Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of OATF against kidney stones DOI Creative Commons

S. Dai,

Qi Fang, Hongyan Li

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Introduction Orthosiphon aristatus (Blume) Miq. (OA) is widely used in folk medicine to treat kidney stones (KS). Its total flavonoids (OATF) are the primary active constituents responsible for its therapeutic effects. However, exact mechanism of action (MOA) remains unclear. This study aimed investigate pharmacological activity OATF against KS and elucidate underlying MOA. Methods Network pharmacology molecular docking were utilized predict potential targets pathways OATF. An animal model calcium oxalate crystal deposition was created using intraperitoneal injections ethylene glycol (EG) ammonium chloride (AC), alongside a human renal tubular epithelial cells (HK-2) induced by supersaturated (Ox) mechanisms oxidative stress apoptosis. The effects on damage assessed hematoxylin-eosin (H&E) periodic acid-Schiff (PAS) staining. Renal apoptosis evaluated via TUNEL MOA explored Western blotting analyses. Results analysis identified EGFR/PI3K/AKT pathway as key KS. In vitro experimental results demonstrated that effectively protected HK-2 from stress, inhibited adhesion, reduced vivo , significantly decreased serum creatinine (SCR), (Ca), phosphorus (P), blood urea nitrogen (BUN) levels CaOx-induced Sprague-Dawley (SD) rats, indicating protective Conclusion stone formation mitigated injury attenuating through activation pathway. These findings highlight OATF’s management provide scientific basis traditional use herbal medicine.

Language: Английский

GOLPH3 promotes calcium oxalate-induced renal injury and fibrosis through Golgi stress-mediated apoptosis and inflammatory responses DOI Creative Commons

Baofeng Song,

Bojun Li,

Yushi Sun

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 4, 2025

Language: Английский

Citations

0
Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of OATF against kidney stones DOI Creative Commons

S. Dai,

Qi Fang, Hongyan Li

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Introduction Orthosiphon aristatus (Blume) Miq. (OA) is widely used in folk medicine to treat kidney stones (KS). Its total flavonoids (OATF) are the primary active constituents responsible for its therapeutic effects. However, exact mechanism of action (MOA) remains unclear. This study aimed investigate pharmacological activity OATF against KS and elucidate underlying MOA. Methods Network pharmacology molecular docking were utilized predict potential targets pathways OATF. An animal model calcium oxalate crystal deposition was created using intraperitoneal injections ethylene glycol (EG) ammonium chloride (AC), alongside a human renal tubular epithelial cells (HK-2) induced by supersaturated (Ox) mechanisms oxidative stress apoptosis. The effects on damage assessed hematoxylin-eosin (H&E) periodic acid-Schiff (PAS) staining. Renal apoptosis evaluated via TUNEL MOA explored Western blotting analyses. Results analysis identified EGFR/PI3K/AKT pathway as key KS. In vitro experimental results demonstrated that effectively protected HK-2 from stress, inhibited adhesion, reduced vivo , significantly decreased serum creatinine (SCR), (Ca), phosphorus (P), blood urea nitrogen (BUN) levels CaOx-induced Sprague-Dawley (SD) rats, indicating protective Conclusion stone formation mitigated injury attenuating through activation pathway. These findings highlight OATF’s management provide scientific basis traditional use herbal medicine.

Language: Английский

Citations

0