In-depth investigation of microRNA-mediated cross-kingdom regulation between Asian honey bee and microsporidian DOI Open Access

Xiaoxue Fan,

Wende Zhang, Kaiyao Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: July 3, 2022

Abstract Asian honey bee Apis cerana is the original host for Nosema ceranae , a unicellular fungal parasite that causes nosemosis throughout world. Currently, interaction between A. and N. largely unknown. Here, based on our previously gained high-quality RNA-seq small data from -infected c. workers’ midguts clean spores, differentially expressed mRNAs (DEmiRNAs) in targeted by DEmiRNAs DEmRNAs microsporidian were predicted using bioinformatics, then target annotated investigated, with focus targets involved glycolysis/glyconeogenesis virulence factors as well energy mechanism immune response. It’s found 97 down-regulated (60 up-regulated) NcCKM vs NcTM1 potentially eight up-regulated (six down-regulated) miRNAs AcCKMI1 AcTMI1, 44 (15 NcTM2 putative of seven (two AcCKMI2 AcTMI2. Additionally, miR-60-y miR-676-y to up-regulate AcTMI1 genes engaged spore wall protein glycolysis/gluconeogenesis, while was glycolysis/gluconeogenesis-associated gene. Comparatively, 343 (138 AcCKM1 AcTM1 121 (112 NcCKMI NcTMI1, 247 (110 putatively 110 (104 NcTMI2. Further analysis showed 31 NcTMI1 12 AcTM1, which five immune-related pathways such phagasome Jak-STAT signaling pathway, whereas nine NcTMI2 AcCKM2 AcTM2, three including endocytosis, lysosomes, regulation autophagy. In addition, miR-21-x observed oxidative phosphorylation-related Finally, potential targeting relationship two DEmiRNAs-microsporidian pairs DEmiRNAs-host verified basis RT-qPCR. Our findings not only lay foundation exploring molecular underlying cross-kingdom workers but also offer valuable insights into bee-microsporidian interaction.

Language: Английский

MicroRNA—the Promising Molecular Tool for Engineering Stress Resistance in Crop Plants DOI

Renuka Ravinath,

Yogitha N Sagar,

Nitya Mankal

et al.

Russian Journal of Plant Physiology, Journal Year: 2024, Volume and Issue: 71(4)

Published: Aug. 1, 2024

Language: Английский

Citations

0
Influences of Edible Mushrooms on Dynamic and Diversity of Gut Microbiota DOI
Alexandru Stefan Barcan, Laura-Dorina Dinu, Mihai-Vlad Valu

et al.

Published: Jan. 1, 2023

The consumption of mushrooms impacts the immune system and gut microbiota due to presence prebiotic probiotic components found in mushrooms. Mushroom polysaccharides are bioactive macromolecules derived from fruiting bodies, mycelia, or fermentation broths edible medicinal species. Because their capacity modulate by lowering pathogen levels raising friendly microbial strains, mushroom have sparked much interest nutraceutical functional foods businesses. Bacteria can convert several inert chemicals into energy-rich, short-chain fatty acids human digestive tract. is then boosted, which aids illness prevention. This review highlights most recent studies on positive effects host via targeting bacteria. Recent polysaccharides' impact population generation summarized below. We also discuss role microbiome various disorders how certain might affect it. advocate for potential use near future as an adjuvant treatment regulating

Language: Английский

Citations

1
In-depth investigation of microRNA-mediated cross-kingdom regulation between Asian honey bee and microsporidian DOI Open Access

Xiaoxue Fan,

Wende Zhang, Kaiyao Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: July 3, 2022

Abstract Asian honey bee Apis cerana is the original host for Nosema ceranae , a unicellular fungal parasite that causes nosemosis throughout world. Currently, interaction between A. and N. largely unknown. Here, based on our previously gained high-quality RNA-seq small data from -infected c. workers’ midguts clean spores, differentially expressed mRNAs (DEmiRNAs) in targeted by DEmiRNAs DEmRNAs microsporidian were predicted using bioinformatics, then target annotated investigated, with focus targets involved glycolysis/glyconeogenesis virulence factors as well energy mechanism immune response. It’s found 97 down-regulated (60 up-regulated) NcCKM vs NcTM1 potentially eight up-regulated (six down-regulated) miRNAs AcCKMI1 AcTMI1, 44 (15 NcTM2 putative of seven (two AcCKMI2 AcTMI2. Additionally, miR-60-y miR-676-y to up-regulate AcTMI1 genes engaged spore wall protein glycolysis/gluconeogenesis, while was glycolysis/gluconeogenesis-associated gene. Comparatively, 343 (138 AcCKM1 AcTM1 121 (112 NcCKMI NcTMI1, 247 (110 putatively 110 (104 NcTMI2. Further analysis showed 31 NcTMI1 12 AcTM1, which five immune-related pathways such phagasome Jak-STAT signaling pathway, whereas nine NcTMI2 AcCKM2 AcTM2, three including endocytosis, lysosomes, regulation autophagy. In addition, miR-21-x observed oxidative phosphorylation-related Finally, potential targeting relationship two DEmiRNAs-microsporidian pairs DEmiRNAs-host verified basis RT-qPCR. Our findings not only lay foundation exploring molecular underlying cross-kingdom workers but also offer valuable insights into bee-microsporidian interaction.

Language: Английский

Citations

0