Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1263 - 1263
Published: Nov. 8, 2024
The vast, untapped potential of the world's oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as
Language: Английский
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: July 29, 2024
Soloxolone amides are semisynthetic triterpenoids that can cross the blood-brain barrier and inhibit glioblastoma growth both in vitro vivo . Here we investigate impact of these compounds on processes associated with invasiveness therapy resistance. Screening soloxolone against cells revealed ability compound 7 (soloxolone para -methylanilide) to transforming factor-beta 1 (TGF-β1)-induced glial-mesenchymal transition Compound inhibited morphological changes, wound healing, transwell migration, expression mesenchymal markers (N-cadherin, fibronectin, Slug) TGF-β1-induced U87 U118 cells, while restoring their adhesiveness. Confocal microscopy molecular docking showed reduced SMAD2/3 nuclear translocation probably by direct interaction TGF-β type I II receptors (TβRI/II). In addition, suppressed stemness as evidenced inhibition colony forming ability, spheroid growth, aldehyde dehydrogenase (ALDH) activity. Furthermore, exhibited a synergistic effect temozolomide (TMZ) cell viability. Using N-acetyl-L-cysteine (NAC) flow cytometry analysis Annexin V-FITC-, propidium iodide-, DCFDA-stained was found synergize cytotoxicity TMZ inducing ROS-dependent apoptosis. Further studies , alone or combination TMZ, effectively xenograft tumors mice. Thus, demonstrated promising potential component for glioblastoma, reducing its increasing sensitivity chemotherapy.
Language: Английский
Citations
2
Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1263 - 1263
Published: Nov. 8, 2024
The vast, untapped potential of the world's oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as
Language: Английский
Citations
0