NDM-5-carried outer membrane vesicles impair the efficacy of antibiotics against bacterial infections DOI Creative Commons
Lin Li, Yanfang Zhang,

Lihui Weng

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

ABSTRACT The intensifying use of antimicrobials in the rapidly growing livestock industry has heightened concerns over proliferation antibiotic resistance, particularly among Enterobacteriaceae producing β-lactamase. Elucidating role β-lactamase could unlock novel strategies to combat drug-resistant and poultry farming. Outer membrane vesicles (OMVs) produced by gram-negative bacteria have ability encapsulate transport components derived from their parental bacteria. This raises intriguing possibility that OMVs harbor drug-resistance enzymes, thereby conferring protection susceptible against antibiotics. Here, we successfully extracted New Delhi metallo-β-lactamase-5 (NDM-5)-expressing Escherichia coli confirmed these indeed carry NDM-5 protein. Furthermore, bacterial assays showed cause sensitive treated with meropenem restore growth activity, degradation was verified using high-performance liquid chromatography. Lastly, survival rate intervention group significantly lower than drug-treated a Galleria mellonella larvae infection model, validating protective effect on increasing tolerance meropenem. These findings illustrate can serve as vehicles for resistance-related factors, promoting

Language: Английский

NDM-5-carried outer membrane vesicles impair the efficacy of antibiotics against bacterial infections DOI Creative Commons
Lin Li, Yanfang Zhang,

Lihui Weng

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

ABSTRACT The intensifying use of antimicrobials in the rapidly growing livestock industry has heightened concerns over proliferation antibiotic resistance, particularly among Enterobacteriaceae producing β-lactamase. Elucidating role β-lactamase could unlock novel strategies to combat drug-resistant and poultry farming. Outer membrane vesicles (OMVs) produced by gram-negative bacteria have ability encapsulate transport components derived from their parental bacteria. This raises intriguing possibility that OMVs harbor drug-resistance enzymes, thereby conferring protection susceptible against antibiotics. Here, we successfully extracted New Delhi metallo-β-lactamase-5 (NDM-5)-expressing Escherichia coli confirmed these indeed carry NDM-5 protein. Furthermore, bacterial assays showed cause sensitive treated with meropenem restore growth activity, degradation was verified using high-performance liquid chromatography. Lastly, survival rate intervention group significantly lower than drug-treated a Galleria mellonella larvae infection model, validating protective effect on increasing tolerance meropenem. These findings illustrate can serve as vehicles for resistance-related factors, promoting

Language: Английский

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