Identification of novel antisense long non-coding RNA APMAP-AS that modulates porcine adipogenic differentiation and inflammatory responses DOI Creative Commons

Lin-zhen ZHANG,

Li HE,

Ning Wang

et al.

Journal of Integrative Agriculture, Journal Year: 2022, Volume and Issue: 22(8), P. 2483 - 2499

Published: Nov. 16, 2022

Long non-coding RNAs (lncRNAs) are emerging as powerful regulators of adipocyte differentiation, fat metabolism and gene expression. However, the functional roles mechanisms lncRNAs in these processes remain unclear. Here, we identified a novel antisense transcript, named APMAP-AS, transcribed from membrane-associated protein (APMAP) pig genome. APMAP-AS APMAP were highly expressed retroperitoneal adipose obese pigs, compared with that control pigs. Using bone mesenchymal stem cells adipogenic differentiation model, found positively regulated differentiation. had potential to form an RNA-RNA duplex APMAP, increased stability mRNA. Additionally, promoted lipid inhibited expression inflammatory factors. These findings natural transcript for regulatory associated synthesis might further our understanding driving adaptive tissue remodeling preserving metabolic health.

Language: Английский

Non-Coding RNAs and Adipogenesis DOI Open Access
Wenxiu Ru, Sihuan Zhang, Jianyong Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 9978 - 9978

Published: June 10, 2023

Adipogenesis is regarded as an intricate network in which multiple transcription factors and signal pathways are involved. Recently, big efforts have focused on understanding the epigenetic mechanisms their involvement regulation of adipocyte development. Multiple studies investigating regulatory role non-coding RNAs (ncRNAs) adipogenesis been reported so far, especially lncRNA, miRNA, circRNA. They regulate gene expression at levels through interactions with proteins, DNA, RNA. Exploring mechanism developments field RNA may provide a new insight to identify therapeutic targets for obesity related diseases. Therefore, this article outlines process adipogenesis, discusses updated roles ncRNAs development adipocytes.

Language: Английский

Citations

12
A new phytopharmaceutical obtained from Polyalthia longifolia with Anti-dyslipidemic potential: Mechanistic pathway insights exploiting co-inverse miRNA-mRNA expression analysis DOI
Nilesh Khandelwal,

Suriya P. Singh,

Prashant Rai

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177297 - 177297

Published: Jan. 1, 2025

Language: Английский

Citations

0
Understanding lncRNAs: key regulators of myogenesis and lipogenesis in farm animals DOI Creative Commons
Wenjing Liu, Mengjie Chen,

Yining Liu

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 14, 2025

Long non-coding RNAs (lncRNAs) are RNA molecules exceeding 200 nucleotides in length. Recent studies have demonstrated their involvement regulating gene expression and various biological processes. Among these, myogenesis lipogenesis particularly important because of direct effects on muscle development fat deposition farm animals. These processes crucial for determining meat quality, growth rates, overall economic value animal husbandry. Although the specific mechanisms through which lncRNAs influence these pathways still under investigation, further research into roles is optimizing breeding strategies. Here, we review characteristics lncRNAs, including biogenesis, localization, structures, with a particular focus association adipogenesis. This seeks to establish theoretical foundation enhancing production. In particular, focusing may reveal how can enhance traits animals, thereby contributing optimization

Language: Английский

Citations

0
LncIMF1 Promotes Adipogenesis of Porcine Intramuscular Preadipocyte by Sponging miR-187 DOI

Ming Feng,

Xudong Yi, Ziyi Zhang

et al.

Biochemical Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Language: Английский

Citations

0
Non-Coding RNAs in Regulating Fat Deposition in Farm Animals DOI Creative Commons
Jingxuan Li, Xueyan Zhao, Yanping Wang

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(6), P. 797 - 797

Published: March 11, 2025

Fat deposition represents a crucial feature in the expenditure of physical energy and affects meat quality farm animals. It is regulated by multiple genes regulators. Of them, non-coding RNAs (ncRNAs) play critical role modulating fat process. As well as being an important protein source, animals can be used medical models, so many researchers worldwide have explored their mechanism deposition. This article summarizes transcription factors, regulatory genes, signaling pathways involved molecular regulation process deposition; outlines progress researching roles microRNAs (miRNAs), long ncRNAs (lncRNAs), circular (circRNAs) common including pigs, cattle, sheep, ducks, chickens; identifies scientific problems field that must further investigated. has been demonstrated regulating great potential improving traits.

Language: Английский

Citations

0
Ssc-miR-130b Enhances Cell Proliferation and Represses Adipogenesis of Primary Cultured Intramuscular Preadipocytes in Pigs DOI Creative Commons

Yunqiu Yang,

Yongfang Chen,

Lijun Wang

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 375 - 375

Published: April 17, 2025

In the efforts towards germplasm innovation of livestock and poultry, strategies to improve meat quality have faced some increasingly challenging dynamic concerns. Intramuscular fat (IMF) content backfat thickness are two important traits contributing quality. MicroRNAs (miRNAs)—a class endogenous noncoding RNAs maintaining cell homeostasis by inhibiting target gene expression—have been proven as critical regulators body deposition, thus affecting farm animal production. Our previous in vitro vivo models pigs clarified that miR-130b overexpression can obviously suppress adipogenesis subcutaneous preadipocytes lower thickness. However, way regulates proliferation primary cultured porcine intramuscular (PIMPA) underlying mechanism still unknown. PIMPA derived from longissimus dorsi muscle were employed examine role further elucidate its mechanism. Lipid deposition cytoplasm was evaluated TG quantification ORO-staining, EDU-staining measure proliferation. Adipogenic proliferation-related expression conducted qPCR Western blot. MiR-130b markedly stimulated increasing cycle-related expression. Furthermore, significantly inhibited adipogenic differentiation PIMPA, mainly marker genes PPAR-γ SREBP1. addition, we proved downstream ultimately repressed adipogenesis. Ssc-miR-130b accelerated but an enhanced knowledge function ssc-miR-130b lipid providing potential implications for enhancing pork

Language: Английский

Citations

0
Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs DOI Creative Commons
Hui Feng, Tianyi Liu,

Salsabeel Yousuf

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 13, 2023

Background Long non-coding RNAs (lncRNAs) regulate adipocyte differentiation and metabolism, However, their function on subcutaneous intramuscular adipose tissues in pigs is unclear. Intramuscular fat (IMF) an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by (SCF), which severely affects the rate of pigs. It great significance porcine breeding to study mechanism lncRNA related adipogenesis lipid metabolism. Methods We identified differentially expressed lncRNAs, miRNAs mRNAs three female Laiwu deep RNA-sequencing(|log2foldchange|≥1, P _value ≤ 0.05). The gene expression profiles SCF were comparatively analyzed Bioinformatics methods identify key miRNAs, associated metabolism adipogenesis. Results A total 1209 lncRNAs (DElncRNAs), 286 (DEmiRNAs), 1597 (DEgenes) between two types adipose. Among them, 17 DElncRNAs 103 target genes play a role co-expression network, as well 59 DElncRNAs, 44 DEmiRNAs, 88 DEgenes involved ceRNA network. In GO(Gene Ontology) KEGG (Kyoto Encyclopedia Genes Genomes) pathway analysis many biological processes signaling pathways, such PPAR pathway, Wnt MAPK pathway. Conclusions By constructing ceRNAs network we found that critical regulatory accumulation TCONS_00006525, TCONS_00046551 TCONS_00000528 may WNT5A, WNT10B FDZ3 TCONS_00026517 other PPARG, RXRG SCD This provides theoretical basis further understanding post-transcriptional regulation quality formation, predicting treating diseases caused ectopic fat.

Language: Английский

Citations

10
Research progress of non-coding RNAs regulation on intramuscular adipocytes in domestic animals DOI

Yanrong Gong,

Zhongzhen Lin, Yan Wang

et al.

Gene, Journal Year: 2023, Volume and Issue: 860, P. 147226 - 147226

Published: Feb. 2, 2023

Language: Английский

Citations

8
Adipocyte Progenitor Pools Composition and Cellular Niches Affect Adipogenesis Divergence in Porcine Subcutaneous and Intramuscular Fat DOI
Xudong Yi,

Ming Feng,

Jiahua Zhu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(24), P. 14044 - 14056

Published: June 7, 2024

Intramuscular fat (IMF) contributed positively to pork quality, whereas subcutaneous (SCF) was often considered be a detrimental factor impacting growth and carcass traits. Reducing SCF while maintaining optimal IMF levels requires thorough understanding of the adipogenic differences between these two adipose depots. Our study explored in adipogenesis porcine SCF, results showed that adipocytes (SCAs) demonstrate greater potential for differentiation, both vivo vitro. Lipidomic transcriptomic analyses suggested intramuscular (IMAs) are more inclined biosynthesize unsaturated fatty acids. Furthermore, single-cell RNA sequencing (scRNA-seq) employed dissect intrinsic microenvironmental discrepancies SCF. Comparative analysis indicated enriched with preadipocytes, exhibiting an enhanced potential, characterized by higher abundance stem cells. coculture cells myogenetic niche IMAs inhibited its differentiation. Cell communication identified 160 ligand–receptor pairs channels IMF. Collectively, our elucidated novel mechanisms underpinning divergence

Language: Английский

Citations

2
Identification and Functional Prediction of Long Non-Coding RNA in Longissimus Dorsi Muscle of Queshan Black and Large White Pigs DOI Open Access
Yaqing Dou, Kunlong Qi, Yingke Liu

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(1), P. 197 - 197

Published: Jan. 12, 2023

Long non-coding RNA (lncRNA) participates in the regulation of various biological processes, but its function and characteristics intramuscular fat (IMF) deposition different breeds pigs have not been fully understood. IMF content is one important factors affecting pork quality. In present study, differentially expressed lncRNAs (DE lncRNAs) their target genes were screened by comparing Queshan Black (QS) Large White (LW) based on RNA-seq. The results displayed 55 DE between QS LW, 29 upregulated 26 downregulated, with 172 co-located genes, 6203 co-expressed genes. GO KEGG analysis showed that involved multiple pathways related to lipogenesis lipid metabolism, such as biosynthetic process, protein phosphorylation, activation MAPK activity, Jak-STAT signaling pathway. By constructing regulatory networks, lincRNA-ZFP42-ACTC1, lincRNA-AMY2-STAT1, and/or lincRNA-AMY2/miR-204/STAT1 sieved, indicate lncRNA could participate through direct or ceRNA. These findings provide a basis for analyzing molecular mechanism lay foundation developing utilizing high-quality resources local pig breeds.

Language: Английский

Citations

6