Published: Jan. 1, 2024
Language: Английский
Published: Jan. 1, 2025
Molecular mechanism of interaction between resveratrol and lipase was studied using fluorescence spectroscopy (fluorescence spectrum, synchronous fluorescence, Three-dimensional fluorescence), ultraviolet-visible(UV-vis) spectroscopy, circular dichroic chromatography molecular docking method. The experiments (The Stern−Volmer quenching constants (Ksv)) indicated that quenched the intrinsic through static mechanism. number binding site about one. thermodynamic functions ΔG<0,ΔS<0 ΔH<0 process, which combination process spontaneous exothermic reaction Hydrogen bonds van der Waals forces effect main force them. Uv-vis spectra, spectra three-dimensional analysis showed induced changes in microenvironment around fluorophores lipase, resulting alteration spatial structure lipase. Circular contents α-helix, β-turn β-sheet were decreased of, random coil increased. All these experimental results verified reasonably explained by result. Results from this study should be useful to elucidate mechanisms contribute make full use pharmaceutical industry.
Language: Английский
Citations
0
Journal of Zoological and Botanical Gardens, Journal Year: 2025, Volume and Issue: 6(1), P. 3 - 3
Published: Jan. 13, 2025
The absence of standardized reference values for wild rodent species underscores the need comprehensive hematological and biochemical profiles. This study established robust intervals (RIs) Myocastor coypus raised in captivity, analyzing 30 nutrias (10 males, 10 females, juveniles) at a private farm northeastern Romania. Leukocyte (WBC) counts averaged 11.85 (103/µL) 10.51 11.63 juveniles, indicating consistent immune response. Hemoglobin was 11.81 g/dL 11.97 15.42 with hematocrit levels around 45%. Juveniles displayed higher MCH (38.59 pg) MCHC (38.58 g/dL), reflecting growth-related adaptations. Platelet were lower adults. Biochemical findings showed cholesterol (14.89 mg/dL) glucose (236.26 intense energy metabolism. Total proteins significantly elevated juveniles (33.17 g/dL). Creatinine uric acid adults, although calcium exceeded ranges males (12.04 mg/dL). Hepatic enzyme ALT males. These establish baseline health parameters captive nutrias, aiding monitoring improving rearing practices.
Language: Английский
Citations
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Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16
Published: April 25, 2025
Diabetic cardiomyopathy (DCM) is a common and fatal cardiac complication caused by diabetes, with its pathogenesis involving various forms of cell death mitochondrial dysfunction, particularly ferroptosis injury. Recent studies have indicated that damage play crucial roles in the onset progression DCM, though their precise regulatory mechanisms remain unclear. Of particular interest interaction between damage, as well synergistic effects, which are not fully understood. This review summarizes injury DCM explores molecular involved, an emphasis on interplay these two processes. Additionally, article offers overview targeted drugs shown to be effective cellular experiments, animal models, clinical trials, analyzing action potential side effects. The goal provide insights for future drug development applications. Moreover, challenges prospects multi-target combination therapies personalized medicine interventions practice offer strategic guidance comprehensive prevention management DCM.
Language: Английский
Citations
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Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2052 - 2052
Published: Dec. 12, 2024
Cellular rejuvenation therapies represent a transformative frontier in addressing age-related decline and extending human health span. By targeting fundamental hallmarks of aging—such as genomic instability, epigenetic alterations, mitochondrial dysfunction, cellular senescence—these aim to restore youthful functionality cells tissues, offering new hope for treating degenerative diseases. Recent advancements have showcased range strategies, including reprogramming, senolytic interventions, restoration, stem cell-based approaches, gene-editing technologies like CRISPR. Each modality has demonstrated substantial potential preclinical models is now being cautiously explored early-stage clinical trials. However, translating these from the laboratory practice presents unique challenges: safety concerns, delivery precision, complex regulatory requirements, ethical considerations, high costs impede widespread adoption. This review examines current landscape rejuvenation, highlighting key advancements, risks, strategies needed overcome hurdles.
Language: Английский
Citations
1
Drug Design Development and Therapy, Journal Year: 2024, Volume and Issue: Volume 18, P. 5641 - 5654
Published: Dec. 1, 2024
Background: Dasatinib (DASA) is associated with cardiotoxic effects, posing risks to patients. Valsartan (VAL) may offer protective benefits against these effects. This study evaluates the impact of DASA, VAL, and their combination on cardiac health. Methods: Wistar rats were treated a VAL DASA intraperitoneally every other day for 14 days. Body weight survival rates monitored. Serum levels biomarkers (CPK, LDH, AST) analyzed. Histopathological immunohistochemical analyses assessed myocardial architecture apoptosis-related protein expression. Metabolomic profiling was conducted using GC-MS identify metabolic changes across treatment groups. Results: The group experienced significant loss 50% mortality rate, while had no mortality. Cardiac like CPK, AST elevated in but significantly reduced + group. examination showed injury group, improved tissue morphology Immunohistochemical analysis revealed altered expression proteins, including caspase-3 BCL-2, compared alone. identified shifts, 15 metabolites differentiating groups, mitigated disturbances caused by DASA. Conclusion: suggesting VAL's potential therapeutic role managing DASA-induced toxicity. not only biomarker also preserved normalized profiles. These findings highlight importance integrated approaches evaluating drug efficacy suggest as promising candidate protecting function preclinical models therapy. Keywords: toxicity, dasatinib, valsartan, metabolomics, histopathology
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0