Current Understanding of Cardiovascular Calcification in Patients with Chronic Kidney Disease DOI Open Access
Sijie Chen, Ri‐Ning Tang, Bi-Cheng Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10225 - 10225

Published: Sept. 23, 2024

The burden of chronic kidney disease (CKD) is increasing, posing a serious threat to human health. Cardiovascular calcification (CVC) one the most common manifestations CKD, which significantly influences morbidity and mortality patients. manifestation CVC an unusual accumulation mineral substances containing calcium phosphate. main component hydroxyapatite. Many cells are involved in this process, such as smooth muscle (SMCs) endothelial cells. osteogenic process initiated by complex mechanisms metabolic disorders phosphorus minerals, inflammation, extracellular vesicles, autophagy, micro-RNAs with variety signaling pathways like Notch, STAT, JAK. Although drug therapy dialysis technology continue advance, survival time quality life patients still face challenges. Therefore, early diagnosis prevention CKD-related CVC, reducing its rate, improving patients’ have become urgent issues field public In review, we try summarize state-of-the-art understanding progression hope that it will help treatment CKD.

Language: Английский

Inhibition of colorectal cancer in Alzheimer’s disease is mediated by gut microbiota via induction of inflammatory tolerance DOI Creative Commons
Nan Zhang, Rui Zhang, Lei Jiang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(37)

Published: Sept. 3, 2024

Epidemiological studies have revealed an inverse relationship between the incidence of Alzheimer’s disease (AD) and various cancers, including colorectal cancer (CRC). We aimed to determine whether CRC is reduced in AD-like mice gut microbiota confers resistance tumorigenesis through inducing inflammatory tolerance using 16S ribosomal RNA gene sequencing fecal transplantation (FMT). experienced a significantly decreased induced by azoxymethane–dextran sodium sulfate as evidenced suppressed intestinal inflammation compared with control mice. However, FMT from age-matched reversed inhibitory effects on response The key bacterial genera microbiota, Prevotella , were increased both patients amnestic mild cognitive impairment (aMCI) but CRC. Pretreatment low-dose -derived lipopolysaccharides (LPS) vivo vitro inhibited Imbalanced barrier permeability, which facilitated LPS absorption into blood, causing decline aMCI. These data reveal that exerts resistant effect via presence AD. findings provide biological evidence demonstrating AD

Language: Английский

Citations

5
Runx2-NLRP3 Axis Orchestrates Matrix Stiffness-evoked Vascular Smooth Muscle Cell Inflammation DOI
Zhiqing Li, Hao Wu, Fang Yao

et al.

AJP Cell Physiology, Journal Year: 2025, Volume and Issue: 328(2), P. C467 - C482

Published: Jan. 7, 2025

As a hallmark of chronic kidney disease (CKD), arterial stiffening is related to increased vascular inflammation and cardiovascular morbidity, whereas the underlying mechanism unclear. The study demonstrates that stiffness precedes onset inflammation, matrix stimulates transdifferentiation smooth muscle cells (VSMCs) an inflammatory phenotype via activating Runx2-NLRP3 signaling, which provides novel insights into CKD-related disorder treatment.

Language: Английский

Citations

0
Regulation of pyroptosis by NF-κB signaling DOI Creative Commons
Ling Yang, Yan Zhang,

Zhuodong Chai

et al.

Frontiers in Cell Death, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 7, 2025

Pyroptosis is a form of proinflammatory cell death characterized by inflammasome activation, pore formation, and the release pro-inflammatory cytokines such as interleukin-1β (IL-1β) IL-18 upon rupture. Nuclear factor-κB (NF-κB), prototypical transcription factor, plays critical role in immune system regulation. Recent research highlights multifaceted roles NF-κB signaling pyroptosis. Various immunologically relevant ligands their receptors can activate pathway to promote pyroptosis, with Toll-like (TLRs), IL-1 (IL-1Rs), TNF (TNFRs) being most prominent. regulates key components inflammasomes involved particularly NLRP3 inflammasome. studies also indicate that modulates activation NLRC4 AIM2 through distinct pathways diverse inflammatory conditions, acute lung injury neuroinflammation. Additionally, mediates production cytokines, including IL-1β, IL-33, TNF-α, which further regulate This review examines recent advances understanding regulating pyroptosis during infection inflammation.

Language: Английский

Citations

0
Higher serum Metrnl levels are associated with increased vascular calcification in hemodialysis patients DOI Creative Commons
C Liu, Wenwen Ren, Aihua Zhang

et al.

Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)

Published: Jan. 22, 2025

Background Meteorin-like (Metrnl) is a newly identified secreted protein that plays important roles in metabolic and inflammation-related diseases. Our study aimed to evaluate serum Metrnl levels establish their relationship with vascular calcification (VC) hemodialysis (HD) patients.

Language: Английский

Citations

0
Lactobacillus johnsonii Generates Cyclo(pro‐trp) and Promotes Intestinal Ca2+ Absorption to Alleviate CKD–SHPT DOI Creative Commons
Xubin Zeng, Lihua Sun, Huichao Xie

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Patients with chronic kidney disease (CKD) are at a high risk of developing secondary hyperparathyroidism (SHPT), which may cause organ dysfunction and increase patient mortality. The main clinical interventions for CKD–SHPT involve calcium supplements to boost absorption, but ineffective some patients, the reasons remain unclear. Here, CKD mice divided into low groups based on intact parathyroid hormone (iPTH) levels. group exhibits significant changes in gut microbes, including decrease Lactobacillus , an hyperplasia, intestinal calcium. Fecal microbiota transplantation L. johnsonii colonization indicate link between microbes CKD–SHPT. Clinically, higher levels correlated milder receiver operating characteristic (ROC) curve abundance surgical is 0.81, calibration confirming predictive accuracy, decision analysis revealing good applicability. In vivo vitro experiments show that cyclo(pro‐trp) enhance inflow lower iPTH epithelial cells via calcium‐sensing receptor transient potential vanilloid 4 pathways. This study identified crucial role CKD–SHPT, unveiling new mechanism imbalance offering novel strategies SHPT treatment drug development.

Language: Английский

Citations

0
Different doses of hydroxychloroquine regulate the structure of intestinal flora and glycosyltransferase activity in rats with IgA nephropathy DOI Creative Commons
Lingling Bao, Xueyan Bian,

Liling Ren

et al.

Immunobiology, Journal Year: 2025, Volume and Issue: unknown, P. 152891 - 152891

Published: March 1, 2025

Hydroxychloroquine (HCQ), by virtue of its ability to reduce proteinuria, is an alternative therapy for Immunoglobulin A nephropathy (IgAN). This study investigated the effects different doses HCQ on structure intestinal flora and glycosyltransferase activity in IgAN rats. model rats constructed treatment bovine serum albumin, castor oil lipopolysaccharide were administered with (18 or 36 mg/kg) gavage. Then number urine erythrocyte renal function evaluated. The levels galactose-deficient IgA1 (Gd-IgA1), B cell activation factor (BAFF) C-reactive protein (CRP) those inflammatory factors tissue detected ELISA. Renal injury IgA deposition assessed histological analysis. expressions Core 1 beta1,3-galactosyltransferase (C1GALT1), synthase specific molecular chaperone (COSMC) ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 (ST6GALNAC2) quantified qRT-PCR, Western blot vitro enzyme assays. 16 s rDNA sequencing was used analyze dose-dependently decreased creatinine, UREA, Gd-IgA1, BAFF, CRP protein, waned erythrocyte, inhibited tissue, up-regulated C1GALT1, COSMC down-regulated ST6GALNAC2 expression peripheral blood mononuclear cells (PBMCs). could glomerular swelling mesangial area improve imbalance

Language: Английский

Citations

0
Blood metabolites mediate causal inference studies on the effect of gut microbiota on the risk of vascular calcification DOI Creative Commons
Aoran Huang,

Jianshuai Ma,

Huijin Zhu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Honokiol attenuates oxidative stress and vascular calcification via the upregulation of heme oxygenase-1 in chronic kidney disease DOI

Xuemin Xian,

Xin Zhao, Xingchen Zhou

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117318 - 117318

Published: April 1, 2025

Language: Английский

Citations

0
Segatella copri Outer-Membrane Vesicles Are Internalized by Human Macrophages and Promote a Pro-Inflammatory Profile DOI Open Access

Alison Sepúlveda-Pontigo,

Karissa Chávez-Villacreses,

Cristóbal Madrid-Muñoz

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3630 - 3630

Published: April 11, 2025

Increased abundance of Segatella copri (S. copri) within the gut microbiota is associated with systemic inflammatory diseases, including rheumatoid arthritis. Although outer-membrane vesicles (OMVs) Gram-negative bacteria are important players in microbiota-host communication, effect S. copri-derived OMVs on immune cells unknown. Macrophages engulf and eliminate foreign material conditioned by environmental signals to promote either homeostasis or inflammation. Thus, we aimed explore impact copri-OMVs human macrophages vitro, employing THP-1 monocyte-derived macrophage models. The uptake DiO-labeled into was monitored confocal microscopy flow cytometry. Furthermore, phenotype cytokine secretion naïve (M0), pro-inflammatory (M1), anti-inflammatory (M2) analyzed cytometry ELISA, respectively. We show that enter through macropinocytosis clathrin-dependent mechanisms. copri-OMVs, but not parental bacterium, induced a dose-dependent increase expression M1-related surface markers M0 M2 activated large amounts cytokines M1 macrophages. These results highlight an role promoting responses, which might contribute diseases.

Language: Английский

Citations

0
Qilian Jiechang Ning Alleviates TNBS-Induced Ulcerative Colitis in Mice and Segatella copri Outer Membrane Vesicle-Triggered Inflammation in Colon Epithelial Cells via the Caspase-1/11-GSDMD Pathways DOI Creative Commons
Jinyang Hu, Junjie Niu, Shisheng Jiang

et al.

Journal of Innate Immunity, Journal Year: 2025, Volume and Issue: 17(1), P. 262 - 276

Published: May 14, 2025

Introduction: Qilian Jiechang Ning (QJN), a traditional Chinese herbal formula, has demonstrated potential therapeutic effects in the treatment of ulcerative colitis (UC). This study aims to investigate mechanism QJN outer membrane vesicles (OMVs) Segatella copri (S. copri)-induced colon epithelial cells and UC mice. Methods: Transmission electron microscopy (TEM) nanoparticle tracking analysis (NTA) were utilized assess morphology size OMVs. Inflammation markers tight junction protein levels HCoEpiCs induced by OMVs monitored using ELISA western blot. was administered intervene treated with S. Additionally, trinitrobenzene sulfonic acid (TNBS)-induced mouse models conducted evaluate on UC. Results: significant reduction particle size, concentration, LPS content. In HCoEpiCs, effectively decreased expression inflammation-inducing cytokines (IL-1β, IL-18, IL-6, TNF-α) proinflammatory proteins (GSDMD-N, NLRP3, ASC, cleaved Caspase-1, Caspase-4) triggered OMVs, while enhancing (ZO-1 Occludin). models, significantly reduced Disease Activity Index (DAI), improved length, lowered levels, ameliorated colonic tissue damage, inhibited Caspase-1- Caspase-11-dependent inflammatory responses. Conclusion: can alleviate copri-OMV-induced response reduce symptoms modulating Caspase-1 Caspase-11 pathways.

Language: Английский

Citations

0