LncRNA SPINT1-AS1 enhances the Warburg effect and promotes the progression of osteosarcoma via the miR-135b-5p/PGAM1 axis DOI Creative Commons
Jinyou Wang, Ning Zhang, Yan Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

Osteosarcoma (OS) is a malignant bone tumor that originates from interstitial tissues and affects the health of children adolescents. Long noncoding RNAs (lncRNAs) are an intriguing category widely distributed endogenous involved in OS progression, many which remain functionally uncharacterized. Herein, we observed increased expression lncRNA SPINT1-AS1 cell lines. Further analysis confirmed promotes proliferation metastasis cells. Moreover, miR-135b-5p was identified as downstream target SPINT1-AS1. Through bioinformatics analysis, PGAM1 mRNA validated via RIP luciferase reporter assays. could enhance by promoting aerobic glycolysis, acting ceRNA binding to miR-135b-5p, thereby increasing expression. Taken together, these results indicate functions promoter regulates progression through miR-135b-5p/PGAM1 axis.

Language: Английский

METTL14 modulates the progression and ferroptosis of colitis by regulating the stability of m6A-modified GPX4 DOI Creative Commons
Yuhua Chen, Weiguo Fan, Ying Lyu

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: Feb. 8, 2025

Ulcerative colitis (UC) is non-specific inflammatory bowel disease. UC development and progression were closely associated with epigenetic modifications. Nevertheless, the specific relationship between N6-methyladenosine (m6A) modification at RNA transcription levels pathogenesis remains unclear. We established cell models mouse through dextran sulfate sodium (DSS) induction. The expression of METTL14 analyzed via qRT-PCR western blot. In vitro functional experiments evaluated effects overexpression on viability DSS-induced NCM460 cells ferroptosis markers. Use m6A methylation detection kit, MeRIP-qPCR, stability confirmed molecular mechanism controlled by METTL14. vivo mice elucidated interaction GPX4. Findings from this study indicated a notable reduction in methyltransferase models. effectively suppressed cells. addition, enhanced GPX4 mRNA mediating modification, interplay introduced innovative therapeutic approaches for management.

Language: Английский

Citations

0
LncRNA SPINT1-AS1 enhances the Warburg effect and promotes the progression of osteosarcoma via the miR-135b-5p/PGAM1 axis DOI Creative Commons
Jinyou Wang, Ning Zhang, Yan Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

Osteosarcoma (OS) is a malignant bone tumor that originates from interstitial tissues and affects the health of children adolescents. Long noncoding RNAs (lncRNAs) are an intriguing category widely distributed endogenous involved in OS progression, many which remain functionally uncharacterized. Herein, we observed increased expression lncRNA SPINT1-AS1 cell lines. Further analysis confirmed promotes proliferation metastasis cells. Moreover, miR-135b-5p was identified as downstream target SPINT1-AS1. Through bioinformatics analysis, PGAM1 mRNA validated via RIP luciferase reporter assays. could enhance by promoting aerobic glycolysis, acting ceRNA binding to miR-135b-5p, thereby increasing expression. Taken together, these results indicate functions promoter regulates progression through miR-135b-5p/PGAM1 axis.

Language: Английский

Citations

0