A Non-Pharmacological Paradigm Captures the Complexity in the Mechanism of Action of Poliprotect Against Gastroesophageal Reflux Disease and Dyspepsia DOI Open Access

Sara Caterbi,

Claudio Buttarini,

Stefano Garetto

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1181 - 1181

Published: Jan. 29, 2025

When the protective mechanisms of gastroesophageal mucosa are overwhelmed by injurious factors, structural and functional mucosal integrity is compromised, resulting in a wide spectrum disorders. Poliprotect has recently been shown to be non-inferior standard-dose omeprazole for treatment endoscopy-negative patients with heartburn and/or epigastric pain or burning. Here, we provide preclinical data describing mechanism action formulation, 100% natural, biodegradable, environmental friendly medical device according EU 2017/745 containing UVCB (unknown variable composition, complex-reaction products, biological materials) substances botanical mineral origin, REACH European Chemical Agency definitions. Different vitro assays demonstrated capability adhere mucus-secreting gastric cells concomitantly deliver local barrier buffering antioxidant activity. In studies conducted accordance systems biology principles, evaluated effects this on human exposed acidic stress. Biological functions identified via Ingenuity Pathway Analysis highlighted product’s ability create microenvironment that supports integrity, promotes healing, restores balanced inflammatory status. Additionally, transepithelial electrical resistance an Ussing chamber showed preserving esophageal epithelial barriers when acid solution. Two vivo models erosive gastropathy further its topical protection against ethanol- drug-induced injury. Overall, our findings sustain feasibility paradigm shift therapeutics R&D depicting very innovative desirable mode interaction body based emerging biophysical, rather than pharmacological properties these therapeutic agents.

Language: Английский

Poliprotect®, a Medical Device Made of Substances, Potently Protects the Human Esophageal Mucosa Challenged by Multiple Agents: Evidence from In Vitro and Ex Vivo Electrophysiological Models DOI Open Access
Mohamad Khalil,

Valeria Perniola,

Elisa Lanza

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 791 - 791

Published: Jan. 18, 2025

The integrity of esophageal epithelial cells in patients with gastroesophageal reflux disease (GERD) or GERD-like symptoms is the first mechanism protection to decrease sensitivity gastric and heartburn symptoms. We investigated protective effects Poliprotect® (PPRO), a CE-marked medical device, on using vitro ex vivo models. In vitro, PPRO were tested Caco-2 cells. demonstrated safety against oxidative damage induced by hydrogen peroxide. It also preserved maintaining transepithelial electrical resistance (TEER) from calcium removal bile acid exposure (taurodeoxycholic acid, TDCA). Ex vivo, biopsies subjected endoscopy mounted Ussing chambers exposed damaging agents (HCl HCl + Untreated (control) showed significant loss (up −33%). contrast, low concentrations (50–100 µg/mL) provided strong these damages (p < 0.001), even after 60 min washing. Histological analysis confirmed barrier-enhancing effect PPRO. Overall, effectively protected epithelium both models, suggesting its potential role alleviating GERD strengthening mucosal barriers reducing reflux.

Language: Английский

Citations

1
The Esophageal Mucosa DOI
Pierfrancesco Visaggi, Evan S. Dellon

Gastrointestinal Endoscopy Clinics of North America, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Topical Protection of Esophageal Mucosa as a New Treatment of GERD DOI

Vincenzo Savarino,

Pierfrancesco Visaggi, Elisa Marabotto

et al.

Journal of Clinical Gastroenterology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Among the various factors implicated in pathogenesis of gastroesophageal reflux disease (GERD), visceral hypersensitivity and mucosal resistance have been recently re-evaluated relation to increasing phenomenon proton pump inhibitor failure, particularly patients with nonerosive (NERD). Intensive research has allowed us understand that noxious substances contained refluxate are able interact esophageal epithelium induce elicitation symptoms. The frequent evidence microscopic esophagitis increase permeability mucosa, proximity sensory afferent nerve fibers lumen favoring higher sensitivity possible activation inflammatory pathways interacting endings pathophysiological alterations confirming is impaired GERD patients. Accordingly, reinforcement protective mechanisms mucosa by topical therapies become a novel treatment target. Alginate, combination hyaluronic acid+chondroitin sulphate Poliprotect shown adhere good properties. Several placebo-controlled clinical trials these compounds, given alone or as add-on therapy for short periods, enable relieve symptoms improve quality life NERD Further studies needed confirm above results find new protectants order management

Language: Английский

Citations

0
A Non-Pharmacological Paradigm Captures the Complexity in the Mechanism of Action of Poliprotect Against Gastroesophageal Reflux Disease and Dyspepsia DOI Open Access

Sara Caterbi,

Claudio Buttarini,

Stefano Garetto

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1181 - 1181

Published: Jan. 29, 2025

When the protective mechanisms of gastroesophageal mucosa are overwhelmed by injurious factors, structural and functional mucosal integrity is compromised, resulting in a wide spectrum disorders. Poliprotect has recently been shown to be non-inferior standard-dose omeprazole for treatment endoscopy-negative patients with heartburn and/or epigastric pain or burning. Here, we provide preclinical data describing mechanism action formulation, 100% natural, biodegradable, environmental friendly medical device according EU 2017/745 containing UVCB (unknown variable composition, complex-reaction products, biological materials) substances botanical mineral origin, REACH European Chemical Agency definitions. Different vitro assays demonstrated capability adhere mucus-secreting gastric cells concomitantly deliver local barrier buffering antioxidant activity. In studies conducted accordance systems biology principles, evaluated effects this on human exposed acidic stress. Biological functions identified via Ingenuity Pathway Analysis highlighted product’s ability create microenvironment that supports integrity, promotes healing, restores balanced inflammatory status. Additionally, transepithelial electrical resistance an Ussing chamber showed preserving esophageal epithelial barriers when acid solution. Two vivo models erosive gastropathy further its topical protection against ethanol- drug-induced injury. Overall, our findings sustain feasibility paradigm shift therapeutics R&D depicting very innovative desirable mode interaction body based emerging biophysical, rather than pharmacological properties these therapeutic agents.

Language: Английский

Citations

0