Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 266, P. 155747 - 155747
Published: Nov. 28, 2024
Language: Английский
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Feb. 13, 2024
Exosomes, extracellular vesicles secreted by cells, have garnered significant attention in recent years for their remarkable therapeutic potential. These nanoscale carriers can be harnessed the targeted delivery of agents, such as pharmaceuticals, proteins, and nucleic acids, across biological barriers. This versatile attribute exosomes is a promising modality precision medicine applications, notably realm cancer therapy. However, despite substantial potential, still confront challenges tied to standardization scalability that impede practice clinical applications. Moreover, heterogeneity isolation methodologies limited cargo loading mechanisms pose obstacles ensuring consistent outcomes, thereby constraining utility. In contrast, exhibit distinct advantage diagnosis, they harbor specific signatures reflective tumor's genetic proteomic profile. characteristic endows them with potential serve valuable liquid biopsies non-invasive real-time monitoring, making possible early detection development personalized treatment strategies. this review, we provide an extensive evaluation advancements exosome research, critically examining advantages limitations context therapy diagnosis. Furthermore, present curated overview most technological innovations utilizing exosomes, focus on enhancing efficacy detection.
Language: Английский
Citations
30
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1180 - 1180
Published: Jan. 18, 2024
Oncolytic viruses (OVs) are emerging as potential treatment options for cancer. Natural and genetically engineered exhibit various antitumor mechanisms. OVs act by direct cytolysis, the potentiation of immune system through antigen release, activation inflammatory responses or indirectly interference with different types elements in tumor microenvironment, modification energy metabolism cells, antiangiogenic action. The action is pleiotropic, they show varied interactions host cells. An important impediment oncolytic virotherapy journey virus into cells possibility its binding to biological nonbiological vectors. have been demonstrated eliminate cancer that resistant standard treatments many clinical trials cancers (melanoma, lung, hepatic); however, there several resistance per se. Therefore, it necessary evaluate combination other modalities, such chemotherapy, immunotherapy, targeted therapies, cellular increase response rate. This review provides a comprehensive update on OVs, their use virotherapy, future prospects this therapy alongside therapies currently used treatment.
Language: Английский
Citations
22
Cancer Immunology Immunotherapy, Journal Year: 2024, Volume and Issue: 73(9)
Published: July 2, 2024
Recent studies have indicated that combining oncolytic viruses with CAR-T cells in therapy has shown superior anti-tumor effects, representing a promising approach. Nonetheless, the localized delivery method of intratumoral injection poses challenges for treating metastatic tumors or distal are difficult to reach. To address this obstacle, we employed HSV-1-infected cells, which systemically HSV into solid tumors. The biological function remained intact after loading them period three days. In both immunocompromised and immunocompetent GBM orthotopic mouse models, B7-H3 effectively delivered tumor lesions, resulting enhanced T-cell infiltration significantly prolonged survival mice. We also bilateral subcutaneous model observed group receiving virus exhibited significant reduction volume on injected side, while intravenous infusion carrying displayed suppressed growth sides. Hence,
Language: Английский
Citations
7
ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(4), P. 3499 - 3514
Published: Feb. 8, 2024
The global fight against Human Immunodeficiency Virus (HIV) and related viral infections stands at a pivotal juncture, demanding groundbreaking therapeutic strategies. Facing the challenges of existing antiviral treatments, such as resistance nonspecific actions, this paper unveils transformative approach. We introduce an innovative synergy between proteolysis-targeting chimeras (PROTACs) exosome-based delivery mechanisms, heralding era in combating HIV similar diseases. PROTACs emerge trailblazing solution, strategically targeting decomposing crucial proteins, thus, obstructing replication diminishing pathogenesis. Complementing this, use systems─nature's own nanoscale couriers─ensures precise effective transportation these dynamic directly to infected cells reservoirs. This synergistic strategy is not just leap forward therapy; it represents paradigm shift interventions large. path realizing full potential avant-garde technologies lies sustained research investments, cross-disciplinary collaborations, rigorous safety efficacy trials. By channeling efforts toward HIV, cornerstone health research, we are envisioning but actively forging path-breaking advancements therapeutics. more than scientific progress; beacon hope, promising significantly uplift lives those battling formidable
Language: Английский
Citations
3
Journal of Ovarian Research, Journal Year: 2024, Volume and Issue: 17(1)
Published: Aug. 5, 2024
Extracellular vesicles (EVs), particles enriched in bioactive molecules like proteins, nucleic acids, and lipids, are crucial mediators of intercellular communication play key roles various physiological pathological processes. EVs have been shown to be involved ovarian follicular function altered two prevalent gynecological disorders; polycystic syndrome (PCOS) endometriosis. Ovarian follicles complex microenvironments where folliculogenesis takes place with well-orchestrated interactions between granulosa cells, oocytes, their surrounding stromal cells. Recent research unveiled the presence EVs, including exosomes microvesicles, fluid (FFEVs), which constitutes part developing oocyte's microenvironment. In context PCOS, a multifaceted endocrine, reproductive, metabolic disorder, studies explored dysregulation these FFEVs cargo. Nine PCOS were included this review miRNAs commonly reported different studies, miR-379 miR-200, both known role female reproduction. Studies also demonstrated potential use as diagnostic tools treatment options. Endometriosis, another disorder characterized by ectopic growth endometrial-like tissue, has linked aberrant EV signaling. peritoneal women endometriosis carry that modulate immune response promote establishment maintenance lesions. derived from lesions, serum obtained patients showed no biomolecules eleven reviewed studies. Importantly, circulating biomarkers, reflecting severity pathology. Understanding interplay within human may provide valuable insights into pathophysiology Targeting EV-mediated open avenues for novel therapeutic approaches common disorders. More is essential unravel mechanisms underlying involvement its endometriosis, ultimately leading more effective personalized interventions.
Language: Английский
Citations
3
Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(10)
Published: Oct. 1, 2024
ABSTRACT The purpose of this study was to synthesize and characterize the therapeutic effect paclitaxel‐loaded polyethylene glycol modified imine‐coated iron oxide nanoparticles on cervical cancer cell line (HeLa cell). physiochemical characteristics nanocarriers were confirmed by using several methods including X‐ray diffraction (XRD), Thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT‐IR), Vibrating sample magnetometer (VSM), Dynamic light scattering (DLS), Transmission electron microscopy (TEM). results showed magnetic with size 35 nm excellent properties could load paclitaxel capacity (16%) encapsulation efficiency (80%) that has revealed pH‐dependent release behavior. anticancer effects investigated MTT assay. In comparison free drug, nanocarrier more cytotoxicity against HeLa also resulted in an increased rate apoptotic cells, assessed flow cytometry analysis. Furthermore, developed suitable hemocompatibility which can be used clinical applications.
Language: Английский
Citations
1
Cancer Immunology Immunotherapy, Journal Year: 2024, Volume and Issue: 74(1)
Published: Nov. 5, 2024
Abstract Dendritic cells (DCs) are crucial in cancer immunity, because they activate cytotoxic T by presenting tumor antigens. Recently, oncolytic virus therapy has been recognized as a systemic immune stimulator. We previously developed telomerase-specific adenovirus (OBP-301) and p53-armed OBP-301 (OBP-702), demonstrating that these viruses strongly antitumor immunity. However, their effects on DCs remained unclear. In the present study, aim was to elucidate mechanisms of DC activation OBP-702, focusing particularly tumor-derived exosomes. Exosomes (Exo53, Exo301, or Exo702) were isolated from conditioned media human murine pancreatic cell lines (Panc-1, MiaPaCa-2, PAN02) after treatment with Ad-p53, OBP-301, OBP-702. Exo702 derived Panc-1 MiaPaCa-2 significantly upregulated CD86, CD80, CD83 (markers maturation), IFN-γ vitro. Similarly, PAN02 CD86 bone marrow-derived bilateral subcutaneous model. This maturation inhibited GW4869, an inhibitor exosome release, anti-CD63, antibody targeting marker. Intratumoral injection OBP-702 into tumors increased presence mature CD8-positive draining lymph nodes, leading long-lasting through durable conclusion, exosomes play significant role following critical for abscopal effect.
Language: Английский
Citations
1
Molecules, Journal Year: 2023, Volume and Issue: 28(22), P. 7679 - 7679
Published: Nov. 20, 2023
Cancer, on a global scale, presents monumental challenge to our healthcare systems, posing significant threat human health. Despite the considerable progress we have made in diagnosis and treatment of cancer, realizing precision cancer therapy, reducing side effects, enhancing efficacy remain daunting tasks. Fortunately, emergence therapeutic viruses nanomaterials provides new possibilities for tackling these issues. Therapeutic possess ability accurately locate attack tumor cells, while serve as efficient drug carriers, delivering medication precisely tissues. The synergy two elements has led novel approach treatment—the combination nanomaterials. This advantageous overcome limitations associated with effects oncolytic insufficient tumoricidal capacity nanomedicines, enabling more effectively breach tumor’s immune barrier. It focuses lesion site even allows real-time monitoring distribution release, achieving synergistic effect. article comprehensively explores application treatment, dissecting their working mechanisms, integrating latest scientific advancements predict future development trends. approach, which combines viral therapy nanomaterials, represents an innovative effective strategy, offering perspectives field therapy.
Language: Английский
Citations
1
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 21, 2024
Abstract LHPP has been shown to be a new tumor suppressor, and tendency under-expressed in variety of cancers. Oncolytic virotheray is promising therapeutics for lung cancer recent decade years. Here we successfully constructed recombinant oncolytic adenovirus GD55-LHPP investigated the effect on growth cells vitro vivo. The results showed that had lower expression either or clinical tissues compared with normal tissues, effectively mediated cells. could inhibit proliferation cell lines rarely affected growth. Mechanically, was able induce stronger apoptosis GD55 through activation caspase signal pathway. Notably, also activated autophagy-related Further, efficiently inhibited xenograft mice prolonged animal survival rate control PBS. In conclusion, novel construct provides valuable strategy cancer-targeted therapy develop role suppress gene therapy.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: June 7, 2024
LHPP has been shown to be a new tumor suppressor, and tendency under-expressed in variety of cancers. Oncolytic virotheray is promising therapeutics for lung cancer recent decade years. Here we successfully constructed recombinant oncolytic adenovirus GD55-LHPP investigated the effect on growth cells vitro vivo. The results showed that had lower expression either or clinical tissues compared with normal tissues, effectively mediated cells. could inhibit proliferation cell lines rarely affected growth. Mechanically, was able induce stronger apoptosis GD55 through activation caspase signal pathway. Notably, also activated autophagy-related Further, efficiently inhibited xenograft mice prolonged animal survival rate control PBS. In conclusion, novel construct provides valuable strategy cancer-targeted therapy develop role suppress gene therapy.
Language: Английский
Citations
0