Chiral Separation and Determination of Multiple Organophosphorus Pesticide Enantiomers in Soil Based on Cellulose‐Based Chiral Column by LC–MS/MS

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(2)

Published: Feb. 1, 2025

ABSTRACT The widespread use of organophosphorus pesticides (OPs) has raised significant environmental and health concerns due to their residues in soil potential entry into the food chain. This study introduced chiral analysis methods for four OPs—methamidophos (METHP), dipterex (DIP), malathion (MALA), isothiophos‐methyl (ISOME)—using liquid chromatography–tandem mass spectrometry (LC–MS/MS) with cellulose‐based columns. Three distinct were established: one METHP, another DIP, a third MALA ISOME. Key chromatographic variables, including organic mobile phases column temperatures, systematically optimized, achieving maximum resolutions ( R s) 1.61 2.40 1.70 MALA, 2.02 QuEChERS method was employed sample pretreatment, ensuring high recoveries. All three demonstrated excellent linearity > 0.998), accuracy recoveries ranging from 79% 121%, precision RSD% < 11%, sensitivity low limits enantiomer detection (LODs) as 0.17 µg/kg 0.087 0.062 0.054 ISOME, representing improvement 16–172 times compared existing methods. Field samples Yangzizhou District, Nanchang, China, revealed contamination by concentrations single reaching up 8343 µg/kg, while exhibited varying enantiomeric ratios depth. provides robust analytical tools monitoring OP soil, contributing safety protection.

Language: Английский

Worlds apart: Plant health and One Health and a path to convergence

CABI One Health, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract Plant health is increasingly recognized as a vital part of One Health. Yet, action lags behind recognition. Historically focused on service provision, zoonotic diseases, and antimicrobial resistance, Health often overlooks plant in strategic plans. The paradox? Cross-sectoral approaches, core to Health, are already used fields like agroecology, sustainable food systems, integrated landscape management – just not under the banner. disconnect between worlds limits recognition interconnected challenges, obscures critical knowledge gaps, stifles opportunities for synergy. By staying outside Health’s framework, “plant world” misses political institutional clout bodies Quadripartite High-Level Expert Panel, essential driving global support. FAO other plant-focused stakeholders could assert their role more clearly tackling severe issues pesticide risks, mycotoxins, invasive species, biodiversity loss. Barriers change persist. professionals do associate work with traditional actors may fail see value integrating health. However, there signs that gradually happening. Ideas initiatives surfacing, demonstrating different ways which approaches can be health, such ensure all aspects problem considered looking solutions, or integrate biosecurity policy regulatory framework. planning next phase Joint Plan Action presents timely opportunity rethink strategies break down barriers currently limit true cross-domain integration. impact statement Although included definition, significant conceptual, disciplinary, power-related gaps hinder its practical integration, reinforcing persistent focus zoonoses resistance (AMR). collaboration agenda-setting entity, must address fragmentation within United Nations (UN) system coordination effective inclusion This paper adds nuance perspective debate, challenges decision-makers, researchers, practitioners funders broaden scope, recognizing achieving goals. A stakeholder process proposed build consensus definition articulated by Panel (OHHLEP): “optimizing humans, animals, plants, environment.”

Language: Английский

Is there a link between exposure to pesticides and antibiotic resistance in Gram-negative bacteria isolated from Thai farmers?

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 202, P. 107451 - 107451

Published: March 4, 2025

The organophosphate pesticides have the potential to impact microbial diversity, but their influence on antibiotic resistance (AR) in bacteria remains understudied. objective of our study was evaluate exposure acetylcholinesterase inhibitors glyphosate tolerance and AR Gram-negative isolated from digestive tracts Thai farmers. Human fecal samples Thailand, grouped by pesticide level measured blood concentration, were cultured MacConkey (McK) agar with or without 7 g/L a glyphosate-based formulation (GBF). Antibiotic susceptibility minimum inhibitory concentration (MIC) strains assessed using disk diffusion broth microdilution methods, respectively. A total 547 GNB 112 human samples. GBF medium predominantly selected Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii. MICs ranged 2 16 K. pneumoniae species harboring highest median MIC (16 g/L). rates not significantly different between exposed groups pesticides. In contrast, six mobile colistin (MCR)- and/or extended spectrum beta lactamase (ESBL)-producing E. coli pesticide-exposed group, while only one colistin-resistant strain sample which results underscore need for further research, particularly prevalence ESBL-producing strains. Additionally, we emphasize importance testing broad range better understand AR.

Language: Английский