Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two‐step substrate addition strategy DOI Open Access

Bao Yingling,

Xueqin Wu, Xiaolong Chen

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Pterostilbene (PST), a 3’,5’‐ O ‐methylated derivative of resveratrol (RSV), is potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health‐promoting activities, such anticancer, antiviral, and antimicrobial effects. Large‐scale biosynthesis PST crucial due to the challenges associated with extracting it from plants. This study aims develop an efficient method for production using engineered Escherichia coli strain feeding RSV precursor. We introduced two‐step substrate addition strategy combined immobilized (IMRSV) on macroporous adsorption resin (MAR) enhance production. Five MARs were selected immobilization, fermentation parameters synthesis optimized. A maximum concentration 403 ± 9 mg/L was achieved, representing 239% increase over control, which one‐step free RSV. The titer reached 395 24 3‐L bioreactor. In conclusion, combination system IMRSV promising approach economical industrial‐scale PST.

Language: Английский

Recent Advances in Resveratrol Derivatives: Structural Modifications and Biological Activities DOI Creative Commons
Xiaohan Liu, Yong Pei, Jiahui Li

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 958 - 958

Published: Feb. 19, 2025

Resveratrol, a naturally occurring phenolic stilbene molecule, has been intensively researched for its anti-inflammatory, anticancer, antioxidant, antibacterial, and neuroprotective properties. However, due to limited absorption probable hepatotoxicity, it is difficult employ directly as medication, limiting therapeutic applicability. Over the last five years, numerous structural changes in resveratrol have widely studied, resulting considerable improvements pharmacological activity drug availability. This work reviews biological activities structure-activity relationships (SARs) of derivatives, with goal providing useful insights discovery new derivatives.

Language: Английский

Citations

2
Targeting the smooth muscle cell KEAP1-Nrf2-STING axis with pterostilbene attenuates abdominal aortic aneurysm DOI

Jiami Zou,

Zhihua Zheng, Weile Ye

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155696 - 155696

Published: May 10, 2024

Language: Английский

Citations

6
Serum testosterone partially mediates the adverse effects of organophosphate pesticides exposure on growth indicators of children and adolescents DOI

Zhenao Zhang,

Xiaoya Sun,

Jingyi Ren

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 294, P. 118073 - 118073

Published: March 29, 2025

Language: Английский

Citations

0
Pterostilbene Attenuates Osteoarthritis Progression Through p53-Dependent Autophagy Activation: Evidence from Network Pharmacology and Experimental Validation DOI
Jianping Wu, Chunpan Zhang, Yuanyuan Qin

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Abstract Background and Purpose: Osteoarthritis (OA) is a prevalent degenerative joint disorder characterized by cartilage deterioration pain. Autophagy dysfunction represents critical mechanism in OA pathogenesis. This study aimed to investigate the molecular which pterostilbene (PT) protects chondrocytes through autophagy activation its potential therapeutic application OA. Methods: Network pharmacology analysis identified key targets signaling pathways related PT In vitro, IL-1β-stimulated C28/I2 were used evaluate PT's effects on extracellular matrix metabolism, activity, underlying mechanisms, while p53 transcriptional inhibitor pifithrin-α verified specificity. vivo, monosodium iodoacetate-induced rat model validated efficacy. Results: 238 shared between OA, predominantly enriched autophagy-related pathways. significantly ameliorated IL-1β-induced ECM metabolic imbalance promoting cartilage-specific proteins inhibiting matrix-degrading enzymes. enhanced nuclear accumulation, activating AMPK phosphorylation, mTOR. treatment improved pathological changes (OARSI scores decreased from 14.9±0.9 7.3±0.7, P<0.001) autophagy. 3MA partially reversed protective effects. Conclusion: accumulation regulating AMPK/mTOR activate autophagy, providing evidence for as agent.

Language: Английский

Citations

0
Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two‐step substrate addition strategy DOI Open Access

Bao Yingling,

Xueqin Wu, Xiaolong Chen

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Pterostilbene (PST), a 3’,5’‐ O ‐methylated derivative of resveratrol (RSV), is potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health‐promoting activities, such anticancer, antiviral, and antimicrobial effects. Large‐scale biosynthesis PST crucial due to the challenges associated with extracting it from plants. This study aims develop an efficient method for production using engineered Escherichia coli strain feeding RSV precursor. We introduced two‐step substrate addition strategy combined immobilized (IMRSV) on macroporous adsorption resin (MAR) enhance production. Five MARs were selected immobilization, fermentation parameters synthesis optimized. A maximum concentration 403 ± 9 mg/L was achieved, representing 239% increase over control, which one‐step free RSV. The titer reached 395 24 3‐L bioreactor. In conclusion, combination system IMRSV promising approach economical industrial‐scale PST.

Language: Английский

Citations

0