Optimized Hesperidin-Loaded Lipid Nanoparticles with Tea Tree Oil for Enhanced Wound Healing: Formulation, Characterization, and Evaluation
Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(3), P. 290 - 290
Published: Feb. 20, 2025
Objectives:
This
study
aimed
to
develop
hesperidin
solid
lipid
nanoparticles
(HESP-SLNs)
enhance
their
stability,
solubility,
and
sustained
release
for
wound
healing;
further
enhancement
was
achieved
through
prepared
nanostructured
carriers
(HESP-NLCs)
using
Tea
Tree
Oil
(TTO)
explore
synergistic
efficacy.
Methods:
A
factorial
design
of
24
trials
established
evaluate
the
influence
type
(X1),
conc
(%)
(X2),
surfactant
(X3),
sonication
amplitude
(X4)
HESP-SLNs
on
particle
size
(nm)
(Y1),
polydispersibility
index
(Y2),
zeta
potential
(Y3),
encapsulation
efficiency
(Y4).
The
optimized
formula
selected
utilizing
Design
Expert®
software
version
13,
which
additionally
enhanced
by
preparing
TTO-loaded
HESP-NLCs.
In
vitro
release,
Raman
spectroscopy,
transmission
electron
microscopy
were
carried
out
both
nanoparticles.
Cytotoxicity,
in
vivo
wound-healing
assessments,
skin
irritancy
tests
performed
performance
TTO-incorporated
HESP-NLCs
compared
HESP-SLNs.
Results:
demonstrated
PS
(280
±
1.35
nm),
ZP
(−39.4
0.92
mV),
PDI
(0.239
0.012),
EE%
(88.2
2.09%).
NLCs
Q6%
(95.14%)
vs.
(79.69%),
SLNs
showed
superior
antimicrobial
Both
exhibited
spherical
morphology
compatibility
between
HESP
excipients.
highest
closure
percentage,
supported
histological
analysis
inflammatory
biomarker
outcomes.
Cytotoxicity
evaluation
87%
cell
viability
untreated
HSF
cells,
test
confirmed
safety
NLCs.
Conclusions:
are
promising
candidates
exhibiting
capabilities,
making
them
a
therapeutic
option
cutaneous
management.
Language: Английский
Liposomes and Niosomes: New trends and applications in the delivery of bioactive agents for cancer therapy
International Journal of Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
unknown, P. 124994 - 124994
Published: Nov. 1, 2024
Language: Английский
Capturing the Dynamic Integrity of Carbocyanine Fluorophores-based Lipid Nanoparticles by the FRET technique
Journal of Materials Chemistry B,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Nanoparticles
capable
of
dynamically
reporting
their
structural
integrity
in
real-time
are
a
powerful
tool
to
guide
the
design
drug
delivery
technologies.
Language: Английский
Lipid Nanoparticles: Formulation, Production Methods and Characterization Protocols
Foods,
Journal Year:
2025,
Volume and Issue:
14(6), P. 973 - 973
Published: March 12, 2025
Lipid
nanoparticles
(LNs)
have
emerged
as
advanced
lipid-based
delivery
systems,
offering
an
effective
approach
for
encapsulating
and
protecting
lipid-soluble
bioactive
compounds,
increasing
their
bioavailability.
Solid
Nanoparticles
(SLNs)
Nanostructured
Carriers
(NLCs)
are
particularly
promising
compound
entrapment.
However,
to
fully
exploit
potential,
it
is
crucial
carefully
select
the
appropriate
lipid
matrices
emulsifiers.
This
review
offers
a
comprehensive,
up-to-date
examination,
considering
studies
published
in
last
15
years,
of
chemical,
physical,
structural
characteristics
lipids
employed
LN
production,
focusing
on
key
components
formulations:
matrices,
emulsifiers,
compounds.
In
addition,
provides
in-depth
analysis
production
methods,
drawing
insights
from
latest
scientific
literature,
emphasizes
most
important
characterization
techniques
LNs.
Key
parameters,
including
particle
size
(PS),
zeta
potential
(ZP),
crystallinity,
thermal
behavior,
morphology,
entrapment
efficiency
(EE),
load
capacity
(LC),
physical
stability,
discussed.
Ultimately,
this
aims
identify
critical
factors
successful
stable
LNs
that
efficiently
encapsulate
deliver
highlighting
significant
applications
food
systems.
Language: Английский
Preparation of protoporphyrin IX loaded nanostructured lipid carriers for anticancer photodynamic therapy
Journal of Biomaterials Applications,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
Photodynamic
therapy
(PDT)
is
a
promising
strategy
for
cancer
treatment.
However,
the
poor
hydrophilicity
of
most
photosensitizers
makes
them
difficult
to
enter
cells
and
also
susceptible
aggregation-induced
quenching
in
aqueous
environment.
In
this
study,
we
encapsulated
protoporphyrin
IX
(PPIX)
by
nanostructured
lipid
carrier
obtain
water-soluble
PPIX
delivery
system
(NLC-PPIX).
The
nanoparticles
exhibited
high
colloidal
stability
good
fluorescence
emission.
generation
1
O
2
from
NLC-PPIX
was
verified
using
9,10-anthracenediyl-bis(methylene)dicarboxylic
acid
(ABDA)
as
indicator.
quantum
yield
solution
calculated
be
∼9%.
flow
cytometry
imaging
confirmed
uptake
A2058
inside
under
light
excitation.
vitro
cytotoxicity
assay
showed
that
exerted
no
toxicity
on
dark
conditions,
while
irradiation
triggered
phototoxicity.
cell
viability
significantly
decreased
inhibition
rate
reached
approximately
96%
treating
with
200
μg/mL
420
nm
irradiation.
successful
PDT
effect
revealed
therapeutic
promise
our
drug
system.
Language: Английский