Phytomedicine, Journal Year: 2024, Volume and Issue: 136, P. 156150 - 156150
Published: Oct. 24, 2024
Language: Английский
Inflammatory Bowel Diseases, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Abstract Background Inflammatory bowel disease is a long-standing inflammatory disorder that influences the intestinal tract. The intent of this research to explore whether relative abundance Akkermansia muciniphila related IL6/STAT3 pathway and fundamental molecular mechanisms A. on trinitrobenzene sulfonic acid (TNBS)-induced enteritis mouse model, including expression cytokines proteins in signaling pathway. Methods association between was investigated by using mucosal biopsies fecal samples. TNBS-induced colitis models were performed elucidate underlying mechanisms. alteration microbiota organized 16s rRNA sequencing. Results In Crohn’s patients, level STAT3 IL-6 presented negative relationship with muciniphila. IL-6, p-STAT3, downregulated A.m+TNBS group, indicating may inhibit vivo. To investigate potential defensive role supplementation vivo enteritis, 16S sequencing analyze changes composition. results revealed marked increase microbial diversity within muciniphila-treated suggesting beneficial modulation gut microbiome associated supplementation. Conclusions Our findings declared alleviates gastrointestinal inflammation through IL-6/STAT3 This protective effect mediated downregulation STAT3, highlighting mechanism which modulates responses. work disclosed demonstrates influence against vivo, proposing it qualified as unique therapeutic focusing modulating or p-STAT3 treatment colitis.
Language: Английский
Citations
0
Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)
Published: April 22, 2025
Abstract Mounting evidence shows that gut microbiota communities and the human immune system coexist influence each other, there are a number of reports correlation between specific changes in occurrence autoimmune diseases. B lymphocytes play central role regulation both Here, we summarize microbiota-B cell pathways on diseases how cells regulate microorganisms, which provides mechanistic insights with relevance for identification potential therapeutic targets related fields.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 22, 2025
Thlaspi arvense (TA), commonly known as "Ximi" or "Subaijiang," is a traditional Chinese medicinal herb used to prevent and treat ulcerative colitis (UC). However, the precise mechanisms underlying its therapeutic effects remain unclear, necessitating further investigation identify potential pharmaceutical applications for UC management. This study aims elucidate efficacy of TA active constituents in treatment. first evaluated varying doses on 3% dextran sulfate sodium (DSS)-induced UC. Gut microbiota alterations mice were analyzed via 16S rRNA sequencing, with correlation analyses reveal relationship between gut cytokines. Then, network pharmacology was utilized identified targets Protein-protein interaction (PPI) networks, Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment employed explore TA's mechanisms. Molecular docking dynamics simulations validated interactions compounds UC-related targets. Finally, TNF pathway modulation by component, isovitexin, verified vitro vivo. alleviated DSS-induced weight loss dose-dependent manner, reduced disease activity indices, preserved intestinal mucosal barrier integrity. Subsequently, fluorescence situ hybridization (FISH) revealed suppressed microbial translocation tissues. To characterize inflammatory responses, ELISA demonstrated that modulated levels key cytokines (TNF-α, IL-1β, IL-6, IL-10) oxidative stress markers (SOD, MDA), indicating systemic anti-inflammatory effects. Building these findings, sequencing showed regulated alpha/beta diversity inhibited infectious disease-related pathways. Notably, heatmaps highlighted strong association TNF-α Escherichia-Shigella abundance, high-dose significantly reducing this pathogenic bacterial genus. systematically molecular mechanisms, 220 Consistent experimental data, PPI KEGG implicated TNF-α, AKT targets, primarily through signaling modulation. validate predictions, confirmed stable while specifically emphasized isovitexin's high affinity TNF-α. experiments vivo inhibition TNF-α-mediated NF-κB activation, studies isovitexin directly mitigated TNF-α-induced epithelial damage. Furthermore, potent activation tissues, constituent effectively cell damage, collectively highlighting their complementary Collectively, (TA) ameliorates synergistic involving modulation, suppression, preservation. By remodeling communities reduce colonization translocation. concurrently inhibits TNF-α/NF-κB-driven inflammation, regulation. attenuates demonstrating multi-scale efficacy. These findings establish multi-target spanning host-microbe intracellular signaling, providing rationale standardizing TA-based formulations advancing precision agent bowel diseases.
Language: Английский
Citations
0
Medical Oncology, Journal Year: 2025, Volume and Issue: 42(6)
Published: May 5, 2025
Language: Английский
Citations
0
Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)
Published: May 10, 2025
Administering beneficial bacteria as probiotics to restore the intestinal microbiota and its metabolic functions, such butyrogenesis, is a promising treatment strategy in ulcerative colitis (UC). This study aimed investigate effect of combination probiotics, consisting lactic acid bacterium Weizmannia coagulans SANK70258 lactate-utilizing butyrate-producing Anaerostipes caccae or Clostridium butyricum, on colonic environment using an vitro culture model with fecal inoculums from seven patients UC. Co-inoculated W. A. neither inhibited each other's growth nor significantly affected relative abundance other bacterial species; however, was when co-inoculated C. butyricum. The pro-inflammatory (Escherichia sp. unclassified Enterobacteriaceae) Bifidobacterium spp. decreased coagulans-C. butyricum cultures. Inoculation any alone did not increase butyrate production, whereas co-inoculation increased levels. Overall, results suggested that have synergistic effects through cross-feeding can effectively butyrogenesis KEY POINTS: • Effects were evaluated UC colon. producers effects. Co-inoculation enhanced butyrogenesis.
Language: Английский
Citations
0
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: May 19, 2025
Short-chain fatty acids (SCFAs), the primary metabolites produced by microbial fermentation of dietary fibers in gut, have a key role protecting gut health. Increasing evidence indicates SCFAs can exert effects on distant tissues and organs beyond via blood circulation. Osteoarthritis (OA) is chronic inflammatory joint disease that severely diminishes physical function quality life. However, effective clinical treatments for OA remain elusive. Recent studies shown beneficial damaged joints OA. mitigate progression preserving intestinal barrier maintaining integrity cartilage subchondral bone, suggesting they substantial potential to be adjunctive treatment strategy This review described human body their cellular signaling mechanism, summarized multiple (especially butyrate, propionate, acetate) prevention regulating gut-joint axis, providing novel insights into promising applications.
Language: Английский
Citations
0
Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(5)
Published: May 1, 2025
Abstract Background The multidimensional regulatory mechanism of the gut–brain–immune axis in context inflammatory bowel disease (IBD) has garnered significant attention, particularly regarding how intestinal microbiota finely regulates immune responses through cells and sensory neurons. Main Body Metabolites produced by influence phenotype switching via complex signalling pathways, thereby modulating their anti‐inflammatory pro‐inflammatory functions during inflammation. Furthermore, neurons exhibit heightened sensitivity to microbial‐derived signals, which is essential for preserving balance controlling pathological inflammation integrating peripheral environmental signals with local responses. dynamic equilibrium between neuroimmunoregulation mediated collectively sustains homeostasis within intestine. However, this coordination markedly disrupted under conditions associated IBD. Conclusion An in‐depth exploration interactions among cells, gut may yield insights into mechanisms underlying IBD guide creation new treatment approaches. Key points gut‐brain‐immune axis, neuroimmune Microbiota‐derived metabolites affecting Neurons secrete mediators, enabling bidirectional communication homeostasis. Disruptions contribute IBD, offering therapeutic targets.
Language: Английский
Citations
0
Food & Function, Journal Year: 2024, Volume and Issue: 15(17), P. 8700 - 8711
Published: Jan. 1, 2024
A schematic diagram illustrates how OPO protects against DSS-induced colitis by modulating gut microbiota, enhancing intestinal epithelial barrier integrity, and subsequently downregulating the TLR4-MyD88-NF-κB signaling pathway.
Language: Английский
Citations
3
Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 123072 - 123072
Published: Sept. 1, 2024
Language: Английский
Citations
3
Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3183 - 3183
Published: Oct. 7, 2024
This study was to investigate the effects of polysaccharides (PPM60-III) and sulfated (SPPM60-III) pine pollen on Th17/Treg balance, inflammatory cytokines, intestinal microbiota, metabolite distribution in 3% DSS drinking water-induced UC mice. First all, physiological results showed that PPM60-III SPPM60-III could alleviate UC, which shown by reduction liver Treg cells, rebalance Th17/Treg, modulation cytokines. In addition, 16S rRNA decrease
Language: Английский
Citations
3