirect receptor-mediated endocytosis, as possess TLRs FcγRIII. cause gut dysbiosis, risk factor for microenvironment polarization. Here, we review mechanisms consequences exposure, is linked autoimmunity, inflammation, cardiometabolic syndrome manifestations, atherosclerosis obesity, highlighting immunotoxicity MNPs.
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 19, 2025
The leaves of Shiliangcha (an alternative tea, Chimonanthus salicifolius), a perennial bush cultivated in Lishui, Zhejiang, have been used for thousands years by the She ethnic group as an herb and tea. salicifolius flavonoids (CsFE) exhibited remarkable antiaging properties. Therefore, we established d-galactose (d_Gal)-induced aging mouse model to investigate effect CsFE on central nervous system (CNS) hosts. Supplementation with effectively alleviated symptoms mice, including weight loss, declining learning memory capacity, blood-brain barrier (BBB) integrity, release pro-inflammatory cytokines, oxidative stress, neuroinflammation, microglia activation. Additionally, cognitive deficits interfering synaptic plasticity-associated protein levels, altering neuronal excitability, affecting intracellular neurotransmitters glutamate (Glu) γ-aminobutyric acid (GABA) release. Furthermore, supplementation modulated gut microbiota composition enriching probiotics Akkermansia, Muribaculaceae, Lactobacillus, Lachnospiraceae, promoting production short-chain fatty acids (SCFAs). this study suggested that has potential resist brain through intervention microbiota-gut-brain axis (GBA), which provides theoretical basis development natural drugs dietary supplements antiaging.
Language: Английский
Citations
0
Cytokine, Journal Year: 2025, Volume and Issue: 192, P. 156969 - 156969
Published: May 28, 2025
Language: Английский
Citations
0