Phage-displayed synthetic library and screening platform for nanobody discovery DOI Open Access
Baolong Xia, Ah‐Ram Kim, Feimei Liu

et al.

Published: April 15, 2025

Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research therapeutic applications. However, traditional nanobody generation methods rely on immunization, can be costly time- consuming, restricting practical feasibility. In this study, we present a phage- displayed synthetic library discovery. To validate approach, screened nanobodies targeting various Drosophila secreted proteins. The identified were suitable applications such as immunostaining immunoblotting, supporting the phage-displayed versatile platform development. address challenge of limited accessibility to high-quality libraries, will openly available non-profit use.

Language: Английский

Utility of binding protein fusions to immunoglobulin heavy chain constant regions from mammalian and avian species DOI Creative Commons

Ningyu Zhu,

Philip M. Smallwood,

John C. Williams

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108324 - 108324

Published: Feb. 1, 2025

Language: Английский

Citations

0
CHO-S expression of a novel human recombinant IgG1 of anti-ALD antibody isolated by phage mutation display DOI
Guilin Li, Jiazhen Liu,

Zhenzhen Guan

et al.

Journal of Immunological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 113843 - 113843

Published: Feb. 1, 2025

Language: Английский

Citations

0
Advances in next-generation sequencing (NGS) applications in drug discovery and development DOI
Huihong Wang,

Jiale Huang,

Xianfu Fang

et al.

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Drug discovery is a complex and multifaceted process driven by scientific innovation advanced technologies. Next-Generation Sequencing (NGS) platforms, encompassing both short-read long-read technologies, have revolutionized the field enabling high-throughput cost-effective analysis of DNA RNA molecules. Continuous advancements in NGS-based technologies enabled their seamless integration across preclinical clinical workflows drug discovery, early-stage target identification, candidate selection, genetically stratified trials, pharmacogenetic studies. This review provides an overview current potential applications development process, including roles novel screening, medication The based on literature retrieval from PubMed Web Science databases between 2018 2024. As advance rapidly, NGS enhances accuracy generates vast datasets. These datasets are extensively integrated with other heterogeneous data systems biology mined using machine learning to extract significant insights, thereby driving progress discovery.

Language: Английский

Citations

0
Enhancing Enzyme Commission Number Prediction With Contrastive Learning and Agent Attention DOI

Wendi Zhao,

Qiaoling Han,

Fan Yang

et al.

Proteins Structure Function and Bioinformatics, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The accurate prediction of enzyme function is crucial for elucidating disease mechanisms and identifying drug targets. Nevertheless, existing commission (EC) number methods are limited by database coverage the depth sequence information mining, hindering efficiency precision annotation. Therefore, this study introduces ProteEC-CLA (Protein EC model with Contrastive Learning Agent Attention). utilizes contrastive learning to construct positive negative sample pairs, which not only enhances feature extraction but also improves utilization unlabeled data. This process helps learn differences in features, thereby enhancing its ability predict function. Integrating pre-trained protein language ESM2, generates informative embeddings deep functional correlation analysis, significantly accuracy. With incorporation Attention mechanism, ProteEC-CLA's comprehensively capture local details global features enhanced, ensuring high-accuracy predictions on complex sequences. results demonstrate that performs exceptionally well two independent representative datasets. In standard dataset, it achieves 98.92% accuracy at EC4 level. more challenging clustered split 93.34% an F1-score 94.72%. sequences as input, can accurately numbers up fourth level, annotation accuracy, makes a highly efficient precise tool enzymology research applications.

Language: Английский

Citations

0
Phage-displayed synthetic library and screening platform for nanobody discovery DOI Open Access
Baolong Xia, Ah‐Ram Kim, Feimei Liu

et al.

Published: April 15, 2025

Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research therapeutic applications. However, traditional nanobody generation methods rely on immunization, can be costly time- consuming, restricting practical feasibility. In this study, we present a phage- displayed synthetic library discovery. To validate approach, screened nanobodies targeting various Drosophila secreted proteins. The identified were suitable applications such as immunostaining immunoblotting, supporting the phage-displayed versatile platform development. address challenge of limited accessibility to high-quality libraries, will openly available non-profit use.

Language: Английский

Citations

0
Phage-displayed synthetic library and screening platform for nanobody discovery DOI Open Access
Baolong Xia, Ah‐Ram Kim, Feimei Liu

et al.

Published: April 15, 2025

Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research therapeutic applications. However, traditional nanobody generation methods rely on immunization, can be costly time- consuming, restricting practical feasibility. In this study, we present a phage- displayed synthetic library discovery. To validate approach, screened nanobodies targeting various Drosophila secreted proteins. The identified were suitable applications such as immunostaining immunoblotting, supporting the phage-displayed versatile platform development. address challenge of limited accessibility to high-quality libraries, will openly available non-profit use.

Language: Английский

Citations

0