Molecular Subtype Identification and Potential Drug Prediction Based on Anoikis-Related Genes Expression in Keratoconus

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 3 - 3

Published: Feb. 3, 2025

Anoikis is a special apoptosis accompanied by the loss of extracellular matrix (ECM) environment and decomposition ECM an important process in occurrence keratoconus (KC). This study aims to describe expression profile anoikis-related genes (ARGs) KC samples, identify differentially expressed (DEGs), characterize biological functions immune characteristics different molecular subtypes predict potential drugs based on construction co-expression network. First, we identified optimal clustering K ARGs dataset analyzed differences functional characteristics. Then weighted gene network was constructed cluster analysis obtain hub protein-protein interaction analyze nodes node-targeting drugs. By comparing between disease normal found that there were significant such as BCL2, CAV1, CEACAM5. Consistent two definite clusters basis difference. Kyoto Encyclopedia Genes Genomes Gene Ontology enrichment showed DEGs enriched significantly pathways like receptor interaction, chemokine signal, notch focal adhesion, sets proteolysis, anoikis, regulation natural killer T-cell proliferation. CIBERSORT calculation cell infiltration (monocytes plasma) molecules (CCL11, CCL14, HLA-A, HLA-B, so on). Then, phenotype, 5202 selected genes, 321 HubDEGs obtained after intersection with DEGs. Seven nodes, EIF4G1, KHSRP, PABPC1, POLR2A, PTBP1, RPS19, SMARCA4, matched or small compounds. Insulin dexamethasone target We revealed differential distinct function infiltration. The identification elucidated their therapeutic value predicted

Language: Английский

Looking to the Future of Viral Vectors in Ocular Gene Therapy: Clinical Review

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 365 - 365

Published: Feb. 5, 2025

Eye diseases can significantly affect the quality of life patients due to decreased visual acuity. Although modern ophthalmological diagnostic methods exist, some system are asymptomatic in early stages. Most seek advice from an ophthalmologist as a result rapidly progressive manifestation symptoms. A number inherited and acquired eye have only supportive treatment without eliminating etiologic factor. promising solution this problem may be gene therapy, which has proven efficacy safety shown clinical studies. By directly altering or replacing defective genes, therapeutic approach will stop well reverse progression diseases. This review examines concept therapy its application field ocular pathologies, emphasizing most recent scientific advances their potential impacts on function status.

Language: Английский