SAA1 released by tumor cells facilitates the aggressiveness of breast cancer by inducing reprogramming of molecular phenotypes in immune cells DOI Creative Commons

Jinkun Xia

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract Most breast cancer patients are diagnosed at an advanced stage and have a poor prognosis. Recurrence of tumor metastasis major obstacles to clinical treatment. It is imperative explore new diagnostic prognostic markers improve the early diagnosis outcomes cancer. Recently, metastatic cancers transcriptional signature reveals Serum amyloid A1 (SAA1), acute-phase apolipoprotein reactant, associated with in expression clinicopathological features. However, its regulatory function remains elusive, contribution uncertain. In this research, we downloaded mRNA-sequencing data from Gene Expression Omnibus (GEO) database (GSE102818, GSE28785, GSE134591) comprehensively investigate relationship between SAA1 impact on implications, further unveiled connection SAA1-mediated immunoregulation We found that implicated cell migration regulation immune cells by modulating cytokine-cytokine receptor interaction. Meanwhile, released was demonstrated contribute inducing adipocytes reprogramming. Several current viewpoints propose reprogramming molecular phenotype driver invasion microenvironment. Based previous studies our findings, hypothesized cellular may also apply microenvironment, interaction through release relevant aggressiveness cancer, which help patient decision-making for immunotherapy.

Language: Английский

The molecular pathogenesis of SOX2 in prostate cancer DOI Creative Commons
Shixue Liu, Honglian Yu, Zhankui Zhao

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 20, 2025

SOX2 is one of the members SOX transcription factor family, which believed to be an important that plays a role in embryonic development, maintenance stem cells, cancer progression, and resistance treatment. There increasing evidence suggesting crucial for initiation, invasion, metastasis, treatment prostate cancer, therefore understanding mechanism can provide better targets cancer. This article reviews structural domains, normal physiological functions, progression SOX2, providing potential

Language: Английский

Citations

0
WGCNA-ML-MR integration: uncovering immune-related genes in prostate cancer DOI Creative Commons

Jing Lv,

Yuhua Zhou,

Shengkai Jin

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: April 7, 2025

Prostate cancer is one of the most common tumors in men, with its incidence and mortality rates continuing to rise year by year. Prostate-specific antigen (PSA) commonly used screening indicator, but lack specificity leads overdiagnosis overtreatment. Therefore, identifying new biomarkers related prostate crucial for early diagnosis treatment cancer. This study utilized datasets from Gene Expression Omnibus (GEO) screen differentially expressed genes (DEGs) employed Weighted Co-expression Network Analysis (WGCNA) identify driver highly associated within modules. The intersection was taken, Kyoto Encyclopedia Genes Genomes (KEGG) Ontology (GO) enrichment analyses were performed. Furthermore, a machine learning algorithm core construct diagnostic model, which then validated an external validation dataset. correlation between immune cell infiltration analyzed, Mendelian randomization (MR) analysis conducted closely identified six biomarkers: SLC14A1, ARHGEF38, NEFH, MSMB, KRT23, KRT15. MR demonstrated that MSMB may be important protective factor In q-PCR experiments on tumor tissues adjacent non-cancerous patients, it found that: compared tissues, expression level ARHGEF38 significantly increased, while levels KRT15 decreased. To further validate these findings at protein level, we Western blot analysis, corroborated results, demonstrating consistent patterns all biomarkers. IHC results confirmed markedly reduced. Our reveals are potential cancer, among play role

Language: Английский

Citations

0
Multifaceted roles of OCT4 in tumor microenvironment: biology and therapeutic implications DOI
Wenjie Chen, Yingjie Wang

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Language: Английский

Citations

0
Translating Preclinical Insights into Clinical Strategies: Targeting Cancer Stem Cells and Stemness in Prostate Cancer DOI
Najma Farahani,

Amin Maghsoodlou,

Mohammadarian Akbari

et al.

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155934 - 155934

Published: March 1, 2025

Language: Английский

Citations

0
CD44 is a macrophage receptor for TcdB from Clostridioides difficile that via its lysine-158 succinylation contributes to inflammation DOI Creative Commons
Zhuo Chen,

Wenzi Zhang,

Danni Wang

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: May 18, 2025

Toxin B (TcdB) is a critical virulence factor in Clostridioides difficile-associated disease (CDAD), which activates macrophages to promote inflammation and epithelial damage. However, the mechanism by TcdB targets inflammation-related receptors on macrophage surface underlying molecular mechanisms remain unknown. The frizzled-binding domain of (TcdB-FBD) promising target TcdB. Here, FBD was found trigger inflammation, similar TcdB, but did not induce cytotoxicity. Thus, using as bait protein, CD44 identified an receptor for TcdB/FBD. role confirmed CRISPR/Cas9-mediated gene knockout mice. Using 4-D label-free succinylation quantitative modification proteomics, we demonstrated that TcdB/FBD binds macrophages, promotes K158 via SUCLG2 suppression, enhances NF-κB translocation/transcriptional activity, thereby driving inflammation. Finally, blocking binding favorable strategy inhibiting TcdB-mediated This study only provides new therapeutic prevention treatment CDAD also elucidates inflammatory effect TcdB/FBD-CD44 axis.

Language: Английский

Citations

0
Molecular mechanisms driving lineage plasticity in prostate cancer: NANOG and beyond DOI Creative Commons

Hamed Maasoumyhaghighi,

Mansoureh Nouri,

Jinghui Liu

et al.

Published: Dec. 27, 2024

Developing resistance to androgen receptor (AR) signaling inhibitors is a significant challenge in the treatment of castration-resistant prostate cancer. Prolonged use like enzalutamide can cause cancer cells undergo lineage reprogramming, transitioning neuroendocrine subtypes that no longer rely on AR signaling. These are among most aggressive forms During this process plasticity, experience extensive transcriptional rewiring and acquire stem-like properties characterized by increased stemness. Research has shown gain these traits through expression stem cell-associated proteins such as NANOG, particularly under stable accumulating conditions. The post-translational modification NANOG at specific sites critical for maintaining its stability, which turn enhances tumorigenic potential cells. This review discusses mechanisms phosphorylation promotes stemness plasticity

Language: Английский

Citations

0
SAA1 released by tumor cells facilitates the aggressiveness of breast cancer by inducing reprogramming of molecular phenotypes in immune cells DOI Creative Commons

Jinkun Xia

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract Most breast cancer patients are diagnosed at an advanced stage and have a poor prognosis. Recurrence of tumor metastasis major obstacles to clinical treatment. It is imperative explore new diagnostic prognostic markers improve the early diagnosis outcomes cancer. Recently, metastatic cancers transcriptional signature reveals Serum amyloid A1 (SAA1), acute-phase apolipoprotein reactant, associated with in expression clinicopathological features. However, its regulatory function remains elusive, contribution uncertain. In this research, we downloaded mRNA-sequencing data from Gene Expression Omnibus (GEO) database (GSE102818, GSE28785, GSE134591) comprehensively investigate relationship between SAA1 impact on implications, further unveiled connection SAA1-mediated immunoregulation We found that implicated cell migration regulation immune cells by modulating cytokine-cytokine receptor interaction. Meanwhile, released was demonstrated contribute inducing adipocytes reprogramming. Several current viewpoints propose reprogramming molecular phenotype driver invasion microenvironment. Based previous studies our findings, hypothesized cellular may also apply microenvironment, interaction through release relevant aggressiveness cancer, which help patient decision-making for immunotherapy.

Language: Английский

Citations

0