Current Opinion in Clinical Nutrition & Metabolic Care, Journal Year: 2024, Volume and Issue: 27(6), P. 527 - 528
Published: Oct. 3, 2024
Language: Английский
Journal of Biomedical Science, Journal Year: 2025, Volume and Issue: 32(1)
Published: Feb. 18, 2025
Skeletal muscle atrophy involves significant remodeling of fibers and is characterized by deficits in mitochondrial content function. These changes are intimately connected to shifts turnover, encompassing processes such as mitophagy biogenesis. However, the role these turnover remains poorly understood. We used a novel reporter model, mt-Keima mice, perform hindlimb immobilization accurately measure mitophagy. A comprehensive set analyses were conducted investigate biochemical molecular at levels. also performed image determine mitophagic flux. To further explore immobilization-induced atrophy, we treated animals with N-acetylcysteine (NAC; 150 mg/kg/day) modify reactive oxygen species (ROS) signaling colchicine (0.4 inhibit autophagy. Our study revealed that leads weakness fast-twitch (types IIA, IIX, IIB), recovery observed IIA following remobilization. This was accompanied increase Additionally, induced notable dysfunction, shown diminished respiration, increased ROS, greater whole lipid peroxidation. Treatment immobilized mice NAC enhanced respiration reduced ROS generation but suppressed flux intensified type IIX IIB fibers. administration flux, which exacerbated Colchicine treatment led reductions function, CASP9 CASP3 activation. findings emphasize limiting excessive during immobilization. Targeting may offer new strategies preserve function prolonged periods
Language: Английский
Citations
0
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16
Published: March 26, 2025
Background Chronic heart failure (CHF) is one of the leading causes high mortality worldwide. It characterized by pathological hypertrophy and poses a major threat to human health. Aconiti Lateralis Radix Praeparata widely used in ancient China treat CHF. However, pathology obscured, necessitating further exploration. Methods Prospective targets were predicted network analysis. A transverse aortic constriction (TAC) mice model was subsequently constructed determine effects aqueous extract (AEA) on The echocardiography performed investigate cardiac function. Histopathological analysis tissue conducted assess myocardial fibrosis. Nontargeted metabolomics analyze serum metabolites. phosphorylation level PI3K AKT, downstream such as Bnip3, p62, Atg5, LC3II measured Western blotting. In vitro , norepinephrine (NE) stimulate hypertrophy. Parameters reactive oxygen species levels, mitochondrial membrane potential, ATP concentration, CK/MB content detected H9c2 cells. Results AEA significantly alleviated Network indicated participation AKT CHF, modulated Praeparata. vivo administration effectively ameliorated performance, evidenced elevation ejection fraction. displayed diminishment collagen fiber. Metabolomics showed that several metabolites tetrahydroxycorticosterone, decylubiquinone taurocholic acid increased TAC serum. Additionally, levels expression Drp1, Opa1, Atg5 altered group. NE stimulation cell surface area deteriorated functions partially reversed results, mechanism associated with mitophagy. Conclusion This study revealed improved function via PI3K/AKT/Bnip3 pathway.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137393 - 137393
Published: Nov. 7, 2024
Language: Английский
Citations
1
Current Opinion in Clinical Nutrition & Metabolic Care, Journal Year: 2024, Volume and Issue: 27(6), P. 523 - 526
Published: Aug. 26, 2024
Citations
0
Current Opinion in Clinical Nutrition & Metabolic Care, Journal Year: 2024, Volume and Issue: 27(6), P. 527 - 528
Published: Oct. 3, 2024
Language: Английский
Citations
0