Gut microbiota and metabolic responses to a 12-week caloric restriction combined with strength and HIIT training in patients with obesity: a randomized trial DOI Creative Commons
Libuša Nechalová, Viktor Bielik, Ivan Hric

et al.

BMC Sports Science Medicine and Rehabilitation, Journal Year: 2024, Volume and Issue: 16(1)

Published: Dec. 5, 2024

Nowadays, obesity has become a major health issue. In addition to negatively affecting body composition and metabolic health, recent evidence shows unfavorable shifts in gut microbiota individuals with obesity. However, the effects of weight loss on microbes metabolites remain controversial. Therefore, purpose this study was investigate 12-week program patients We conducted controlled trial 23 male female Twelve participants completed caloric restriction combined strength HIIT training (INT, pre-BMI 37.33 ± 6.57 kg/m2), eleven were designated as non-intervention controls (pre-BMI 38.65 8.07 kg/m2). Metagenomic sequencing V3-V4 region 16S rDNA gene from fecal samples allowed for classification. Nuclear magnetic resonance spectroscopy characterized selected serum metabolite concentrations. Within INT, we observed significant improvement composition; decrease liver enzymes (AST, ALT, GMT); increase relative abundance commensal bacteria (e.g., Akkermansia muciniphila, Parabacteroides merdae, Phocaeicola vulgatus); SCFA-producing genera Butyrivibrio, Coprococcus, Blautia). addition, correlations found between microbes, composition, biomarkers, SCFAs. Notably, Random Forest Machine Learning analysis identified predictors (Butyrivibrio fibrisolvens, Blautia caecimuris, Coprococcus comes, waist circumference) moderate ability discriminate INT subjects pre- post-intervention. Our results indicate that positively influences microbiota, microbial metabolites, demonstrating among these variables. linked obesity, e.g., muciniphila. Additionally, our contributes ongoing debate about role SCFAs SCFA producers after program. The registered [05/12/2014] ClinicalTrials.gov (No: NCT02325804).

Language: Английский

Metabolic-Dysfunction-Associated Steatotic Liver Disease: Molecular Mechanisms, Clinical Implications, and Emerging Therapeutic Strategies DOI Open Access

Jeysson E. Mejía-Guzmán,

Ramón A. Belmont-Hernández,

Norberto C. Chávez‐Tapia

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2959 - 2959

Published: March 25, 2025

Metabolic-dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty (NAFLD), is a highly prevalent metabolic disorder characterized by hepatic steatosis in conjunction with at least one cardiometabolic risk factor, such obesity, type 2 diabetes, hypertension, or dyslipidemia. As global rates of obesity and syndrome continue to rise, MASLD becoming major public health concern, projections indicating substantial increase prevalence over the coming decades. The spectrum ranges from simple metabolic-dysfunction-associated steatohepatitis (MASH), fibrosis, cirrhosis, hepatocellular carcinoma, contributing significant morbidity mortality worldwide. This review delves into molecular mechanisms driving pathogenesis, including dysregulation lipid metabolism, chronic inflammation, oxidative stress, mitochondrial dysfunction, gut microbiota alterations. Recent advances research have highlighted role genetic epigenetic factors progression, well novel therapeutic targets peroxisome proliferator-activated receptors (PPARs), fibroblast growth factors, thyroid hormone receptor beta agonists. Given multifaceted nature MASLD, multidisciplinary approach integrating early diagnosis, insights, lifestyle interventions, personalized therapies critical. underscores urgent need for continued innovative treatment strategies precision medicine approaches halt progression improve patient outcomes.

Language: Английский

Citations

1
The Probiotic Yeast, Milmed, Promotes Autophagy and Antioxidant Pathways in BV-2 Microglia Cells and C. elegans DOI Creative Commons
Federica Armeli, Beatrice Mengoni, Emily Schifano

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 393 - 393

Published: March 27, 2025

Background: Autophagy, a catabolic process essential for maintaining cellular homeostasis, declines with age and unhealthy lifestyles, contributing to neurodegenerative diseases. Probiotics, including Milmed yeast, have demonstrated anti-inflammatory antioxidant properties. This study evaluated the activity of on BV-2 microglial cells in vitro vivo model Caenorhabditis elegans (C. elegans) restoring autophagic processes. Methods: were incubated S. cerevisiae (Milmed treated yeast or untreated yeast) then stimulated lipopolysaccharide (LPS). mRNAs factors enzymes assessed by qPCR; mTOR NRF2 ELISA. pNRF2 compared cytosolic was immunofluorescence. The longevity, body size, reactive oxygen species (ROS) levels C. measured fluorescence microscopy. Results: Treatment YPD cultured dried powder obtained from it promoted flux, as shown increased expression Beclin-1, ATG7, LC3, p62 inhibition mTOR, It also enhanced response increasing NRF2, SOD1, GPX; moreover, enhanced, dietary supplementation prolonged survival reduced age-related ROS accumulation without changing gst-4. pro-longevity effect found be dependent SKN-1/Nrf2 activation, absence benefit skn-1 mutants. Conclusions: demonstrates significant pro-autophagy effects elegans, thereby extending lifespan improving stress resistance, which, together previously activity, highlights its role highly effective probiotic beneficial health effects. Activation SKN-1/NRF2 pathway modulation autophagy support therapeutic potential neuroprotection healthy aging.

Language: Английский

Citations

1
Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals DOI Creative Commons

Manlan Guo,

Xiaofei Jiang, Hui Ouyang

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Bifidobacterium bifidum 1007478 derived indole-3-lactic acid alleviates NASH via an aromatic hydrocarbon receptor-dependent pathway in zebrafish DOI

Chao Tian,

Shizhou Deng, Zhao Zhang

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123557 - 123557

Published: March 1, 2025

Language: Английский

Citations

0
Poncirus trifoliata Extract and Its Active Coumarins Alleviate Dexamethasone‐Induced Skeletal Muscle Atrophy by Regulating Protein Synthesis, Mitochondrial Biogenesis, and Gut Microbiota DOI Open Access

Hyejin Ko,

Tam Thi Le,

Ngoc Bao Nguyen

et al.

Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

ABSTRACT Sarcopenia, an age‐related decline in skeletal muscle mass and function, contributes to frailty increased morbidity the elderly. This necessitates development of effective interventions combat atrophy. study investigated therapeutic potential Poncirus trifoliata ethanol extract (PT) its coumarin derivatives against dexamethasone (DEX)‐induced We employed vitro vivo models DEX‐induced C2C12 myotubes were used for mechanistic studies. C57BL/6J mice received DEX injections oral PT supplementation (50 mg/kg/day) evaluate effects on mass, gene expression, gut microbiota composition. In vitro, enhanced protein synthesis, mitochondrial biogenesis, myogenic differentiation DEX‐exposed myotubes, with auraptene, ponciol, triphasiol identified as key bioactive coumarins. vivo, significantly attenuated atrophy, increasing tibialis anterior by 36% ( p < 0.01), grip strength 31% 0.001), maximal running speed 18% 0.05). Mechanistically, upregulated genes associated function health. Furthermore, modulated composition, notably Phocaeicola vulgatus abundance 2.2‐fold, which correlated improved performance R = 0.58, 0.01). These findings suggest that derivatives, particularly triphasiol, hold promise agents combating The observed benefits may be mediated through modulation gut‐muscle axis.

Language: Английский

Citations

0
Positive nutritional selection of adults with healthy lifestyle and high daily fiber consumption for the isolation of beneficial intestinal bacteria: the iTARGET cohort study protocol. DOI Creative Commons
A. Caillé,

Chloé Connan,

Noelle Lyon Belgy

et al.

MethodsX, Journal Year: 2025, Volume and Issue: unknown, P. 103268 - 103268

Published: March 1, 2025

Recent advances in the study of gut microbiota has pointed to its under-utilized source potentially beneficial bacteria, known as next generation probiotics, offering a promising avenue restore or compensate impaired toward healthy state. Aside from difficulties achieve in-lab adequate culture conditions, use bacterial isolates is also limited by their bioavailability donor itself. In iTARGET study, we positively selected donors based on diet enriched fiber, that been shown increase prevalence species associated with health. The monocenter, prospective, observational adults lifestyle and high daily fiber consumption. We aim recruit individuals two phases, first one for all will permit identification carriers bacteria interest second phase subset allow isolation previously identified bacteria. Our primary outcome at least isolate. secondary outcomes comprised throughput metagenomic profiles intestinal characterization cultured isolates. was approved French Research Ethics Committees (Comité de Protection des Personnes Sud-Est I) under National reference ID 2023-A01677-38. Study findings results be published peer-reviewed Open Access journals. (Trial registration number ClinicalTrials.gov: NCT06166810).

Language: Английский

Citations

0
Full-length 16S rRNA sequencing revealed an altered microbiome diversity and composition of the jejunum and cecum in chicken infected with Eimeria necatrix DOI

Nian-Yu Xue,

Qianqian Feng, Yu Zhu

et al.

Veterinary Parasitology, Journal Year: 2025, Volume and Issue: unknown, P. 110458 - 110458

Published: March 1, 2025

Language: Английский

Citations

0
Lactobacillus johnsonii-derived leucic acid promotes fatty acid absorption and deposition by targeting CD36 DOI
Yunxia Li, Zhiming Zhang, Qi Han

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Language: Английский

Citations

0
The Microbiome and Metabolic Dysfunction-Associated Steatotic Liver Disease DOI Open Access
Diren Beyoğlu, Jeffrey R. Idle

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2882 - 2882

Published: March 22, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a condition wherein excessive fat accumulates in the liver, leading to inflammation and potential damage. In this narrative review, we evaluate tissue microbiota, how they arise their constituent microbes, role of intestinal hepatic microbiota MASLD. The history bacteriophages (phages) occurrence part causation MASLD, conversely, "phage therapy" for antibiotic resistance, obesity, are all described. metabolism bile acids dietary tryptophan histidine defined, together with impacts individual metabolites on MASLD pathogenesis. Both periodontitis dysbiosis may cause microorganisms involved these processes discussed. Novel treatment opportunities involving exist include fecal transplantation, probiotics, prebiotics, synbiotics, supplements, intermittent fasting, phages or holins endolysins. Although FDA yet approve phage therapy clinical use, there multiple FDA-approved trials, represent new horizon future

Language: Английский

Citations

0
Potential Probiotics for the Therapy of Metabolic Dysfunction-Associated Steatotic Liver Disease DOI Creative Commons

Xiayun Li,

Liyan Yu,

Zong‐Gen Peng

et al.

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD), a prevalent metabolic disorder globally, has presented an urgent need for effective therapeutic agents. The complex pathogenesis of MASLD and the failure numerous clinical trials targeting this have prompted exploration probiotic intervention as novel strategy. Emerging evidence underscores pivotal role gut-liver axis in progression, particularly through dysregulation gut microbiota composition, impaired intestinal barrier integrity, aberrant bacterial metabolite signaling. Preclinical studies indicate that specific strains may ameliorate by restoring microbial homeostasis, fortifying function, attenuating hepatic inflammation via crosstalk. Preliminary further support beneficial effects probiotics reducing steatosis improving parameters. Future research should prioritize elucidating strain-specific mechanisms, optimizing formulations, addressing challenges related to long-term efficacy, safety, personalized regimens. This review comprehensively evaluates current on applications treatment highlights critical directions advancing translational field.

Language: Английский

Citations

0