Bioinformatics-Based Management of Vitellogenin-like Protein’s Role in Pathogen Defense in Nicotiana tabacum L. DOI Creative Commons
Hanan Maoz, Amir Elalouf,

Amit Yaniv Rosenfeld

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(8), P. 4463 - 4463

Published: April 18, 2025

The primary objective of this study was to identify and characterize pathogen defense proteins in the Nicotiana tabacum L. proteome, focusing on their structural, functional, evolutionary properties, as well interactions with pathogen-derived molecules. Specifically, we aimed comprehensively analyze proteome pinpoint potential uncharacterized defense-related protein that has emerging roles immune responses antioxidant activity across plants animals. Through integrated computational approaches, determined relationships, structural modeling selected performed using different software, followed by validation through multiple metrics, including stereochemical checks (Ramachandran plot), MolProbity analysis, Z-scores. We further investigated functional binding regions or interaction sites. molecular docking investigate between pathogen-associated patterns (PAMPs), specifically β-glucan peptidoglycan (PGN), elucidate defensive mechanisms. Last, normal mode analysis (NMA), dynamics simulation (MDS), post-simulation analyses were employed evaluate stability mobility protein–ligand complexes. Uncharacterized vitellogenin-like (VLP: ID A0A1S4CXB2) domain chosen because its predicted immune-related features, stress response patterns, unknown role at new immunity functions. Phylogenetic revealed significant sequence homology VLPs from other members Solanaceae family. Structural showed a high-quality model, studies indicating stronger affinity for PGN (−10.16 kcal/mol) (−7.19 kcal/mol), highlighting involvement defense. NMA, MDS, exhibits more substantial extensive VLP than β-glucan. Our findings confirmed N. may function pattern recognition receptors (PRRs), capable recognizing responding pathogens activating signaling pathways. Future experimental these could plant application biotechnological approaches sustainable agriculture.

Language: Английский

Bioinformatics-Based Management of Vitellogenin-like Protein’s Role in Pathogen Defense in Nicotiana tabacum L. DOI Creative Commons
Hanan Maoz, Amir Elalouf,

Amit Yaniv Rosenfeld

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(8), P. 4463 - 4463

Published: April 18, 2025

The primary objective of this study was to identify and characterize pathogen defense proteins in the Nicotiana tabacum L. proteome, focusing on their structural, functional, evolutionary properties, as well interactions with pathogen-derived molecules. Specifically, we aimed comprehensively analyze proteome pinpoint potential uncharacterized defense-related protein that has emerging roles immune responses antioxidant activity across plants animals. Through integrated computational approaches, determined relationships, structural modeling selected performed using different software, followed by validation through multiple metrics, including stereochemical checks (Ramachandran plot), MolProbity analysis, Z-scores. We further investigated functional binding regions or interaction sites. molecular docking investigate between pathogen-associated patterns (PAMPs), specifically β-glucan peptidoglycan (PGN), elucidate defensive mechanisms. Last, normal mode analysis (NMA), dynamics simulation (MDS), post-simulation analyses were employed evaluate stability mobility protein–ligand complexes. Uncharacterized vitellogenin-like (VLP: ID A0A1S4CXB2) domain chosen because its predicted immune-related features, stress response patterns, unknown role at new immunity functions. Phylogenetic revealed significant sequence homology VLPs from other members Solanaceae family. Structural showed a high-quality model, studies indicating stronger affinity for PGN (−10.16 kcal/mol) (−7.19 kcal/mol), highlighting involvement defense. NMA, MDS, exhibits more substantial extensive VLP than β-glucan. Our findings confirmed N. may function pattern recognition receptors (PRRs), capable recognizing responding pathogens activating signaling pathways. Future experimental these could plant application biotechnological approaches sustainable agriculture.

Language: Английский

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